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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... anti-PSEN1 (rat, MAB1563, Sigma-Aldrich, 1:500). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-tubulin YL1/2 (1:100, Sigma), mouse IgG2a anti-centrin (20H5 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-NCAM-L1 (1:500, Millipore# MAB5272), rabbit anti FoxP2 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... rat anti-HA-Peroxidase (3F10, Sigma, 1:2000), rabbit anti-HA antibody (H6908 ...
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bioRxiv - Neuroscience 2023Quote: ... rat antibody to Myc (4A6, 1:50, Millipore). To enhance the signal intensity of Beat-IIb-MiMIC-GFP and Beat-IV-MiMIC-GFP ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti-L1 (1:500, Millipore, catalog #MAB5272); rabbit anti-TBR1 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... and rat anti-laminin (1:400; Sigma L0663) primary antibodies diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-Ctip2 (1:500, Millipore-Sigma, MABE1045), rabbit anti-Satb2 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-Ctip2 (1:500, Millipore-Sigma, MABE1045), rabbit anti-Satb2 (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... laforin was expressed in BL21 (DE3) (Novagen) E ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Cell Biology 2020Quote: ... The GST-His6-tagged forms of LC3B-His12 and GATE-16-His12 proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed in Lysogeny Broth (LB ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Biophysics 2021Quote: ... The protein was expressed and purified the same way as SARS-CoV Mpro-2 but Factor Xa protease (Sigma, Canada) was used (4 °C ...
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bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Cell Biology 2019Quote: ... from bacteria were performed as previously described (Llauró et al., 2018) except that proteins were expressed in Rosetta 2(DE3)pLys cells (Novagen).
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were expressed by induction at exponential growth phase (OD600nm≈0.5) with 0.5 mM isopropyl-β-D-thiogalactopyranoside (Sigma, I6758) for 2.5 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Proteins guided towards high cysteine content were transformed into and expressed in Rosetta-gami B(DE3) Competent Cells (Novagen 71137). The 1 mL and 50 mL scale protein purification protocols were otherwise followed.
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bioRxiv - Cell Biology 2023Quote: Rho family GTPase proteins were expressed using a modified pGEX vector transformed into Rosetta-Gami B BL21(DE3) pLysS strains (Novagen). Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes ...
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bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... 15N] triple-labeled proteins for NMR assignments at pH 5.0 were expressed in M9 minimal medium prepared using 100% D2O (Sigma-Aldrich) as solvent (M9 D2O medium) ...
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bioRxiv - Molecular Biology 2021Quote: ... THP-1-derived macrophages were then stimulated for 24 hours with 1 μg/mL LPS (Sigma, St. Louis, MO), followed by a 24-hour incubation with 1×1010 particles/mL Exo-101 ...
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Systems Biology 2023Quote: ... THP-1 cells were stimulated with either 100ng/mL (for M1 macrophages) or 50ng/mL (for M2 macrophages) of phorbol 12-myristate 13-acetate (PMA, Sigma) for 48h ...
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bioRxiv - Neuroscience 2022Quote: ... commercially available ELISA kits were used to measure lactose content (Sigma-Aldrich Cat ...
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bioRxiv - Immunology 2021Quote: ... which was analyzed by enzyme linked immunosorbent assay (ELISA) kit (Sigma, MO).
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bioRxiv - Developmental Biology 2022Quote: ELISA kits were used for measurements Growth Hormone (Merck-SIGMA EZRMGH-45K) according to manufacturers’ instructions.
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bioRxiv - Neuroscience 2022Quote: ... S100B ELISA kits were purchased from Millipore (EMD Millipore, St. Louis, MO) and samples were diluted 1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... Plasma was processed using an insulin ELISA kit (Millipore Sigma; #EZRMI-13K) following manufacturer’s instructions in duplicate ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin B1 shRNA was expressed from pLKO.1 shRNA-LMNB1.71 puro (SHCLND-NM_005573, Sigma-Aldrich) containing the sequence ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: E-cad perturbations were expressed using the pLKO.1 non-target scramble (#SHC016, Millipore Sigma) and E-cad shRNA (TRCN0000237841 ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Physiology 2020Quote: ... and serum osteopontin (OPN) by Rat Single Plex Kit (Millipore). All the assays were performed in accordance with manufacturer protocols.
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the M2 macrophages were treated with 1 μg/mL lipopolysaccharides (LPS) (Sigma, Saint Louis, MO, USA) or 40 ng/mL IL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... GM-CSF cultured macrophages were polarized to M1 macrophages with 20 ng/ml IFN-y (Prepotech) and 100 ng/ml LPS (Sigma-Aldrich), while M-CSF cultured macrophages were polarized to M2d macrophages with 10 ng/ml M-CSF and 20 ng/ml IL-6 (Prepotech) ...
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bioRxiv - Immunology 2021Quote: ... were differentiated into macrophages for six days using 50ng/mL granulocyte-macrophage colony-stimulating factor and 25ng/mL interleukin-10 (Sigma, USA), as described previously [24].
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bioRxiv - Microbiology 2021Quote: ... for ELISA (Sigma) was added to visualize the reaction ...