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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Prior to macrophage challenge bacteria were opsonized in RPMI (Sigma-Aldrich) containing either 10% mouse immunized pooled serum or human immunized pooled serum with detectable levels of anti-pneumococcal antibodies for bone marrow-derived macrophages (BMDMs ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was extracted from mouse macrophages using TRI reagent (Sigma). Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C ...
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bioRxiv - Biochemistry 2023Quote: Macrophages were then removed from the plate using Cell Stripper (Sigma), washed ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... cells were detached using the macrophage detachment media (Sigma C-41330) and fixed with PrimeFLOW fixation buffer (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... RAW264.7 macrophage cells were treated with UK5099 (10 µM, Sigma-Aldrich, dissolved in DMSO 3 h prior to 1st or 2nd rounds of 2-AA exposure ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... THP-1 derived macrophages were obtained by differentiating THP-1 cells with phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) treatment at 100 nM for 24 h according to the previous study (Li et al. ...
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bioRxiv - Biochemistry 2019Quote: ... A synthetic gene encoding the KRAB domain (residues 1-71) from ZNF93 (UniProt: P35789) codon-optimized for E.coli was expressed from the pET20 plasmid (Novagen) with N-terminal Twin-StrepII and maltose binding protein (MBP ...
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bioRxiv - Cancer Biology 2021Quote: ... 1/500 goat anti-rat AlexaFluor555 and 1/500 goat anti-mouse AlexaFluor488 (both Millipore) were then incubated for 1.5 hour ...
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bioRxiv - Genetics 2021Quote: ... 1:250 rabbit anti-calcitonin gene-related peptide (CGRP) (Millipore PC205L-100UL), 1:250 rat anti-myelin basin protein (MBP ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified gene drmA was cloned in plasmid pACYCDuet-1 (Sigma-Aldrich: 71147), resulting in a non-tagged protein product ...
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bioRxiv - Microbiology 2022Quote: The C1-07020 gene was cloned into the plasmid pETDuet-1 (Novagen) and then transformed into E ...
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bioRxiv - Neuroscience 2019Quote: ... (6) mouse anti-calcitonin gene-related peptide (CGRP; 1:100, Sigma, USA) of neuronal peptide in the nervous tissues ...
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bioRxiv - Biochemistry 2022Quote: ... The csx30 gene was cloned into the pCDFDuet-1 (Novagen, streptomycin resistance) vector with an N-terminal hexahistidine tag and a C-terminal Strep II tag ...
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bioRxiv - Microbiology 2024Quote: Cas1 and cas2/3 genes were subcloned into pRSFDuet-1-MCS1 (Novagen) and pGEX6p-1 (Novagen) ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... THP-1 cells were differentiated to macrophages by supplementing the growth medium with 200 nM phorbol myristate acetate (PMA, Sigma) overnight.
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bioRxiv - Microbiology 2020Quote: ... Maturation of THP-1 cells into macrophages was induced by adding 200 nM PMA (phorbol 12-myristate 13-acetate; Sigma) in cell culture medium for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: THP-1 was differentiated into macrophages by the treatment with 100 ng/mL PMA (Sigma-Aldrich, St Louis, MO, USA) for 48 hours ...
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bioRxiv - Microbiology 2019Quote: ... supernatants were collected and analyzed for total GLP-1 levels by ELISA (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were extracted with the Gene Elute Plasmid Miniprep kit (Sigma, St. Louis, USA) and sent for sequencing to two different companies (EuroFins Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... COS-7 (hamster, DSMZ Germany, ACC 60), and Rat-1 (rat, ATCC, CRL-2210) cells were cultured in DMEM (Sigma-Aldrich D6429) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Microbiology 2021Quote: ... the antibodies isotypes were determined by ELISA using a mouse Ig isotyping kit (ISO-2, SIGMA).
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 125 μl of lysis buffer provided with a CAT ELISA kit (Sigma). CAT expression and Renilla luciferase activities were measured and the ratio was calculated.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Cancer Biology 2021Quote: ... rat IgG (Sigma), rabbit F(ab’)2 anti-mouse IgG conjugated to FITC (STAR9B ...
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bioRxiv - Neuroscience 2022Quote: ... rat astrocytes (Sigma) were plated in a DMEM media (Thermofisher ...
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Lineage, Identity, and Fate of Distinct Progenitor Populations in the Embryonic Olfactory EpitheliumbioRxiv - Developmental Biology 2021Quote: ... NCAM (Millipore, rat), and OMP (Pierce Biotech ...
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...
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bioRxiv - Plant Biology 2023Quote: ... Plant Total Protein Extraction Kit (Sigma-Aldrich) was used ...
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bioRxiv - Microbiology 2023Quote: ... the CelLytic MEM protein extraction kit (Sigma) was used as described elsewhere (López & Kolter ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Microbiology 2022Quote: ... The CF640-labelled ACE2 at 12–14 different concentrations (200 nM to 13 pM in PBS supplemented with bovine serum albumin at 1 g/l) per measurement were incubated with expressed yeast aliquots and 1 nM bilirubin (Sigma-Aldrich, Cat# 14370-1G) and analysed by using FACS S3e Cell Sorter device (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... and/or Goat anti-rat IgG (for anti-PSGL-1, Sigma Aldrich) and incubated for 10 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... and immunoblotted with primary rat anti-DAT (MAB369, Millipore; RRID:AB_2190413; 1:1000) overnight at 4°C followed by the addition of a goat anti-rat HRP-conjugated secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... rat monoclonal anti-Cytokeratin 19 (Sigma-Aldrich, Cat. No. MABT913, 1:500) and goat polyclonal anti-Albumin (Novus ...
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bioRxiv - Molecular Biology 2020Quote: ... a rat monoclonal antibody to the HA epitope (11867423001, Sigma, 1:100), or a rabbit polyclonal antibody to Sox9 (AB5535 ...
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bioRxiv - Cancer Biology 2020Quote: The following antibodies were used: laminin (rat, 1:1500, Sigma-Aldrich, L0663) and DAPI (1:3000).
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies used were: 1/3000 goat anti-rat HRP conjugate (Millipore), 1/10 000 goat anti-rabbit HRP conjugate (Millipore) ...
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bioRxiv - Immunology 2020Quote: ... 1×107 BM cells were blocked with purified rat IgG (Sigma-Aldrich) and stained for 30 minutes on ice with SM containing the following antibodies ...
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bioRxiv - Microbiology 2021Quote: ... we used rat anti-HA clone 3F10 (diluted 1:100, Sigma #2158167001) and mouse anti-dsRNA J2 (diluted 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-Rpb1-S2-P clone 3E10 (rat, monoclonal, Millipore, #04-1571-1), anti-Rpb1-S5-P clone 3E8 (rat ...