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Citations for Millipore Sigma :
4401 - 4450 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from bud samples using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) in accordance with the manufacturer’s protocol and treated with genomic DNAse using DNASE 70-On Column DNase I Digestion set (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: The MTT assay for cell proliferation was performed using MTT Cell Growth Assay Kit (Sigma Aldrich CT02). HEK293 cells transfected with either cmv-gfp (control ...
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bioRxiv - Immunology 2023Quote: ... and routine testing for Mycoplasma contamination was performed using the Venor GeM Mycoplasma Detection Kit (Sigma, MP0025).
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bioRxiv - Biochemistry 2024Quote: ... Cell lines were verified to be mycoplasma-free with the LookOut Mycoplasma qPCR detection kit (Sigma-Aldrich).
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated with the Spectrum Plant Total RNA Kit (Sigma-Aldrich, Saint-Louis, MO, USA) using protocol B and treated with DNase I (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed as described by the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Darmstadt, Germany) using the kit’s columns and reagents following the protocol for Gram-positive bacteria for all samples ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) as per instruction manual and triplicates were pooled and diluted 1:5 for a second PCR with Illumina adapter sequences ...
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bioRxiv - Neuroscience 2024Quote: ... AAV purity was tested by silver staining using the ProteoSilver™ Silver Stain Kit (Sigma PROTSIL1-1KT). AAV titer was determined by qPCR to normalize titers between different viral vector batches using LightCycler 480 SYBR Green I Master (Roche 04707516001) ...
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bioRxiv - Neuroscience 2024Quote: ... Linear fragments were then re-ligated using the Rapid DNA Dephosphorylation and Ligation Kit (Roche, Millipore Sigma): dephosphorylating the backbone fragment and ligating at room temperature for at least fifteen minutes.
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The subsequent steps in the proximal ligation assay were performed using the Duolink PLA Fluorescence kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Vaginal hydroxyproline content was used as a surrogate marker for collagen.10 The hydroxyproline assay kit (Sigma) was used according to manufacturer’s direction ...
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bioRxiv - Microbiology 2024Quote: The amount of β-lactamase was measured using a β-lactamase Activity Assay Kit (Sigma-Aldrich, US). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial and cytoplasmic protein was isolated using the mitochondrial/cytoplasmic fractionation kit from Millipore Sigma (cat.#: MIT1000). Primary antibodies used include the following ...
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bioRxiv - Immunology 2024Quote: ... The purity of the extracted LPS was evaluated using the total carbohydrate assay kit (MAK104, Millipore Sigma) which determined that the purity was >99.5%.
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bioRxiv - Immunology 2024Quote: ... Cell lines were confirmed to be mycoplasma-free with the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). Genotyping of various mice used in this work was accomplished with the APExBIO direct mouse genotyping kit and primers described in Table S1 ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Mycoplasma was monitored in the U937 macrophages using the Venor GeM Mycoplasma Detection Kit (Sigma-Aldrich #MP0025). Two days prior to challenge with L ...
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bioRxiv - Microbiology 2023Quote: ... LDH activity was measured using the Lactate Dehydrogenase Activity Assay Kit (Sigma-Aldrich, St. Louis, MO, USA). For histology ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was obtained from cells using the GenElute mammalian total RNA miniprep kit (Sigma, RTN350-1KT). RNA-sequencing (RNA-seq ...
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bioRxiv - Biochemistry 2024Quote: Intracellular ROS were quantified in transfected HEK293T cells using the Fluorometric Intracellular ROS Kit (Sigma-Aldrich, MAK145). Cells were imaged on a Yokogawa HCS CQ1 confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... TUNEL analysis was carried out using the ApopTag Peroxidase in situ cell apoptosis detection kit (Millipore, S7100) according to the manufacturer’s directions ...
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bioRxiv - Bioengineering 2024Quote: ... The ALP activity was measured in triplicate using an ALP assay kit (Sigma-Aldrich, St. Louis, MO) in microtiter plates ...
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bioRxiv - Immunology 2023Quote: Cytokine quantification was determined using the MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Kit (MCYTMAG-70K-PX32, Millipore). The assay was conducted as per the manufacturer’s instructions and plates were ran on a MAGPIX instrument with xPONENT software ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from barley tissues using a Spectrum™ Plant Total RNA Kit (Sigma-Aldrich). 2 μg of total RNA was purified using the TURBO DNA-fee™ Kit to remove genomic DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Proximity ligation was performed using the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) according to instruction manual ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lines were routinely tested for Mycoplasma contamination using the Venor GeM Mycoplasma Detection Kit (Sigma, MP0025).
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bioRxiv - Microbiology 2023Quote: Cell viability was determined using the XTT assay kit TOX2-1KT according to the manufacturer’s instruction (Sigma). Medium containing DMSO at 1% or less was used as vehicle control.
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bioRxiv - Microbiology 2023Quote: ... The LPS bands were visualised by silver staining using ProteoSilver kit (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Cancer Biology 2022Quote: This assay was carried out using QCM™ Gelatin Invadopodia kit (Cat. # ECM670, EMD Millipore, Billerica, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Protein—protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: The nuclei are isolated using the Nuclei EZ Prep kit (NUC101, Sigma-Aldrich, Saint Louis, Missouri, USA), as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Free and total forms of cholesterol were measured by using the Cholesterol Quantitation Kit (Sigma-Aldrich, MAK043) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... then nuclei and cytoplasmic fractions separated using CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich, NXTRACT-1KT). The resulting protein was run on a western blot and normalized to loading controls Histone H3 for nuclear fraction and alpha-tubulin for cytosolic (Sup ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated from 25 ng of RNA using the TransPlex Complete Whole Transcriptome Amplification Kit (Sigma) and 16 cycles of amplification ...
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bioRxiv - Cell Biology 2022Quote: ... ALP activity post-fracture was evaluated using a modification of the ALP Leukocyte kit (86R-1KT) (Sigma) with Fast Blue-BB salt (F3378) ...
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bioRxiv - Microbiology 2022Quote: Glucose in LB was quantified using the Glucose (GO) Assay Kit from Sigma-Aldrich (Catalog Number GAGO20) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... then 350μl RA1 lysis buffer (part of the NucleoSpin RNA Kit) and 3.5μl β-Mercapthoethanol (Sigma Aldrich) were added to the samples and then ...
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bioRxiv - Biochemistry 2023Quote: ... All cells were examined negative for mycoplasma infection using PCR-based Mycoplasma Detection Kit (Sigma, MP0035-1KT). Mouse embryonic fibroblast cell (MEF ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL staining was performed with the ApoTag Plus Fluorescein in Situ Apoptosis kit (Millipore Merck, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL staining was performed with the ApoTag Plus Fluorescein in Situ Apoptosis kit (Millipore Merck, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: PLA was performed with the Duolink® Proximity Ligation Assay kit according to the manufacturer’s instructions (Sigma). Fixation was performed as in the normal immunostaining procedure ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for the mycoplasma absence using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: β-Gal staining was performed with a β-Galactosidase Reporter Gene Staining Kit (Sigma- Aldrich, St. Louis, USA), following manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The ECM fraction was isolated from the frozen tissues using the Compartment Protein Extraction Kit (Millipore, #2145) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were extracted from residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Metabolite profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under the control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Microbiology 2023Quote: RNA-binding protein immunoprecipitation (RIP) was conducted using the Magna RIP kit (catalog no. 17-700; Millipore) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Generic fluorescent staining of EVs was performed with the PKH67 labelling kit (Sigma–Aldrich, St. Louis, USA) as previously described [21 ...
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bioRxiv - Microbiology 2023Quote: The extraction of bacterial genomic DNA was performed using the GenElute Bacterial Genomic DNA kit (Sigma Aldrich), according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... after which SA-β-gal staining was performed using a Senescence Cells Histochemical Staining Kit (Sigma, CS0030). Cells were incubated with the staining mixture overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Nuclei extractions were performed by using the Nuclei PURE Prep kit (Cat#NUC201, Sigma-Aldrich, Shanghai, China). For MNase assay ...