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Citations for Millipore Sigma :
4301 - 4350 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Brain proteins were extracted using RIPA Buffer (Sigma-Aldrich) containing protease inhibitors (cOmplete Tablets ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were transferred to a PVDF membrane (Millipore) using a semi-dry transfer apparatus at 10V for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Genomics 2023Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) at 95°C for 10 min and alkylated for 30 min with 40 mM iodoacetamide (Pierce) ...
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bioRxiv - Immunology 2023Quote: ... filtered through 0.45 μm low- protein-binding filters (Millipore) and concentrated via 16 hours centrifugation at 3000 x g at 10 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated using centrifugal spin concentrators (Millipore) with an MWCO of 10 kDa ...
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bioRxiv - Microbiology 2023Quote: ... Protein was estimated using BCA kit (Sigma Aldrich, USA) and 20 µg protein was loaded on 12% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 10 μM DTT (Sigma-Aldrich), alkylated with 50 μM iodoacetamide (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Ezview Red Protein G Affinity Gel (Sigma-Aldrich, E3403) was used for negative control samples ...
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bioRxiv - Biochemistry 2023Quote: ... 15 mM NEM and a protein inhibitor cocktail (Sigma)) ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred to Immobilon-FL PVDF membrane (Millipore) using 1× Power Blotter 1-Step Transfer Buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G Sepharose 4B fast flow (P3296, Sigma Aldrich) was used for the Co-immunoprecipitation and ChIP assay ...
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bioRxiv - Cell Biology 2023Quote: Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using a wet blot transfer system ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was expressed using a pETBlue-1 (Novagen) vector in E ...
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bioRxiv - Biophysics 2024Quote: Horse Myoglobin protein was purchased from Sigma Aldrich (M1882) and 22.6 mg was resuspended with 700 µL of NMR buffer (20 mM MES pH 6.2 buffer ...
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bioRxiv - Cancer Biology 2024Quote: Proteins were first reduced with 10 mM dithiothreitol (Sigma) for 1 hour at 56°C and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Sigma) and non-specific binding was blocked with 5% milk powder or 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured with Bradford reagent (Sigma-Aldrich), and 5 mg of the worm lysate was added to anti-Rabbit IgG magnetic Dynabeads (Fisher ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra filters (Millipore) to a concentration of 60 mg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on polyvinylidene (PVDF) (Sigma #P2938) or nitrocellulose (Amersham ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to Immobilon FL PVDF membranes (Millipore), and membranes were blocked using Intercept Blocking buffer (Li-Cor) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in 10 mM Tris HCl ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-myelin basic protein (MBP, Millipore, MAB386, RRID:AB_94975), rabbit anti-laminin (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Protein G Sepharose 4B fast flow (P3296, Sigma Aldrich) was used for the Co-immunoprecipitation and ChIP assay ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signals were developed and detected by chemiluminescence (Millipore) on a ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Cell Biology 2024Quote: Total cell protein was extracted using RIPA buffer (Sigma) supplemented with protease inhibitors (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and one Roche Complete protein inhibitor tablet (Millipore, 11873580001). Cells were then incubated with activated concanavalin-A beads (Bang Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were transferred to PVDF membranes (Merck Millipore), and Ponceau S (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined using the Bradford assay (Sigma), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and protein G-sepharose CL-4B beads (Sigma-Aldrich). After SDS-PAGE and immunoblotting ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were blotted onto Immobilon-FL PVDF membranes (Millipore), which was subsequently blocked for 1 hour at room temperature using a blocking buffer consisting of 1 part Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated with Amicon Ultra-15 (Millipore, USA) to a final concentration 9 mg/ml.
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bioRxiv - Plant Biology 2024Quote: ... Protein quantity was measured using Bradford method (Sigma-Aldrich). Proteins were stored in aliquots at -80°C until use.
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bioRxiv - Cell Biology 2024Quote: Protein extracts were prepared in RIPA buffer (R0278, Sigma) with protease inhibitor 1X (ab270055 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were blotted onto Polyvinylidene difluoride (PVDF) membranes (Millipore) using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were wet transferred onto a PVDF membrane (Millipore) and assessed for successful transfer with Ponceau Red (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein expression was induced with 0.2 mM IPTG (Sigma) final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were mixed with DTT (#D9779-10G, Sigma) and loading sample buffer (#NP0007 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto PVDF membranes (#IEVH85R, Millipore, USA) using the wet transfer system (#1703930 ...
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bioRxiv - Immunology 2024Quote: ... A protein A-Sepharose column (Sigma #ge17-1279-03) was used for mAb purification ...
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bioRxiv - Microbiology 2024Quote: ... We also employed SYPRO Orange Protein stain (Millipore Sigma), a protein dye ...
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bioRxiv - Cancer Biology 2021Quote: ... stained with a 1:40 dilution of anti-human IgG-FITC (Sigma, Mendota Heights, MN) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2021Quote: ... An additional plate was coated with goat anti-human IgG (Sigma, cat. no. I2136-1ML) at 4 μg/ml and IgM (Sigma ...
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Cell Biology 2020Quote: Human HeLa and HEK293T cell lines (obtained from ATCC) were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (heat-inactivated ...
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bioRxiv - Molecular Biology 2021Quote: ... 2Dorganoids were seeded in chambers pre-coated with 50 μg/ml human collagen IV (Millipore) for 2 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170, Sigma-Aldrich, USA) respectively ...