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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ∼25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Microbiology 2023Quote: ... University of Lausanne) and Human Embryonic Kidney (HEK) 293T cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... The HEK cells used in this study were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). These cells were cotransfected with 0.1 - 1 μg of mutant hHV1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, cat. no. D6429) supplemented with 10% heat-inactivated foetal bovine serum (hiFBS ...
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bioRxiv - Immunology 2023Quote: ... HEK-293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)/F12 medium (Cat# D8437, Sigma, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to a density of 1 million cells/mL and transfected using 293 transfection free reagent (Millipore) mixed with equal ratios of heavy and light chain encoding plasmids with 250 μg of DNA per one liter of culture ...
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bioRxiv - Immunology 2020Quote: ... pTT3-SARS-CoV-2-S was transfected into 293E cells using 293 Free transfection reagent (EMD Millipore Cat # 72181) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 3) Deferoxamine mesylate salt (DFOA, Sigma Aldrich, Saint Louis, MI, USA, CAS: 138-14-7) at 1μM concentration 4 ...
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bioRxiv - Bioengineering 2020Quote: ... The proximal half of the tibia was fully decalcified in 14% EDTA (Sigma-Aldrich E5134), pH 7.4 prior to paraffin embedding ...
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bioRxiv - Microbiology 2021Quote: ... The fatty acid fraction was methylated using BF3 in methanol (14% v/v, Sigma Aldrich) at 80 °C for 2 h ...
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bioRxiv - Immunology 2022Quote: ... through the High Sensitivity T-Cell Discovery Array 14-Plex (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... 40 μg/ml glucose catalase and 14 mM MEA in H2O (all reagents from Sigma).
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bioRxiv - Neuroscience 2020Quote: ... DIV (days in vitro) 14 neurons were incubated with 5µg/ml puromycin (Sigma, Cat #P8833) for 30 min and then harvested in Buffer H (50 mM β-glycerophosphate ...
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bioRxiv - Cell Biology 2022Quote: ... GLOX solution (250 μl) consisted of 14 mg Glucose Oxidase (Sigma-Aldrich, G-2133-50ku), 50 μl Catalase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were analyzed 14 days after treatment with or without 10 mg/kg EIPA (Sigma) by i.p ...
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bioRxiv - Pathology 2020Quote: ... and on day 14 cardiomyocytes were dissociated and frozen in CS10 cryopreservation media (Sigma-Aldrich). H7 hESCs (WA07 ...
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bioRxiv - Neuroscience 2020Quote: ... 64 mg/L L- ascorbic acid and 14 μg/L sodium selenite (both from Sigma), and supplemented with 100 ng/mL FGF2 ...
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bioRxiv - Biochemistry 2022Quote: 14 out of the 46 fragments in the Halo Library were purchased from Sigma Aldrich and Alfa Aesar ...
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bioRxiv - Microbiology 2023Quote: ... were cultured in Rosewell Park Memorial Institute (RPMI) media supplemented with 14 mM NaHCO3 (Sigma), 15 mM HEPES buffer (pH 7.4),1 mM sodium pyruvate (GIBCO) ...
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bioRxiv - Neuroscience 2023Quote: DIV 14 cortical neurons plated on microfluidic chambers were treated with 2μM puromycin (Sigma, P8833) for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... The crude was purified by dialyzing (membrane cutoff = 12–14 kDa) against DMF (Sigma-Aldrich) for 24 h by changing the DMF every 8 h ...
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bioRxiv - Biochemistry 2024Quote: ... and cultured post-transfection for 48 hrs in 14 cm dishes in DMEM (Sigma-Aldrich) supplemented with 10% (vol/vol ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with anti-6x His antibody horseradish peroxidase (HRP) conjugate (Sigma A7058-1VL ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant containing 20mM imidazole was incubated with Ni-NTA His•Bind® Resin (Novagen) beads with end-to-end shaking for 4 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... the His-tag was cleaved using a Thrombin kit (Millipore Sigma, St. Louis, MO). Confirmation of the His-tag removal was confirmed by W ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were loaded on chromatographic columns filed with His-select resin (Millipore Sigma) or glutathione resin (GE healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Ni-NTA pull down was conducted by using HIS-Select Nickel Affinity Gels (Sigma). After lysing cell pellet with buffer A (6 M guanidine-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti His-tag (Cat no. H1029-5ML, Sigma, USA) as well as with anti GST antibody (Cat no ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
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bioRxiv - Neuroscience 2020Quote: ... The His-tag was removed by O/N incubation with TEV protease (Millipore Sigma) at 4 °C followed by negative INAC ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...