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Citations for Millipore Sigma :
4351 - 4400 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... following incubation using materials from the PKH26 Red Fluorescent Cell Linker Kit (Millipore Sigma, St. Louis, MO), the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... the chloroplasts were first isolated from the intact leaves using a chloroplast isolation kit (Sigma-Aldrich, USA), after which further intact chloroplasts were harvested using a 40/80% Percoll gradient ...
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bioRxiv - Physiology 2020Quote: Proximity ligation assay was performed using the Duolink® In Situ Orange Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmids were extracted and purified using the GenElute plasmid miniprep kit (Sigma-Aldrich, St. Louis, MO, USA). EcoRI and KpnI cut sites were introduced using restriction enzyme cut-site primers (Supplementary file 3 ...
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bioRxiv - Cell Biology 2021Quote: The glycerol content of cell culture media was quantified using a Glycerol Assay Kit (Sigma Aldrich MAK117) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Biochemistry 2021Quote: Protein-protein interactions were analyzed using Duolink in situ orange starter fluorescence kit (mouse/rabbit, Sigma-Aldrich) in human osteosarcoma U2OS cells (WT ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested backbone was purified from 0.7 % Agarose Gel using the GenEluteTM Gel extraction Kit (Sigma Aldrich, NA1111). Vector and miRNA target inserts were ligated at 1:10 molar ratio using 400 U of T4 DNA Ligase (NEB ...
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bioRxiv - Genetics 2020Quote: ... nuclei were enriched from the Dounce-homogenized brain tissues using Nuclei EZ prep Kit (Sigma, NUC-101). DNA were digested with micrococcal nuclease (MNase ...
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bioRxiv - Neuroscience 2021Quote: Glutamate quantity in hippocampal homogenates (10 μl) was measured colorimetrically utilizing Glutamate Assay Kit (Sigma-Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Proximity ligation assays were performed using the Duolink® flowPLA Detection Kit – Orange (Millipore Sigma, Cat. DUO94003) according to manufacturers recommended protocol with minor changes ...
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bioRxiv - Physiology 2021Quote: ... paraffin sections were dewaxed and rehydrated and stained using an Elastic Stain Kit (Millipore, HT25A, Burlington, MA). Brightfield imaging was done on a Nikon E600 microscope with Nikon NIS-Elements software ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of digoxigenin-labeled probes was performed using the DIG RNA Labeling Kit (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was extracted using the GenElute mammalian total RNA miniprep kit from Sigma (Sigma, RTN350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were routinely tested for mycoplasma contamination using the Lookout Mycoplasma PCR Detection Kit (#MP0035; Sigma-Aldrich). Cells were treated with the proteasome inhibitor MG132 (ApexBio ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was performed using manufacturer’s instructions for the EZ-Nuclear RIP Kit (EMD Millipore, #17-10 523), followed by cDNA synthesis and qRT-PCR for 5’ETS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were tested periodically for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit from Millipore Sigma.
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bioRxiv - Evolutionary Biology 2020Quote: All Digoxygenin (DIG) labeled probes were generated using the Roche DIG labeling kit (Sigma Cat no 11277073910). Antisense probes were generated using Pme1 (NEB cat no R0560S ...
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bioRxiv - Cell Biology 2020Quote: ... were constructed by the ligation-independent cloning method using a pET-41 Ek/LIC vector kit (Novagen). The coding sequences of human LC3B and GATE-16 proteins were amplified by PCR with KOD-Plus-Neo DNA polymerase (Toyobo) ...
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bioRxiv - Immunology 2020Quote: ChIP assays were performed with an EZ ChIP kit purchased from Upstate Biotechnology (Millipore Corporation Billerica, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: Deglycosylation of Bl-Eng2 was performed by using the GlycoProfile(tm) IV Chemical Deglycosylation kit (Sigma-Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... to be further randomly amplified using a modified Whole Transcriptome Amplification 2 (WTA2) kit procedure (Sigma-Aldrich). The products were purified ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA extraction was performed with a REDExtract-N-Amp Tissue PCR Kit (Sigma, St. Louis, MO), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... total protein concentration was measured in triplicates by Total Protein kit (Sigma-Aldrich, St. Luis, MO, US) using bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... paraffin-embedded tissue sections (3-mm) using commercial Masson’s trichrome staining kits (Sigma–Aldrich, St. Louis, MO) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The amplified hoc products were gel-extracted following manufacturer’s protocol (GenElute™ Gel Extraction Kit, Sigma Aldrich). Wildtype and D246N hoc genes were individually cloned in-frame to expression vector pPROEX-HTb ...
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bioRxiv - Immunology 2021Quote: ... Nuclear isolation was performed using Nuclei EZ Prep kit per manufacturer’s instructions (Sigma Aldrich, St. Louis, MO). Fractions were subsequently lysed with Triton X-100 lysis buffer (1% Triton X-100 ...
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bioRxiv - Immunology 2020Quote: ... The TUNEL assay was performed using the ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA was extracted from clonal cell lines using the GenElute Mammalian Genomic DNA kit (Sigma-Aldrich) and the K6a and K6b gene fractions where the indel mutations were expected were amplified by PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with primary antibodies (1:200) which were diluted with Immunoreaction Enhancer Kit (Millipore sigma) overnight at 4°C in a humid chamber ...
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bioRxiv - Neuroscience 2022Quote: ... DNA for experiments was prepared using either the GenElute HP Endotoxin-Free Maxi Prep Kit (Sigma, NA0410) or the EndoFree Plasmid Maxi Kit (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... and then treated with ligase from the Duolink In Situ Detection Reagents Green kit (Sigma, Cat# DUO92014) for 30 minutes in a humidity chamber at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... genomic DNA from EpiLC at D3 was isolated using the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma) with RNase treatment ...
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bioRxiv - Cell Biology 2022Quote: The extraction of RNA from MEF was performed with the GenElute Mammalian Total RNA Miniprep Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The subcellular fractionation was conducted according to the instruction of nucleocytoplasmic separation kit ( Sigma, N-3408-200ML). The nucleocytoplasmic fractions were then determined by Western blotting with the relevant antibodies.
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bioRxiv - Immunology 2022Quote: ... with protein concentration determined by Coomassie G-250 Bradford protein assay kit following manufacturer’s instructions (Sigma, UK).
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from banana organs using a Spectrum Plant Total RNA Kit (Sigma Aldrich, India) and treated with RNAse-free DNase I (Fermentas Life Sciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... Using the commercial Click-iT TUNEL (In Situ Cell Death Detection Kit, Fluorescein – Sigma-Aldrich – ref 11684795910) colorimetric detection kit ...
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bioRxiv - Plant Biology 2022Quote: ... Biotinylated DNA fragments were purified following the NucleoSpin Gel clean-up kit (Sigma-Aldrich, catalog No. 740609.50) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... ethanol concentrations were measured using the NAD-ADH Reagent kit following the manufacturer’s protocol (Sigma-Aldrich, N7160). The 340 nm spectrophotometric absorbance values were converted to mM and adjusted by the estimated 1 μL volume of an average fly to calculate ethanol concentrations.
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bioRxiv - Plant Biology 2022Quote: ... and fruit tissues were isolated using the SpectrumTM Plant total RNA kit (Sigma, USA, cat. no. STRN50). Library preparation (Illumina Stranded mRNA Prep Ligation Kit ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... A horseradish peroxidase-coupled secondary antibody was used and revealed with the Immobilon-P kit from Millipore.
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bioRxiv - Neuroscience 2024Quote: ... Proximity ligation assays were performed on fixed cultured OPs using the Duolink® PLA kit (Sigma-Aldrich). This was conducted using anti-INO80 and anti-OLIG2 antibodies along with anti-SOX10 (positive control ...
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bioRxiv - Microbiology 2024Quote: ... 5 and 7 dpi were quantified using the Milliplex MAP Porcine Cytokine/ Chemokine Magnetic bead kit (Millipore) and the BioPlex Magpix Reader (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (cat#MP0035, Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Neuronal cytotoxicity was determined with the Lactate Dehydrogenase Activity Assay Kit according to the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... Nuclei extraction and immunoprecipitation were conducted as previously described33 using CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich) and high-affinity GFP-Trap magnetic agarose (ChromoTek ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA extraction from inter-pathogen zone was carried out using an RNAeasy kit from Sigma Aldrich, and the RNA quality was evaluated using a Nanodrop spectrophotometer ND1000 ...