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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... The DNP signal associated with RyR was determined using a specific anti-DNP antibody according to the manufacturer’s instructions (Millipore, Billerica, MA). All immunoblots were developed and quantified using an Odyssey system (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were visualised by SDS-PAGE using 12% TruPAGE precast gels and the associated 4X LDS sample buffer and 1X TEA-Tricine running buffer (Sigma-Aldrich). 5 µl Spectra™ Multi-Color Broad Range Protein Ladder (Thermo-Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Levels of inflammation-associated cytokines were assessed by MILLIPLEX MAP Porcine Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assay (Millipore Sigma), following the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were then blocked with 300 μL of blocking buffer (1% BSA and 10% bovine calf serum (BCS) (Sigma) in PBS at 37°C for 2 hrs ...
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bioRxiv - Genomics 2020Quote: ... of GAPDH (cytoplasmic protein) and Histone H3 (nuclear protein) using rabbit anti-GAPDH antibody (1:5000, Sigma) and rabbit anti-histone H3 antibody (1:10000 ...
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Immunology 2021Quote: ... Nunc-Immuno™ MicroWell™ 96 well ELISA plates (Millipore) were coated overnight with 50 μl per well of 2ug/ml RBD-His or Spike-His protein in carbonate buffer pH9 ...
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bioRxiv - Physiology 2020Quote: ... follicle stimulating hormone (FSH)/luteinizing hormone (LH) (ELISA, EMD Millipore), AMH (ELISA ...
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bioRxiv - Immunology 2021Quote: ... Elisa plates were coated with 100ug/ml of OVA (Sigma) prepared in a coating buffer (Na2CO3 ...
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bioRxiv - Molecular Biology 2019Quote: ... ELISA 96-well plates (Nunc-Immuno™,Sigma-Aldrich®) were coated with 200 ng of purified anti-NS1 monoclonal antibody in carbonate buffer (0.05 M ...
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bioRxiv - Immunology 2022Quote: ... 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma) was added and after 20 minutes the reaction was terminated by adding Stop Reagent for TMB Substrate (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pramlintide was quantified by ELISA (Merck Millipore, cat # EZHA-52) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Immunology 2022Quote: ... human bronchoalveloar lavage fluid and mouse serum of animals dosed with human THP were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0751) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: IDUA enzyme expression was measured fluorometrically in a 96-well plate using a Human IDUA ELISA kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s sandwich assay protocol ...
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bioRxiv - Molecular Biology 2019Quote: BJ1hTERT fibroblasts were treated with either a kinase inhibitor that prevents DNA-PKcs autophosphorylation (NU7026, Sigma) or MRE11 endonuclease activity inhibitor (PFM01 ...
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bioRxiv - Cell Biology 2023Quote: CRAF kinase activity was measured as described previously (17) and according to the manufacture’s protocol (Millipore). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Pyruvate kinase of rabbit muscle and Malate Dehydrogenase of pig heart muscle were purchased from Sigma. Protein concentrations were determined with via Bradford assay (Bio-Rad Protein Assay Dye Reagent).
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bioRxiv - Microbiology 2024Quote: ... and incubated overnight in 100ml reaction buffer (kinase buffer + 0.2mg/ml BSA (BSA Fraction V, Sigma) + 50□g/ml kanamycin) ...
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bioRxiv - Developmental Biology 2024Quote: ... the c-RAF kinase construct was induced by adding 250 nM 4OHT (4-Hydroxytamoxifen, Sigma, H7904) to the media.
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bioRxiv - Evolutionary Biology 2019Quote: ... Proteins were visualized after staining with ProteoSilver Silver Stain Kit (Sigma-Aldrich) (S.Fig.1 ...
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bioRxiv - Neuroscience 2021Quote: ... we used the EZ-Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) following the manual with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich), using BSA as a standard and aliquots maintained at −80°C until required ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using the bicinchoninic acid assay (BCA) kit (Sigma).
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bioRxiv - Genetics 2020Quote: ... Nuclear and cytoplasmic protein extracts were prepared using NXTRACT kit (Sigma-Aldrich) according manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma Alrich). Protein lysates (50 μg ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein activity was measured by an ATPase activity kit (Sigma-Aldrich) as described by the manufacturer.
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bioRxiv - Pathology 2020Quote: Dissolve proteins and FITCs in FluoroTag™ FITC Conjugation Kit(Sigma-Aldrich)kit buffer ...
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Immunology 2021Quote: RIP was performed by Magna RIP RNA binding protein immunoprecipitation kit (Millipore). A549 cells were lysed into RIP lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich). Prior to their use ...
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bioRxiv - Neuroscience 2023Quote: ... transferrin and transthyretin) using the ProteoPrep 20 plasma protein immunodepletion kit (Sigma), by performing two immunodepletion cycles ...
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bioRxiv - Biophysics 2024Quote: ... BCA Protein Assay Kit and oligonucleotides were purchased from Sigma Aldrich (international). Low melting temperature agarose was purchased from Fisher Bioreagents (Massachusetts ...
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bioRxiv - Biophysics 2020Quote: ... We induce protein expression by adding 1 mM isopropyl β-d-1-thiogalactopyranoside (Sigma) when the cell density reaches an ODλ=600nm of 0.6 ~ 0.7 ...
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bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Immunology 2024Quote: ... Viral nucleoprotein (NP) was detected by ELISA as described using a 1/1,000 dilution of the anti-NP antibody (Millipore cat. #MAB8257) and a 1/2,000 dilution of the horseradish peroxidase-labeled goat anti-mouse IgG (SeraCare cat ...
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bioRxiv - Cancer Biology 2021Quote: ... at 1:56.6 dilution and Protein-A-agarose beads (Millipore, cat# IP02 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich). Protein lysates were mixed with 5X SDS sample buffer and boiled then resolved on SDS-PAGE gels ...