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Citations for Millipore Sigma :
4251 - 4300 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Protein concentrations were determined using the BCA protein assay (Sigma-Aldrich) using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2022Quote: ... Thirty microliters of protein G/protein A agarose beads (EMD Millipore) were washed with lysis buffer (containing protein inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration was determined by a BCA protein assay (Sigma). A total of 50 µg protein extract was mixed with 5× Laemmli loading buffer (50% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 50 μg were resolved on an SDS/PAGE denaturing gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 60 μg were resolved on an SDS/PAGE gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were boiled and loaded on a 12% SDS-polyacrylamide gel and blotted onto a PVDF membrane (Immobilon®-P transfer membrane, Millipore). The blots were blocked for 1 h in PBS containing 0.05% Tween-20 and 3% BSA at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: The preparation of the cell membrane fraction including inner and outer membrane anchoring enzymes was modified based on the procedures described from Millipore Sigma (https://www.sigmaaldrich.com/technical-documents/protocols/biology/purifying-challenging-proteins/cell-disruption-and-membrane-preparation.html) ...
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bioRxiv - Microbiology 2020Quote: ... A total of 0.1 mg mucin per dot were immobilized by dot blotting onto an immobilon-P PVDF membranes (Immobilon P membranes, 0.45 μm, Millipore, Billerica, MA). RiGH98 was added to one dot to 1.5 μM in 50 μL and incubated for 1 h and 4 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was crosslinked to the membrane with a UVA Stratalinker 2400 and membranes were then soaked in PerfectHyb Plus (Sigma Aldrich) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Each spinal cord slice was adhered to a small piece of filter membrane (Whatman Nuclepore Track-Etched Membrane, Sigma-Aldrich, WHA110657) for convenient handling ...
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bioRxiv - Developmental Biology 2023Quote: ... 95°C and run on 10% SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham Protran Western blotting membranes – Sigma Aldrich) to validate protein quality and specificity.
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bioRxiv - Cell Biology 2022Quote: 96-well ELISA plates were coated overnight at 4°C with anti-mouse total β1-integrin (Millipore) or anti-mouse β1-integrin ...
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bioRxiv - Physiology 2021Quote: ... Total and high-molecular weight adiponectin was measured by ELISA (EZMADP-60K; EMD Millipore and MBS028367; MyBiosource).
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bioRxiv - Neuroscience 2023Quote: ... basal plasma (collection prior to IPGTT) leptin was measured using a leptin ELISA (Millipore Sigma; #EZRL-83K) following manufacturer’s instructions in duplicate ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... For the time course shown in Fig 2B 19 μl of this protein were combined with 1 μl of trypsin (Sigma; 1 mg/ml) and the mixture was incubated at RT ...
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bioRxiv - Systems Biology 2020Quote: ... on day 0 with a 50 µl volume of Escherichia coli O111:B4 LPS purified by ion exchange and with <1% protein and <1% RNA (Sigma Aldrich; catalog L3024), which was diluted in sterile ...
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bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated overnight at 4°C with 1:2000 dilution of primary antibodies (FLAG; Sigma, F7425 and HA; Invitrogen, 71-5500) followed by incubation with 1:5000 dilution of HRP-coupled secondary antibodies for 2h at room temperature and detected using enhanced chemiluminescence (Pierce ...
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bioRxiv - Microbiology 2020Quote: ... membranes were blocked as described above and β-actin was detected using mouse β-acting specific monoclonal antibody (Sigma, 1:10,000 dilution) and peroxidase-conjugated goat anti-mouse IgG (Jackson Immunolabs ...
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bioRxiv - Microbiology 2019Quote: ... after 3 days of growth membranes/biofilms were transferred to fresh agar wells containing 20uL of DNase 1 (2 Kunitz units / µL) (Sigma-Aldrich #D4263) spotted directly underneath the biofilm ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10,000; anti-rabbit, Sigma-Aldrich A0545 or anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibody 1:12000 mouse anti-α-Tubulin antibody (SIGMA, Cat. # T5168). Followed by 1h incubation with secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
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bioRxiv - Molecular Biology 2019Quote: ... For myosin heavy chain, the membranes were incubated with 1:500 dilution of monoclonal anti-myosin (skeletal, fast) (M4276, Sigma-Aldrich, USA) and monoclonal anti-myosin (skeletal ...
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bioRxiv - Immunology 2021Quote: ... Lysates were separated by SDS-PAGE using Tris-Glycine gels loaded with about 1×106 cell equivalents per well and transferred onto PDVF membrane (Millipore, Burlington, MA). Blots were blocked in either 5% Milk (Nestle ...
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bioRxiv - Molecular Biology 2022Quote: ... in Spectra/PorTM 1 RC Dialysis Membrane Tubing (SpectrumTM 132650) and concentrated in Amicon ®Ultra-2 mL centrifugal filters (Millipore UFC205024).
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of the extracted protein were electrophoresed in a 15% SDS polyacrylamide gel for 1 hour at 150V and transferred to a PVDF membrane (Millipore, MA, USA) for 1 hour at 72V ...
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... skim milk/TBS and the membrane was washed 5x with 1x TBS before incubation with streptavidin-conjugated alkaline phosphatase (dilution 1:1,000, Sigma-Aldrich; Taufkirchen, DE) in 5% (w/v ...
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bioRxiv - Plant Biology 2022Quote: ... a drop of pollen germination media was covered with a 1×1cm piece of dialysis membrane (Sigma-Aldrich catalog number, D9652-100FT). Fresh ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich, used at 1/ 1000 dilution). The antibodies were detected by using Lumi-Light Western Blotting Substrate (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day membranes were washed 3-times with TBST for 7 min and incubated with corresponding solutions of anti-mouse (A9044, Sigma; 1:15,000) or anti-rabbit (A9169 ...
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bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
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bioRxiv - Microbiology 2023Quote: ... 700 µL supernatant was transferred to a new 1.5-mL tube and concentrated using Amicon Ultra 1 mL 10K membrane filters (Millipore, catalog number: UFC501096) to ≈40 µL by centrifuging at room temperature at 14,000 x g for ≈45 min ...
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bioRxiv - Neuroscience 2023Quote: ... Four small insertions were made and the retina was flat mounted on a filter paper annulus (1 mm inner radius; 0.8 µm hydrophilic MCE MF-MilliporeTM membrane filter, Merck Millipore Ltd., Tullagreen, Ireland). The retina was then mounted photoreceptor cell side up on a perforated 60-electrode MEA chip (60pMEA200/30iR-Ti ...
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bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
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bioRxiv - Microbiology 2023Quote: ... skim milk/TBS and the membrane was washed 5x with 1x TBS before incubation with streptavidin-conjugated alkaline phosphatase (dilution 1:1,000, Sigma-Aldrich; Taufkirchen, DE) in 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... gentle peristaltic filtration (30 ml•min-1) was used to collect all cells and particles on a 0.22 μm Sterivex filter (PES membrane, Millipore, Burlington, MA, USA). Microbial biomass on the Steviex filter was used for 16S rRNA gene amplicon microbial community diversity analysis ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation (1 hr, 20°C) in TBST containing skimmed milk or bovine serum albumin (5%, Sigma, Cat# A9418). TBST (Tris-buffered saline with Tween ...
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bioRxiv - Developmental Biology 2023Quote: ... and blotting on a polyvinylidene difluoride (PVDF) membrane probed with rabbit monoclonal anti-histone H3 (Cat# 07690, EMD Millipore, 1:25000-50000), mouse monoclonal anti-alpha tubulin (Cat# NB100-690 ...
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bioRxiv - Microbiology 2020Quote: ... the corresponding drinking water matrix was filtered using sterile bottle top filter units and membrane filters (Whatman® Nucleopore™Track-Etched Membranes, 47 mm, 0.2 μm, Sigma Aldrich). New material was cleaned and stacked ...
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bioRxiv - Microbiology 2019Quote: ... and the recovery plate containing biofilm-recovered bacteria was covered with a breathable sealing membrane (Breathe-Easy® sealing membrane, Sigma, Australia), and incubated at 37 °C for 20 hours with shaking in microtiter plate reader (BMG ...
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bioRxiv - Bioengineering 2021Quote: Cell lysates and exosomes including purified membrane vesicles were separated by SDS-PAGE and were then transferred to polyvinylidence fluoride membranes (Millipore, Darmstadt, Germany). The membrane was sealed with 5% non-fat milk for more than 1 h at room temperature and incubated with the desired primary antibodies overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Immobilon-P transfer membrane, EMD Millipore corporation). After blocking the membranes with Blocking One (Nacalai Tesque ...
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bioRxiv - Cell Biology 2023Quote: ... TBS-T was used for washing the membranes. Membranes were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).