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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2022Quote: The protein content was analysed with the Bicinchonic Acid Protein Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol with minor adjustments ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total carbonylated proteins were quantified using OxyBlot Protein Oxidation Detection Kit (Millipore, Billerica, MA) following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein precipitation was carried out using ProteoExtract Protein Precipitation Kit (EMD Millipore, 539180-1KIT) according to the manufacturer’s protocol and submitted for MS analysis to the Taplin Biological Mass Spectrometry Facility.
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were visualized using ECL kit (Millipore).
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bioRxiv - Cell Biology 2024Quote: The Protein-G CoIP kit (IP50, Sigma Aldrich) was used for coimmunoprecipitation (CoIP ...
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bioRxiv - Neuroscience 2020Quote: ... or mHTT protein (1-82aa, Millipore, MAB5492, 1:500), and the appropriate biotinylated goat anti rabbit or goat anti mouse or secondary antibody (Vector Laboratories ...
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bioRxiv - Immunology 2022Quote: Stable knockdown of host proteins in THP1 cells was carried out by transduction with lentivectors expressing individual shRNAs (pLKO.1, 400 ng p24Gag (as measured by ELISA) per 1 × 106 cells) in the presence of polybrene (Millipore). Cells were washed and cultured for 5-7 days in the presence of puromycin (2 µg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... For the inhibition of Thymidylate kinase (DTYMK) cells were incubated for 72 h after adding 2 μM (final conc.) of the YMU-1 compound (Sigma-Aldrich), solved in 15 % DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Immunology 2023Quote: Cells obtained by crawl-out were incubated for 24h at 37°C with the phosphatidylinositol-3-kinase inhibitor wortmannin or the carnitine palmitoyltransferase-1 inhibitor etomoxir (both from Sigma-Aldrich), at 10 and 200μM respectively.
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bioRxiv - Microbiology 2021Quote: ... the free RNA not associated with virions was removed from the samples using the Benzonase nuclease (Sigma), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and the second parotid salivary gland was analyzed using the pyruvate kinase activity assay kit (cat. No. MAK072, Sigma-Aldrich, St. Louis, MO). For both activity assays ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Cell Biology 2023Quote: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the levels of mouse HGF (Sigma-Aldrich, RAB0214), mouse EGF (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... The kinase domains were expressed in Escherichia coli ROSETTA (DE3) cells (Novagen) with TEV cleavable N-terminal GST and a non-cleavable C-terminal His6-tag ...
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bioRxiv - Cell Biology 2020Quote: ... kinase assays were prepared using C18 ZipTips (EMD Millipore, Etobicoke, ON, Canada). Samples in 50% acetonitrile and 0.1% formic acid were introduced into a linear ion-trap mass spectrometer (LTQ XL ...
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bioRxiv - Biophysics 2020Quote: ... 0.04 U/μl pyruvate kinase/ 0.05 U/μl lactic dehydrogenase (Sigma-Aldrich) and 5 mM ATP ...
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bioRxiv - Cell Biology 2021Quote: ... kinase assays were prepared using C18 ZipTips (EMD Millipore, Etobicoke, ON, Canada). Samples in 50% acetonitrile and 0.1% formic acid were introduced into a linear ion-trap mass spectrometer (LTQ XL ...
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bioRxiv - Biochemistry 2019Quote: ... 100 units/mL lactate dehydrogenase and 80 units/mL pyruvate kinase (Sigma) in buffer composed of 50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.05 mg/mL pyruvate kinase from rabbit muscle (Sigma-Aldrich, Schnelldorf, Germany), 0.5 mM NADH) ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 U/mL nucleoside 5-diphosphate kinase (Sigma, cat. Num. N2635) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μM glycogen synthase kinase (GSK) inhibitor (Calbiochem, D00163483 or Sigma, CHIR99021), 10 μM MEK inhibitor (Tocris ...
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bioRxiv - Biochemistry 2024Quote: ... 0.04 U/μl pyruvate kinase/0.05 U/μl lactic dehydrogenase (Sigma-Aldrich) and 5 mM ATP ...
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bioRxiv - Cancer Biology 2024Quote: CAMK2A D135N kinase domain was recombinantly expressed in BL21(DE3) cells (Millipore) as previously described32 ...
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bioRxiv - Biochemistry 2020Quote: ... For ELISAs with asialofetuin (Sigma Aldrich # A4781), 5 μg/mL was immobilized ...
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bioRxiv - Physiology 2023Quote: ... Insulin was measured by ELISA (Millipore Sigma). For glucose tolerance tests ...
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bioRxiv - Immunology 2022Quote: ... IL-10 and TNF-α were determined in 24 h culture supernatant of UNT control and treated MOs using respective ELISA kits (Sigma-Aldrich kit, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Cell Biology 2023Quote: ... immunoreactive proteins were detected using Immobilon Western Kit (Millipore). Protein bands from each blot were observed by an imaging system (ChemiDoc XRS+ ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using enhanced chemiluminescent detection kit (Millipore). To detect changes in the protein glycosylation ...
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bioRxiv - Molecular Biology 2023Quote: ... Elutions were precipitated using ProteoExtract Protein precipitation kit (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Protein was estimated using BCA kit (Sigma Aldrich, USA) and 20 µg protein was loaded on 12% SDS-PAGE gel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the BCA kit (Sigma), except for insoluble fractions due to due to formic acid’s denaturation effect ...
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bioRxiv - Microbiology 2022Quote: ... coli 83972 (bei Resources) associated with asymptomatic bacteriuria (ABU) were grown in static Luria-Bertani (LB, Sigma Aldrich) broth at 37°C for 48h to induce expression of type 1 pili ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% protein phosphatase inhibitor cocktail (Sigma). Lysed tissues were incubated at 4°C for 20 min and centrifuged at 15,000 rpm for 15 min ...