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Citations for Millipore Sigma :
451 - 500 of 5985 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Plasmid library DNA was purified from twice 5 ml of culture using a GenElute Plasmid Miniprep Kit (Sigma-Aldrich) with elution in twice 20 µl of ddH2O ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from mouse tail tip samples using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR was performed using the REDExtract-N-Amp PCR Readymix (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... Whole genome amplification was performed using SeqPlextm Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from a small piece of Arabidopsis leaf using Extract-N-Amp extraction kit (Sigma-Aldrich) and used as a template for PCR ...
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bioRxiv - Biochemistry 2020Quote: DNA primers (Sigma) were radiolabeled at the 5’-end with 32P using the manufacturer recommended protocol for T4 PNK (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: DNA (Sigma-Aldrich) and RNA (Dharmacon ...
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bioRxiv - Microbiology 2020Quote: ... Integrated viral DNA was quantified by first pre-amplifying the DNA using AccuTaq™ LA DNA Polymerase (Sigma) using the following primers ...
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bioRxiv - Biophysics 2020Quote: ... All water used was purified with a Milli-Q Ultrapure water purification system (Millipore, Bedford, MA).
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bioRxiv - Molecular Biology 2019Quote: ... Mitochondria were lysed and immunoaffinity purification was carried out with FLAG M2 affinity gel (Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Cold APC purification buffer (100 mM NaPi, Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333 ...
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bioRxiv - Plant Biology 2021Quote: ... Affinity purification of tagged RPL18-containing polysomes was carried out using anti-FLAG beads (Sigma-Aldrich) incubated with the supernatant at 4 °C for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and desalted using an Amicon 10 kDa MWCO centrifugal filter prior to purification (Millipore-Sigma, USA).
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bioRxiv - Biochemistry 2022Quote: ... and desalted using an Amicon 10 kDa MWCO centrifugal filter prior to purification (Millipore-Sigma, USA).
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bioRxiv - Cell Biology 2022Quote: ... α-Lactose-Agarose beads used for galectin-8 purification were purchased from Sigma (Catalog no. L7634). The glass-bottomed petri-dishes (MatTek ...
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bioRxiv - Synthetic Biology 2022Quote: AHLs were purchased from Sigma and used without further purification as follows: 3-oxohexanoyl-L-homoserine lactone (3OC6-HSL, K3007, Sigma) for the lux system ...
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bioRxiv - Biochemistry 2020Quote: Deionised water was purified in-house by DIRECT-Q 3UV water purification system (Millipore, Watford, UK). Methanol ...
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bioRxiv - Microbiology 2021Quote: ... RNA purification from pelleted worms is performed using phenol-chloroform extraction (TRI reagent®, Sigma-Aldrich) with the same protocol described above ...
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bioRxiv - Genetics 2020Quote: ... The Rad21l1 peptide was purified under denaturing conditions using Novagen NiNTA purification resins (Sigma, Catalog #: 70666) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... α-Lactose-Agarose beads used for galectin-8 purification were purchased from Sigma (Catalog no. L7634). Galectin-8 mutated forms were generated as previously described (Levy et al. ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.4 for affinity purification of the FLAG-tagged cHMM on M2 mAb-Sepharose beads (Sigma). Bound cHMM was eluted with 0.1 mg⋅mL−1 FLAG peptide (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ultra-pure water was produced by a Millipore A10 Milli-Q water purification system (Millipore, USA).
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bioRxiv - Biophysics 2022Quote: ... Ultrapure H2O was obtained from a Milli-Q IC 7000 purification system (Millipore Sigma, Burlington, MA).
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Pathology 2023Quote: ... Deionized water was provided in-house by a water purification system from EMD Millipore (Billerica, MA). Phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
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bioRxiv - Biochemistry 2023Quote: Deionised water was purified in-house by DIRECT-Q 3UV water purification system (Millipore, Watford, UK). Methanol ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Molecular Biology 2024Quote: ... purification was performed using dynabeads conjugated in-house with anti-FLAG M2 monoclonal antibody (Sigma-Aldrich). Beads conjugation was performed with a procedure adapted from previous protocols (61) ...
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bioRxiv - Immunology 2022Quote: ... Calf thymus DNA (CT DNA) was purchased from Sigma (#D1501-100MG).
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bioRxiv - Neuroscience 2022Quote: ... Taq DNA polymerase and phusion DNA polymerasewere purchased from Sigma (USA). DNA ladders ...
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bioRxiv - Microbiology 2020Quote: ... This was then followed by genomic DNA removal and cleaning using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Genomics 2020Quote: Between 250 ng and 2 µg of gDNA extracted from each tissue sample was bisulfite treated with an Imprint DNA Modification Kit (Sigma). Control samples lacking DNA template were run to ensure that there was no contamination during the bisulfite conversion of DNA ...
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bioRxiv - Genetics 2020Quote: Bisulphite conversions were carried out using the two-step modification procedure of the Imprint® DNA Modification Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted from 5 individual embryos at mid-gastrula (st. 11) or later employing the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma). The genomic region containing the CRISPR / Cas9 targeted region was PCR amplified using a nested PCR approach (Table 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and DNA was extracted as per the protocol in the Actetyl-Histone H3 Immunoprecipitation (ChIP) assay kit (Millipore, Billerica, MA). Anti-acetyl-Histone H3 antibody was used for the immunoprecipitation (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted as per manufacturer instruction and genomic DNA was removed using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Plant Biology 2021Quote: The coding sequences of most of the 44 non-pseudogene AtUMAMIT were amplified by PCR (oligonucleotide sequence is provided in Supplemental Table 1) by using the KOD Hot Start DNA Polymerase kit (Novagen) from a mix of reverse-transcribed RNA extracted from seedlings and inflorescences ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole genome amplification was performed using SeqPlex™ Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Microbiology 2020Quote: ... validated as hex1 deletion mutants by spore PCR and Southern blot analysis (DIG DNA Labeling and Detection Kit, Sigma-Aldrich), and stored as 25% glycerol stocks at −80 °C.
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bioRxiv - Neuroscience 2020Quote: ... All DNA fragments were cleaned-up with GenElute™ PCR Clean-Up Kit (Sigma Aldrich Co., St. Louis, MO, USA) prior to PCR analysis ...
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bioRxiv - Pathology 2020Quote: ... and methyl cytosine levels assessed by a specific primary and secondary HRP-conjugated antibody system following the manufacturer’s instructions (Imprint® Methylated DNA Quantification Kit-Sigma). Following development with TMB ...
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bioRxiv - Neuroscience 2021Quote: ... was isolated from 2 × 106 MØP cells in accordance with the instructions of the GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma). Separate PCR reactions were prepared using the GoTaq® Master Mix (Promega ...
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bioRxiv - Genetics 2020Quote: ... CRISPR-targeted regions were amplified with MiSeq-compatible gene-specific primers containing Read1 and Read2 adaptor sequences (IDT) using KOD DNA polymerase Hot start kit (TOYOBO/Millipore). The second PCR was performed with index adaptor primers compatible with Ilumina MiSeq (i5 and i7 primers ...
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bioRxiv - Genomics 2019Quote: Whole genome amplification was performed using SeqPlex™ Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cell Biology 2021Quote: ... A DNA probe against the NST1 region within the rDNA was synthesized using the Fluorescein-High Prime kit (Sigma-Aldrich). The fluorescein-labelled probe hybridization was carried out overnight at 68 °C ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Genomics 2022Quote: ... Genome extraction was then performed using the GenElute Bacterial Genomic DNA (gDNA) extraction kit (Sigma-Aldrich, St. Louis, MO, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...