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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were transfected with pLVX-FAP-TRF1 and Mission Packaging Mix (Sigma) to produce lentivirus ...
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bioRxiv - Biophysics 2019Quote: ... AAVs were generated in HEK 293T cells and purified by iodixanol (Optiprep, Sigma; D1556) step gradients as described (Challis et al. ...
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bioRxiv - Genomics 2021Quote: ... Both hPBMCs and HEK 293T cells were cryopreserved in FBS + 10% DMSO (Sigma-Aldrich). Prior to FACS sorting ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2019Quote: ... 1 x 105 HEK 293T cells/well were seeded onto poly-lysine (Sigma; 7405) coated 24-well plates ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK-3.x cells were detached from the flask using trypsin/EDTA (Sigma-Aldrich) and resuspended in 10 mL Leibovitz’s L-15 media supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2022Quote: Human HEK-293T cells transfected with either LacZ shRNA (control) or IKKα shRNAs (Sigma) along with their corresponding packaging plasmids were used to prepare lentiviruses from cell culture medium 48 h after transfection ...
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bioRxiv - Microbiology 2023Quote: The total RNA from Ace2-HEK-293T cells was isolated using TRI Reagent (Sigma) as per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting fragments were subsequently cloned into p3xFLAG-CMV-14 (Sigma) or pGEX-6P-1 (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... The retained phages were eluted with TEA-solution (14% trimethylamine (Sigma) pH 10 ...
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bioRxiv - Cell Biology 2020Quote: ... and 50 ng of purified active Src (Millipore-Sigma, 14-326) or PAK (Millipore-Sigma ...
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bioRxiv - Synthetic Biology 2022Quote: ... and subsequently decalcified with a 14% ethylenediaminetetraacetic acid (EDTA, SIGMA, USA) solution for four weeks ...
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bioRxiv - Microbiology 2020Quote: ... femurs were decalcified in 14% EDTA (Sigma-Aldrich, Sydney, NSW, Australia) at 37 °C for 7 days ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 14 µg/l sodium selenium (Cat. # S5261; Sigma-Aldrich), 19.4 mg/l insulin (Cat ...
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bioRxiv - Neuroscience 2019Quote: ... N--N-ethyl-2-bromobenzylamine (DSP4)14 (Sigma, 40616-75-9) ...
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bioRxiv - Cell Biology 2020Quote: ... and 50 ng of purified active Src (Millipore-Sigma, 14-326) or PAK (Millipore-Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... followed by the addition of 14 mM iodoacetamide (IAA, Sigma Aldrich) for 30 min at room temperature protected from light [25] ...
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bioRxiv - Bioengineering 2022Quote: ... 10 nM Gastrin I (1-14) (Sigma Aldrich, St. Louis, USA), 50 ng/mL recombinant human EGF ...
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bioRxiv - Microbiology 2023Quote: ... The retained phages were eluted with TEA-solution (14% trimethylamine (Sigma) pH 10 ...
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bioRxiv - Genomics 2023Quote: ... 25 μL 100 mM lithium diiodosalicylate (Millipore Sigma #653-14-5)) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 14 µg/l sodium selenium (Cat. # S5261; Sigma-Aldrich), 19.4 mg/l insulin (Cat ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Immunology 2019Quote: ... Component DNA plasmids were transfected into Lenti-X-HEK-293T cells via GeneJuice® (Novagen) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T cells were seeded onto acid-washed coverslips coated with 0.1% gelatin (Sigma-Aldrich) in 24-well plates at 5×104 cells/well ...
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bioRxiv - Cancer Biology 2022Quote: ... Human embryonic kidney HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma). Cell culture media were supplemented with 10% fetal bovine serum (FBS ...