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Citations for Millipore Sigma :
401 - 450 of 5985 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... After purification using spin-desalting columns (40K MWCO) in PBS (Sigma-Aldrich, D8537-500ML), modified goat-anti-rabbit IgG (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... a set of CasMam oligonucleotides was designed and synthesized (Sigma-Aldrich/Merck, desalt purification). The oligonucleotides contained the optimized linker sequences (α/β/γ/δ/ε/ω ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Microbiology 2023Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, USA) for one to two hours and washed four times ...
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bioRxiv - Molecular Biology 2022Quote: Eluates from affinity purification were mixed with four times ice-cold Acetone (Sigma Aldrich) and NaCl for precipitation of proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... Water was purified with a Milli-Q Simplicity 185 Water Purification System (Merck Millipore). All other chemicals and solvents obtained from commercial suppliers were used without further purification ...
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bioRxiv - Molecular Biology 2023Quote: ... a portion of a purification was extensively concentrated (Microcon Ultracel YM-10, Amicon-Millipore), quantified by SDS– PAGE/Coomassie staining and then used as standard for Western blot quantification of the purified complex in that and further purifications.
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bioRxiv - Cancer Biology 2023Quote: ... Water was purified with a Milli-Q Simplicity 185 Water Purification System (Merck Millipore). All other chemicals and solvents obtained from commercial suppliers were used without further purification ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Biophysics 2023Quote: ... Purification of folded structures was performed with Amicon centrifugal filters (Merck Millipore, cat.no.: UFC5100) and TAE buffer with 5 mM MgCl2 in five washing steps at 8 000 rcf for 4 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by purification using phenol chloroform (pH 8.0) and Ultrafree-MC Centrifugal Filters (Millipore).
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bioRxiv - Microbiology 2024Quote: ... 100mM NaCl by batch purification with EZviewTM Red ANTI-FLAG M2 Affinity gel (Sigma) and eluted with 3XFLAG peptide (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Libraries were then treated with sodium bisulphite according to the manufacturer’s instructions (Imprint DNA Modification Kit; Sigma, MOD50) and amplified by PCR (10 cycles ...
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bioRxiv - Physiology 2021Quote: ... DNA fragments were directly purified from the PCR mix using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Gibson assemblies were performed with the NEBuilder HiFI DNA Assembly Master mix (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... The pJFRC19 backbone and TkR86C fragments were ligated using Roche Rapid DNA Ligation Kit (Millipore Sigma cat#11635379001). The recovered TkR86C coding sequences (isoform B ...
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bioRxiv - Microbiology 2022Quote: ... salivarius DPC6487 culture cell pellets using a GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich; Co. Wicklow, Ireland). The purity and concentration of genomic DNA was confirmed using the NanoDrop 1000 (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse genotypes were determined by PCR using genomic DNA extracted using the REDExtract-N-Amp kit (Sigma XNAT) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... mice DNA was extracted from ear notches or tails using the RED ExtractN-Amp Tissue PCR Kit (SIGMA). For Lrp1flox ...
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bioRxiv - Immunology 2024Quote: ... Calf thymus DNA (CT-DNA) was purchased from Millipore, Merck ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% SDS) and the supernatant was subjected to affinity purification with anti-HA antibody (Sigma) conjugated to magnetics beads ...
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bioRxiv - Biophysics 2022Quote: ... Deionized water was obtained from a Milli-Q water purification system (Millipore, Bedford, MA, U.S.A.). The resistivity of deionized water used was 18.2 MΩ cm.
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bioRxiv - Microbiology 2019Quote: ... The purification of virions was confirmed by SDS-PAGE and Coomassie brilliant blue (Sigma-Aldrich) staining.
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bioRxiv - Microbiology 2020Quote: ... 0.1% SDS) and the supernatant was subjected to affinity purification with anti-HA antibody (Sigma) or anti-GFP antibody (Invitrogen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... For the nextera-based libraries a secondary purification was performed using magnetic SpeedBeads™ (Sigma) dissolved in 0.44mM PEG8000 ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). Peptides recovered from in-gel digestion processing were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Cancer Biology 2022Quote: ... Selected fractions were combined and concentrated with an Amicon Pro Purification system (10 kDa, Millipore). All samples were stored at −80 °C ...
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bioRxiv - Immunology 2022Quote: ... purification of monocytes was performed using a hyper-osmotic density gradient medium Percoll (Sigma-Aldrich). The PBMC suspension was adjusted to a concentration of 50-60x106 cells/mL and subsequently applied on top of the hyper osmotic Percoll solution ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). The peptides recovered were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 µL of ultrapure water (supplied by a MilliQ Advantage A10 purification system, Merck Millipore) was added prior to injection and the total dilution was x10 ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were subjected to purification using either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Water was purified by a Milli-Q™ ultraviolet purification system (Millipore, Bedford, MA, USA). Dimethyl sulfoxide was obtained from Sigma–Aldrich (Saint Louis ...
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bioRxiv - Neuroscience 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). The peptides recovered were reconstituted into a final concentration of 0.5µg/µL of 2% acetonitrile ...
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bioRxiv - Neuroscience 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). Peptides recovered from in-gel digestion processing were reconstituted into a final concentration of 0.5µg/µL of 2% acetonitrile ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by RNase and ProteinaseK digestion and purification using Phenol-Chloroform Isoamyl Alcohol mix (Millipore) and PhaseLock tubes (5PRIME) ...
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bioRxiv - Immunology 2023Quote: ... It was prepared in-house using a Milli-Q Direct Water Purification System (Millipore-Sigma). Phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... It was prepared in-house using a Milli-Q Direct Water Purification System (Millipore-Sigma). Phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Ultrapure water supplied by a Milli-Q® Reference Water Purification System (Merck Millipore Corp.) was used for buffer preparation ...
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bioRxiv - Cell Biology 2021Quote: Apoptotic cells were visualized with the terminal deoxynucleotidyl transferase (TdT)-FragEL DNA Fragmentation Detection Kit (Millipore, Etobicoke, ON, Canada) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed with 0.5% SDS in EB buffer and bisulphite treated with the Imprint DNA Modification kit (Sigma). The resulting DNA was purified using columns and reagents from the EZ DNA Methylation Direct kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: Yolk sacs were dissected from embryos and used for DNA extraction with the Red Extract-N-Amp kit (Sigma). Usp9x status was assessed by PCR using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... gDNA for illumina sequencing of the eleven other isolates was extracted using a Genelute Plant DNA Miniprep Kit (Sigma) using the manufacturers protocol with the following modifications ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated in an endotoxin-free manner (GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit; Sigma-Aldrich) and concentrated using paramagnetic beads (HighPrep PCR Clean Up System ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma). Hybridization with a 5’ probe produced an 8.1 Kb band from the WT and a 5.1 Kb band from the targeted locus ...
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bioRxiv - Genetics 2019Quote: ... the tagmented DNA was amplified by PCR using the KAPA2G Robust PCR kit (Sigma-Aldrich, St. Louis, MO, USA) using the GC buffer along with 0.2 μM of each custom P5 and P7 indexing primers (File S1 ...
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bioRxiv - Microbiology 2019Quote: ... each RNA sample was supplemented with 1 unit of DNase I (from the DNA amplification grade Kit of Sigma) per µg of RNA and incubated at room temperature for 10 min ...
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bioRxiv - Neuroscience 2019Quote: DNA from offspring was extracted from mouse ear punches using Extract-N-Amp® kit (Sigma, St. Louis, MO) and genotyped using a multiplex PCR reaction ...
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bioRxiv - Developmental Biology 2020Quote: Genomic DNA extractions were performed with Extract-N-Amp or REDExtract-N-Amp™ Tissue PCR Kits (Sigma-Aldrich). Whole embryos or adult zebrafish fin-clips were lysed by incubation in a mixture of 50 μL extraction solution (Extraction Solution E7526 ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmid DNA was purified with a NucleoBond Xtra Midi kit (Cultech, 22740410.50) and resuspended in nuclease-free water (SIGMA). Other plasmids used were pCMV-GFP expressing GFP under the CMV promoter ...
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bioRxiv - Genetics 2021Quote: ... These kits were used to purify DNA from crude lysates or from tissue samples preserved in RNAlater solution (Sigma).