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Citations for Millipore Sigma :
4351 - 4400 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Proteins were then detected with monoclonal mouse anti-FLAG M2 (1:1000; Sigma-Aldrich, St. Louis, MO) and polyclonal rabbit anti-6xHis (1:2,000 ...
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bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
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bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Immunology 2022Quote: ... All lentiviral vectors (pLKO.1) expressing shRNAs used for knockdown of host proteins were purchased from Sigma.
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bioRxiv - Cancer Biology 2019Quote: ... The lyophilized proteins were resuspended in 1 ml of a 2 mg/ml DTT (1,4-Dithiothreitol, Sigma) solution and incubated at 50C for 2h ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg of extracted protein in lysis buffer was immunoprecipitated overnight with Flag-M2 affinity gel (Sigma) at 4°C ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were digested overnight at 37°C using trypsin (1:100 w/w, enzyme/protein, Sigma-Aldrich) and LysC (1:100 w/w ...
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bioRxiv - Developmental Biology 2019Quote: ... The target protein levels were normalized to the endogenous control (GAPDH, mouse, Millipore Sigma, MAB374, 1/500). The membranes were scanned on the LI-COR Odyssey CLx imaging system.
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bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).
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bioRxiv - Microbiology 2022Quote: ... followed by nucleic acid digestion with 0.5 Units of Benzonase per 1 µg of protein (Sigma-Aldrich) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant protein was removed by acidification with 5 g L-1 trichloroacetic acid (Supelco from Sigma-Aldrich), incubation for 20 min in room temperature and centrifugation at 20,238 x g for 10 min in a table-top centrifuge (Eppendorf 5424 ...
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bioRxiv - Biophysics 2022Quote: ... the expression of the protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG – Sigma) and the flask was placed at 37°C ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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bioRxiv - Neuroscience 2023Quote: ... or growth associated protein-43 (GAP43; rabbit anti-GAP43; 1:1000; #AB5220, AB_2107282; Millipore, Billerica, MA, USA). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Developmental Biology 2024Quote: ... TBX33xFLAG proteins were detected by chemo-luminescence using monoclonal M2 anti-FLAG antibodies (1:500, Sigma, F1804) in combination with donkey anti-mouse IgG-HRP antibodies (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were detected by western blotting using the following antibodies: Anti-GFP antibody (1:3000 dilution; Millipore), anti-aldolase antibody (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... 1:200) in the presence of protein A-sepharose beads (20 µl of 50% bead solution, Sigma) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Biochemistry 2022Quote: ... Collected protein was incubated 1 hour with 200 µL ANTI-FLAG-M2 affinity resin (Sigma-Aldrich; A2220) pre-washed with 3 times with1.0 mL Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was digested by incubation with benzonase (Sigma, 25 U per expected 1 mg of protein) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: The NSP3(1-110) protein was then immobilized on Anti-Flag M2 Affinity gel (Sigma Aldrich, A2220), and the cell lysates from stable cell line 293T-N ...
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bioRxiv - Molecular Biology 2024Quote: ... and in N2B27 serum-free media containing Mitogen-Activated Protein Kinase (MEK) inhibitor PD0325901 (1 μM) (Millipore), Glycogen Synthase Kinase 3 (GSK3 ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Microbiology 2020Quote: ... The initial rates of reaction were normalised to total protein content as determined using the QuantiPro BCA Assay Kit (Sigma). Control reactions without any substrate (G3P for GapA and GapN ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The MeRIP assay was carried out using the RNA-Binding Protein Immunoprecipitation Kit (Cat# 17-701, EZ-Magna, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore #17-700) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Lysates were assayed for phospho proteins using the Milliplex Cell Signaling kit (NFκB Magnetic Bead Signaling 6-plex, Millipore Sigma) for detection of NFκB pSer536 ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Microbiology 2022Quote: ... Sizes were only referenced according to the Gel Filtration Makers Kit (for protein molecular weight 29 kDa to 700 kDa, Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, #17-700) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.