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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... along with an ATP regeneration system consisting of 187.5 U mL−1 pyruvate kinase and 50 mM phosphoenolpyruvate (Sigma). For gel degradation assays ...
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bioRxiv - Immunology 2022Quote: ... 20 µM DTT and phosSTOP in the presence of recombinant cyclin A2/cyclin-dependent kinase (CDK)1 (Sigma-Aldrich) and 10 mM ATP incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Kinase-treated cells were then labeled with a 1:1000 dilution of biotinylated 4G10 Platinum anti-phosphotyrosine antibody (Millipore) for an hour on ice and washed with PBS containing 0.1% BSA and 2 mM EDTA (isolation buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... v-abl transformed Bcl2/pMX-INV infected pro-B cells (1 million per ml) were treated with 0.5 uM of the v-abl kinase inhibitor ABLki (PD180970, Sigma) and assayed for rearrangement by FACS analysis of GFP expression at 0 ...
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...
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bioRxiv - Microbiology 2023Quote: ... the CelLytic MEM protein extraction kit (Sigma) was used as described elsewhere (López & Kolter ...
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bioRxiv - Plant Biology 2023Quote: ... Plant Total Protein Extraction Kit (Sigma-Aldrich) was used ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Immunology 2020Quote: ELISA plates (Cat. M5785, Sigma Aldrich) were first coated overnight at 4°C with 10μg/ml of each B cell peptide epitope ...
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bioRxiv - Molecular Biology 2023Quote: ... Ghrelin ELISA (EZRGRA-90K)(Sigma Aldrich) IGF-1 ELISA (Catalog #80574 ...
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bioRxiv - Immunology 2022Quote: – Antibody enzyme conjugates for ELISA (Sigma): Peroxidase conjugated Anti-mouse polyvalent immunoglobulins (IgG ...
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bioRxiv - Physiology 2020Quote: ... pancreatic glucagon and GLP-1 content were measured using glucagon ELISA (EZGLU-30K, Merck Millipore), or RIA kit (250-tubes GL-32K ...
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bioRxiv - Bioengineering 2020Quote: ... To make stock solution of S1P associated with fatty acid-free Bovine Serum Albumin (BSA) (Sigma) carrier proteins ...
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bioRxiv - Bioengineering 2019Quote: ... and complement C5a levels in serum were quantified by ELISA (Histamine kit: Enzo Life Sciences, Farmingdale, NY; Complement C3a kit: Quidel, San Diego, CA; Complement C5a kit: Sigma-Aldrich, St. Louis, MO). Spleen tissues were homogenized with a FastPrep-24 instrument in RIPA buffer supplemented with EGTA ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested and confirmed to be mycoplasma free using the Mycoplasma PCR ELISA kit (Sigma). CENP-A overexpression was induced by the addition of Dox to typical growth media at 10ng/ml (considered 1X) ...
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bioRxiv - Neuroscience 2021Quote: ... The BDNF of offspring hippocampus was measured using the ChemiKineTM BDNF Sandwich ELISA kit (CYT306; Chemikine, Millipore). A total of 80 µg/well of hippocampus protein was used and samples were analysed in duplicates ...
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bioRxiv - Cell Biology 2019Quote: ... The CRP concentration in mouse serum was measured using the mouse CRP ELISA kit (Sigma-Aldrich, RAB1121) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: Cell proliferation activity was measured by BrdU incorporation assay using a Cell Proliferation ELISA kit (Sigma-Aldrich). The wells of 96-well plates were seeded with 5000 HSC-2 or HO-1-N-1 cells ...
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bioRxiv - Genetics 2021Quote: ... leptin and adiponectin were measured in plasma from fasted mice using ELISA kits from Millipore (EZRMI-13K), Mercodia (10-1281-01) ...
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bioRxiv - Cancer Biology 2023Quote: ... Albumin was measured in the cell culture medium by Human ALB/Serum albumin ELISA Kit (Sigma-Aldrich) and in the cell lysate by qPCR ...
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bioRxiv - Neuroscience 2024Quote: Neuropeptide Y levels in tissue were measured with commercially available ELISA kit (EMD Millipore, EZHNPY-25 K). Mice were anesthetized with isoflurane and euthanized by decapitation ...
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bioRxiv - Microbiology 2020Quote: ... and 4 U of pyruvate kinase (from rabbit muscle, Sigma-Aldrich). If not stated otherwise ...
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bioRxiv - Evolutionary Biology 2019Quote: ... saturating amounts of pyruvate kinase and lactate dehydrogenase (Sigma-Aldrich, P0294), and varying amounts of ATP (0.01 to 8 mM) ...
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bioRxiv - Genomics 2019Quote: The HIPK kinase family inhibitor A64 trifluoroacetate was purchased from Sigma, and the MAP4K2 inhibitor PF06260933 ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 μg/mL of creatine kinase (Sigma-Aldrich, C3755-3.5KU)) ...
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bioRxiv - Cancer Biology 2023Quote: ULK1 and ULK2 Kinase inhibitor MRT68921 (0.5 μM; Sigma #SML1644-5MG), STING agonist 2’3’-cGAMP (2.5µg/mL ...
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bioRxiv - Cell Biology 2023Quote: Kinase inhibitors used are CDK4/6 inhibitor (PD 0332991, Palbociclib, Sigma); MEK/ERK inhibitor U0126 (Calbiochem) ...
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bioRxiv - Cell Biology 2024Quote: ... Kinase inhibitors used are CDK4/6 inhibitor (PD 0332991, Palbociclib, Sigma); MEK/ERK inhibitor U0126 (Calbiochem) ...
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bioRxiv - Cell Biology 2024Quote: ... Kinase inhibitors used: CDK4/6 inhibitor (PD 0332991, Palbociclib) from Sigma; MEK/ERK inhibitor U0126 from Calbiochem (San Diego ...
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bioRxiv - Plant Biology 2020Quote: ... AtMPK4 and AtMPK6 was performed using anti-MAP kinase-antibody (1:20000 dilutions) as primary antibody and anti-mouse IgG (Sigma) protein (1:15000 dilutions ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 0.5 µg/well of pLKO.1-puro Vector empty or with plasmids containing the target shRNA sequence for the indicated kinases (Mission Library, Sigma). 48h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Microbiology 2022Quote: ... The fraction of proteins >1 kDa was obtained by dialysis using the Mini Dialysis Kit 1 kDa (Sigma #GE80-6483-94). All kits were applied according to the manufacturer’s instructions.
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... Insulin serum was measured using ELISA (Rat/Mouse Insulin ELISA, #EZRMI-13K, Millipore St. Charles, MO, USA)) ...
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bioRxiv - Cell Biology 2023Quote: ... and Apo-1 protein sequence (Sigma) along with Proteome Discoverer™ contaminant database ...