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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Bioengineering 2024Quote: ... The gelatin methacryloyl (GelMA) solution was diluted at 1:4 in prewarmed 1X PBS and placed in dialysis tubes (Sigma-Aldrich, 12-14 kDa MWCO, Cat # 21-152-14). The GelMA solution was dialyzed for 3 days against 3L of 1X PBS followed by 3 days against 3L of deionized water ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Biophysics 2021Quote: HEK-ACE2 cells were seeded in 0.1 mg/mL poly-L-lysine (P9155-5MG, Sigma-Aldrich) coated 96-well plate to reach 70-80% confluency after 24 Hrs ...
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bioRxiv - Cancer Biology 2021Quote: ... and HEK-293T cells were grown in Dulbecco’s modified Eagle’s medium (Sigma-Millipore, Cat No. D5796) supplemented with penicillin/streptomycin (Corning ...
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bioRxiv - Cancer Biology 2021Quote: ... and HEK-293T cells were grown in Dulbecco’s modified Eagle’s medium (Sigma-Millipore, Cat No. D5796) supplemented with penicillin/streptomycin (Corning ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Bioengineering 2021Quote: Fixed joint samples were decalcified in 14% EDTA (pH 8.0, Sigma, Cat.# E5134) for 2–3 weeks ...
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bioRxiv - Bioengineering 2020Quote: ... This was followed by dialysis (cellulose membrane 14 kDa cut off; Sigma-Aldrich) against MQ-water at 4°C for 5-7 days ...
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bioRxiv - Bioengineering 2020Quote: ... 7 and 14 days of culture using Phosphosafe Lysis Buffer (Novagen, Madison, WI). Equal amounts of protein lysates (10-20 μg total protein ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies for immunohistochemistry are keratin 14 (KRT14) (Sigma Aldrich, SAB4501657, 1:200) cleaved caspase-3 (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... 10 mM glucose and 14 g/L oleic acid (65.0-88.0%, Sigma Aldrich) were supplemented ...
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bioRxiv - Neuroscience 2022Quote: cFos::tTA mice were administered Doxycycline (Dox, Sigma-Aldrich, CAS 24390-14-5) in the drinking water at 0.2 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 7 μl GLOX solution [14 mg glucose oxidase (Sigma Aldrich, cat. no. G2133)] ...
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bioRxiv - Cancer Biology 2022Quote: ... for 14 days or with 1 mg ml-1 puromycin (Sigma-Aldrich, #P8833) for 7 days.
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bioRxiv - Developmental Biology 2023Quote: ... a 14 h incorporation pulse of 5-ethynyl-2’-deoxycytidine (EdC; T511307, Sigma) at 2.5 µM was performed in ESCs ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as doxycycline hyclate (Cat#24390-14-5) were obtained from Sigma. DYKDDDDK Epitope Tag (Cat#NBP1-06712 for WB) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in a Labfors Bioreactor (Infors, Switzerland) in serum-free medium (Ex-Cell 293, Sigma) for 5 days ...
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bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
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bioRxiv - Immunology 2022Quote: 30µL/well of rabbit anti-His tag antibody (Sigma Aldrich, catalog #SAB5600227) was adsorbed at a concentration of 0.5 μg/mL on to 384 well microplates (Thermofisher ...
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bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Plant Biology 2024Quote: ... and confirmed by Western blotting using His-tag antibody (Sigma; SAB 1305538). The His-tagged GUN1-PS protein was produced essentially as described (19) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
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bioRxiv - Cancer Biology 2020Quote: ... constructs were transfected with packaging vectors into HEK-293T with Fugene HD (Sigma-Aldrich, St. Louis, MO). After plating ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells were maintained in DMEM10 containing 100 µg/ml G418 (Sigma Aldrich. St Louis, MO). African green monkey kidney cell line Vero E6 ATCC #CRL-1586 were cultured at 37°C in 5% CO2 in Dulbecco’s Modified Eagle’s high glucose medium (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and HEK 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; D6429; Millipore Sigma, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: Human embryonic kidney HEK-293T were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma F8067) and gentamycin (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... or HEK-ssCXCR2-tsNluc cells were washed twice with phosphate-buffered saline (PBS, Sigma-Aldrich, Pool, UK) to remove excess labelling or growth medium before being removed by scraping into 10ml PBS ...