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Citations for Millipore Sigma :
4301 - 4350 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and then incubated with a secondary anti-Rat IgG peroxidase-conjugated antibody (Sigma-Aldrich), diluted 1:5000 in 5% skim milk ...
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bioRxiv - Bioengineering 2021Quote: Cell lysates and exosomes including purified membrane vesicles were separated by SDS-PAGE and were then transferred to polyvinylidence fluoride membranes (Millipore, Darmstadt, Germany). The membrane was sealed with 5% non-fat milk for more than 1 h at room temperature and incubated with the desired primary antibodies overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Peprotech) and recombinant rat IFNγ (reconstituted in pH 8 sodium phosphate [10mM; Sigma-Aldrich] were diluted to the required concentrations (LTα 1μg + IFNγ 75ng per injection ...
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bioRxiv - Neuroscience 2021Quote: ... 0.25% trypsin-EDTA solution and rat tail collagen I were obtained from Sigma-Aldrich. Polybrene ...
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bioRxiv - Neuroscience 2021Quote: ... the rat dams were given access to saccharin (Sigma Life Sciences, St. Louis, Missouri) water containing either 0% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The rats were perfused first with pre-chilled Dulbecco’s phosphate-buffered saline (DPBS; Sigma), and then with 4% PFA (bought as 20% PFA from Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2020Quote: TNTPrL-P.ChR2:hM4Di rats were terminally anaesthetised with urethane (1.2-2g/kg i.p, Sigma). The spinal cord was exposed by laminectomy over T13-L3 spinal segments to allow access to the spinal cord (Leith et al. ...
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bioRxiv - Microbiology 2021Quote: Anti-HA (3F10) rat antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-CD63 (Ts63 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Rats were administered a sub-cutaneous (s.c.) injection of ketoprofen (Anafen®; Sigma Aldrich, Oakville ON ...
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bioRxiv - Neuroscience 2022Quote: ... rats received an intraperitoneal (IP) injection of desipramine hydrochloride (20 mg/kg, Sigma-Aldrich). For the surgery ...
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bioRxiv - Neuroscience 2020Quote: ... The amounts injected were as follows: 0.1 mg/kg rat α-CGRP (Sigma-Aldrich), 0.3 mg/kg PACAP-38 (Bachem) ...
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bioRxiv - Neuroscience 2021Quote: ... rats were first injected with 0.5 μl control enzyme (penicillinase, P0389-1KU, Sigma, USA) or chABC (C3667-10UN ...
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bioRxiv - Cell Biology 2022Quote: ... was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore), in EndoGro-MV medium (Merck Millipore ...
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bioRxiv - Immunology 2020Quote: 3D Collagen type I was prepared using 8 parts rat collagen type I (Sigma) and 1 part 10x DMEM and was neutralized to pH 7.0-7.5 by adding 0.5 parts 0.5N NaOH ...
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bioRxiv - Immunology 2019Quote: ... healthy rat hearts were embedded and frozen in OCT medium (Sigma-Aldrich, Gillingham, UK) and cut into 5μm sections ...
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bioRxiv - Neuroscience 2019Quote: ... rats were injected with lithium-chloride (127 mg/kg, IP, Sigma, St. Louis, MO) 24 hours prior to pilocarpine hydrochloride (50 mg/kg ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Immobilon-P transfer membrane, EMD Millipore corporation). After blocking the membranes with Blocking One (Nacalai Tesque ...
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bioRxiv - Neuroscience 2020Quote: ... the rat dams were given access to saccharin (Sigma Life Sciences, St. Louis, Missouri) water containing either 0% (v/v ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... RT-qPCR was carried out as previously described using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes [42] ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... For Benzidine staining of naked mole-rat methylcellulose assays a 0.2% benzidine dihydrochloride (Sigma) solution in 0.5M acetic acid was prepared ...
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bioRxiv - Microbiology 2020Quote: Antibodies were used at the following dilutions: rat anti-HA IgG1 (clone 3F10; Sigma) at 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were intraperitoneally injected with BrdU (150 mg/kg of body weight, Sigma # B5002) once a day for five consecutive days ...
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bioRxiv - Biophysics 2022Quote: ... The coding sequence for rat Munc18-3 was cloned into pET-15a (Novagen, TX) and codon-optimized for protein expression in bacteria ...
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bioRxiv - Biophysics 2022Quote: ... with the following secondary antibodies: (Fig 3,4) Goat anti-rat CF 568 (Sigma SAb4600086) 1:500 ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of anti-rat secondary antibody conjugated to horseradish peroxidase (A9037, Sigma-Aldrich) diluted 1:1000 in 5% MPBS were added to each well and incubated for 1.5 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Unilaterally-lesioned rats were injected with D-amphetamine (5.0 mg/kg, i.p.; Sigma-Aldrich) to induce dopamine release from dopaminergic neurons projecting to the striatum and were then placed in a plexiglass cylinder (38 cm diameter × 38 cm height) ...
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bioRxiv - Neuroscience 2022Quote: ... Nine-week old rats were injected with DFP (0.5 mg/kg, S.C.; D0879, Sigma) or vehicle (ice-cold PBS ...
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bioRxiv - Bioengineering 2023Quote: ... RBM-II was produced by strain HK-II in BE medium (33) and was collected on a membrane filter (0.1 μm, OmniporeFM membrane filter; Merck Millipore, Burlington, MA, USA) using a suction pump ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were used at the following dilutions: Rat anti-HA IgG1 (clone 3F10; Sigma) at 1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... TBS-T was used for washing the membranes. Membranes were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).
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bioRxiv - Neuroscience 2023Quote: Rats were anesthetised using urethane (1.5 g/kg body weight; Sigma-Aldrich; MA, USA) administered via intraperitoneal injection ...
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bioRxiv - Developmental Biology 2023Quote: Olfactory bulb sections containing rat glomeruli were treated with glycine (50 mM, Sigma-Aldrich) and ammonium chloride (50 mM ...
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bioRxiv - Genomics 2023Quote: ... The stainings were quenched with the addition of 50 ul Rat Serum (Sigma, R9759) and incubation at 4°C for 10 minutes protected from light ...
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bioRxiv - Biophysics 2023Quote: The rat cardiomyoblast cell-line H9c2 (cells derived from embryonic heart tissue; Sigma Aldrich) was genetically modified using retrovirus to achieve a stable expression of tandem tagged (mCherry-EGFP ...
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bioRxiv - Biochemistry 2023Quote: ... TR from rat liver (TrxB) was obtained from Sigma-Aldrich (St. Louis, MO, USA). pGEX-4T-1 cells were obtained from GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... The secondary antibody was rabbit anti-rat IgG conjugated to peroxidase (Sigma-Aldrich A5795), used at a 1:8000 dilution in TBS-T with 2.5% dried milk buffer for one hour ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown to 70% confluency before induction of protein expression using 1 μg/ml tetracycline (Sigma), and media supplementation with 50 μM biotin for protein labeling ...
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bioRxiv - Cell Biology 2020Quote: ... expression of EGFP fusion proteins was induced by addition of 1 μg/mL doxycycline (Sigma-Aldrich, D9891) and EGFP expressing cells were positively selected by FACS using a SONY MA900 to sort into media lacking doxycycline ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg protein was incubated with 50 μl equilibrated anti-FLAG M2 affinity gel (Sigma–Aldrich, A2220) on a rotating wheel for 1 h at 4 °C ...
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bioRxiv - Physiology 2019Quote: ... GST-free eluates of protein were prepared by using 1 unit of biotinylated thrombin (Millipore 69672-3) per 100 μl of beads and letting samples incubate on a rotator at 4° C overnight ...
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bioRxiv - Biochemistry 2019Quote: ... the buffer matrix for the protein standards was changed by adding 1% BSA (A7030, Sigma Life Science) to achieve a higher matrix complexity ...
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bioRxiv - Cell Biology 2021Quote: ... Mif2-TAF and Flag-Cse4 were detected by immunoblotting with anti-protein A (Sigma, P3775, 1:10,000) and anti-Flag (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
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bioRxiv - Systems Biology 2021Quote: ... at a 1:50 protease/protein ratio overnight at 37 °C in 100 mM NH4HCO3 (Sigma-Aldrich). The next day ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total protein synthesis was measured by immunoblot using the anti-puromycin antibody at 1:500 (EMD Millipore). Protein synthesis was quantified by densitometry using ImageJ and normalized by immunoblot using antibody specific to steady-state levels of eIF2α protein (13).
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated by SDS-PAGE and probed with antibodies against zDHHC5 (1:1,000; Sigma Prestige, HPA014670), HA (1:1,000 ...