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Citations for Millipore Sigma :
651 - 700 of 5985 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... ten first or second leaves from independent plants were harvested at the indicated time points and transferred to a 15 ml tube containing 6 ml water purified using a Milli-Q integral 5 water purification system (Millipore). After a 6 h-incubation at room temperature on a horizontal shaker ...
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bioRxiv - Microbiology 2019Quote: ... and water purified to a resistivity of 18.2 MΩ·cm (at 25 °C) using a Milli-Q Gradient ultrapure water purification system (Millipore, Billerica, MA) were used to prepare mobile phase solvents ...
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bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
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bioRxiv - Biochemistry 2021Quote: ... Samples from the key steps of the purification were subjected to western blot analysis (anti-3x-FLAG antibody A8592, Sigma) (Supplementary Fig ...
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bioRxiv - Immunology 2021Quote: ... Purified immunoglobulin (Ig) was purified using an antibody purification system based on protein A-affinity chromatography (PURE-1A; Sigma-Aldrich). The manufacturer’s instructions were as described in [8] ...
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Cell Biology 2022Quote: ... copious rinsing using ultra-pure water (resistivity >18 MΩ·cm) from a Milli-Q Advantage A-10 purification system (EMD Millipore) was performed after each step ...
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bioRxiv - Biophysics 2022Quote: ... Water was purified to a resistivity of 18.2 MΩ·cm (at 25 °C) using a Milli-Q Gradient ultrapure water purification system (Millipore, Billerica, MA). DNA sequencing was performed at the UC Berkeley DNA Sequencing Facility ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved His-tagged GB1 and remaining TEV were removed via a second Ni-NTA purification after buffer exchange to Buffer A using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S200 Increase 26/600 column equilibrated in HEPES buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Zfp106 and Zfp106[WD40+ZnF] proteins were purified as described above and then buffer exchanged to 10 mM sodium cacodylate (pH 7.35) and concentrated using the Amicon Pro Purification System (Millipore, Sigma). Three scans were recorded and averaged for each sample ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was used for FLAG purification in pull-down assays with Anti-Flag® M2 Magnetic Beads (Sigma-Aldrich), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen ...
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bioRxiv - Biochemistry 2023Quote: ... uncleaved protein and TEV were subsequently removed by an ortho nickel affinity purification step and the sample concentrated to < 2 mL using a 10K Amicon Ultra-15 concentrator (Millipore). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... and water purified to a resistivity of 18.2 MΩ·cm (at 25 °C) using a Milli-Q Gradient ultrapure water purification system (Millipore, Billerica, MA) were used to prepare LC mobile phase solvents ...
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bioRxiv - Bioengineering 2023Quote: ... Ultrapure water having a resistivity of >18.2 MΩ.cm was produced from a Milli-Q water purification system (Merck Millipore, Bayswater, Australia). Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Cleaved His-tagged GB1 fragment and remaining TEV were removed via a second Ni-NTA purification after buffer exchange to buffer A using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex 200 Increase 26/600 column equilibrated in HEPES buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... and the supernatant containing the solubilized protein was collected for affinity purification by batch-binding to 10 mL of M2-agarose FLAG resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... the reactions were desalted by ion pairing reverse phase (IP-RP) purification using C18 ZipTip pipette tips (Millipore, Billerica, MA) to avoid smearing of bands in PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA sonication and immunoprecipitation of the protein/DNA complexes with specific antibodies (anti-Flag M2, Sigma) or without antibodies as a negative control ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001). After 24 to 40 hours of transfection cells were mounted and imaged on an Olympus FV1000 confocal microscope (40x Uplan FL N oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001). After 24 to 40 hours of transfection cells were mounted and imaged on a Nikon A1 confocal microscope (Plan Fluo 40x oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... 0.324 and 0.108ng of a reference DNA (Human genomic DNA from buffy coat, Sigma cat# 11691112001) are included in each PCR run so that the quantity of targeted templates in each research sample can be determined relative to the reference DNA sample by the standard curve method ...
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bioRxiv - Plant Biology 2019Quote: ... We extracted DNA from our yeast mannitol media cultures using GenElute Bacterial Genomic DNA (Millipore-Sigma). We sent 10 ng of DNA of each field and culture sample to Genome Québec (McGill University ...
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bioRxiv - Plant Biology 2019Quote: ... We extracted DNA from our yeast mannitol media cultures using GenElute Bacterial Genomic DNA (Millipore-Sigma). We sent 10 ng of DNA of each field and culture sample to Genome Québec (McGill University ...
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bioRxiv - Plant Biology 2019Quote: ... 20 μl salmon sperm DNA (Sigma #D1626 ...
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bioRxiv - Biophysics 2020Quote: ... and the DNA with DAPI (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... RNA oligonucleotides were purchased from Eurogentec and DNA oligonucleotides from Sigma-Aldrich. All oligonucleotides were HPLC purified ...
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bioRxiv - Bioengineering 2022Quote: ... single strand DNA (Sigma-Aldrich, D8899) were coated onto 96-well half-area high- binding ELISA plates (Corning ...
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bioRxiv - Biophysics 2022Quote: ... Calf thymus DNA (ctDNA) (Sigma-Aldrich) was dissolved following the manufacture’s instructions to obtain a 1 mg/ml stock solution ...
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bioRxiv - Cell Biology 2019Quote: ... with calf thymus DNA (D4764, Sigma) as reference.
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bioRxiv - Plant Biology 2019Quote: ... JumpStart Taq DNA polymerase (Sigma-Aldrich) and SYBR-GreenI (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... and JumpStart Taq DNA Polymerase (Sigma) once per month to ensure cells were mycoplasma negative ...
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bioRxiv - Bioengineering 2021Quote: ... single strand DNA (Sigma-Aldrich, D8899) were coated onto 96-well half-area high-binding ELISA plates (Corning ...
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bioRxiv - Biochemistry 2022Quote: ... DNA primers were purchased from Sigma.
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bioRxiv - Cancer Biology 2019Quote: ... and JumpStart Taq DNA Polymerase (Sigma) once per month to ensure cells were mycoplasma negative.
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bioRxiv - Synthetic Biology 2020Quote: Calf thymus DNA (Sigma D1501-100MG) was diluted in 10 mM Tris-Acetate pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... DNA was counterstained with DAPI (Sigma) and actin labelled with TRITC-conjugated phalloidin ...
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bioRxiv - Immunology 2019Quote: HPLC-grade DNA oligos (Sigma-Aldrich) were dissolved in sterile endotoxin-free water ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was labeled with Hoechst33342 (Sigma). Images were collected using a 63X ...
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bioRxiv - Microbiology 2020Quote: ... and herring sperm DNA (Sigma Aldrich). Probes were resuspended in 10 μL formamide ...
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bioRxiv - Immunology 2020Quote: ... double stranded DNA (Sigma-Aldrich; D4522), insulin (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... ordered as complementary DNA oligonucleotides (Sigma) with overhangs for BbsI ...
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bioRxiv - Immunology 2022Quote: ... HT-DNA (Sigma-Aldrich, Cat#D6898), Lipofectamine 2000 (Life Technologies ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... and Jumpstart Taq DNA polymerase (Sigma). The following PCR parameters were used ...
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bioRxiv - Immunology 2022Quote: ... DNA using DAPI (Sigma-Aldrich, USA) and F-actin using Alexa Fluor 680 phalloidin (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and DNA with DAPI (D9542, Sigma). Appropriate secondary antibody was FITC-conjugated donkey-anti-rabbit immunoglobulins (Jackson Laboratories) ...
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bioRxiv - Plant Biology 2023Quote: ... using KOD DNA polymerase (Merck Millipore). All plasmids were verified by sequencing.
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bioRxiv - Immunology 2023Quote: ... double stranded DNA (dsDNA) (Sigma, D4522), single stranded DNA (ssDNA ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-DNA (Sigma; 1:800) antibodies in 40 µL PBS for 30 min at room temperature ...