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Citations for Millipore Sigma :
4251 - 4300 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using a wet blot transfer system ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were dimethylated by adding 30 mM formaldehyde (Sigma) and 30 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using enhanced chemiluminescent detection kit (Millipore). To detect changes in the protein glycosylation ...
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bioRxiv - Biochemistry 2023Quote: ... After a preclearing step with Protein G Sepharose (Sigma), equal amounts of proteins were then incubated with anti-Flag Beads (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined by Bradford assay (Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 10 mM dithioerythritol (Merck-Millipore) for 1 h at 56°C followed by a washing-drying step as described above ...
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bioRxiv - Immunology 2023Quote: Protein samples were boiled in Laemmli buffer (Sigma-Aldrich) at 95°C for 5 min and separated on a 12% Bis-Tris gel (NuPAGE ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to Nitrocellulose membranes (GE10600012, Sigma-Aldrich). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was confirmed using Ponceau S (Sigma-Aldrich), and blots were cut into two at relevant molecular weights as needed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein constructs were expressed in BL21 (DE3) cells (Novagen), in 2xYT media ...
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bioRxiv - Neuroscience 2023Quote: ... Protein quantification was determined by Bradford reagent (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Protein was then transferred to a PVDF membrane (Millipore) overnight with NuPAGE transfer buffer ThermoFisher ...
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bioRxiv - Pathology 2023Quote: ... and separated proteins were transferred on PVDF membrane (Millipore). After blocking aspecific signal ...
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were quantified by Bradford reagent (Sigma) and purity was analysed by SDS-PAGE ...
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bioRxiv - Pathology 2023Quote: ... and separated proteins were transferred on PVDF membrane (Millipore). After blocking aspecific signal ...
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bioRxiv - Cell Biology 2023Quote: ... immunoreactive proteins were detected using Immobilon Western Kit (Millipore). Protein bands from each blot were observed by an imaging system (ChemiDoc XRS+ ...
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bioRxiv - Plant Biology 2023Quote: ... separated proteins were transferred to Immobilon-P membrane (Millipore). Protein transferred membrane was dried and washed with methanol to remove extra Coomassie blue before western blot analysis ...
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bioRxiv - Bioengineering 2023Quote: ... The proteins used were β-Lactoglobulin (βLG) (Sigma – L3908) and poly(L-Lysine ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred into a PVDF membrane (Sigma) by wet transfer at 100V for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were concentrated using Amicon Ultracel 10K filters (Millipore) and absorbance at 280 nm was measured using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were concentrated using Amicon Ultracel 10K filters (Millipore) and absorbance at 280 nm was measured using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... A protein mix standard (Sigma Aldrich, St. Louis, MI) containing thyroglobulin bovine (640 kDa) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were wet transferred onto a PDVF membrane (Millipore) and assessed for successful transfer with Ponceau Red (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... 40 μl of protein A beads per sample (Sigma; equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were prepared using RIPA buffer (Merck/Millipore). SDS-PAGE and western blotting were performed using standard protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... separated proteins were transferred to polyvinylidene difluoride (PVDF, Millipore) membranes ...
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bioRxiv - Genetics 2023Quote: ... Proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: Proteins were isolated in RIPA buffer (Sigma, Cat# R0278) with protease inhibitor cocktail (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined using the Bradford Reagent (Sigma) and the BioMate™ 3S spectrophotometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested with trypsin (Sigma Corporation, Kanagawa, Japan) at a 1:10 enzyme to substrate ratio for 2 hours at 47°C ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinylidene fluoride membranes (Millipore #IPFL00010). Membranes were blocked in blocking buffer (Rockland #MB-070 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined using Coomassie reagent (Sigma-Aldrich). Samples were denatured in LDS sample reducing buffer (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... Model proteins lysozyme from chicken egg (Sigma-Aldrich, UK) and bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... protein was concentrated using a 30K-cutoff concentrator (Millipore) and purified by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes (0.45 μm, Millipore) at 30 V ...
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bioRxiv - Immunology 2023Quote: ... which were primed with carrier protein OVA (Catalog:A5503, Sigma) 30 days before ...
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bioRxiv - Immunology 2023Quote: ... the extracted proteins were alkylated using 20mM chloroacetamide (Sigma) and incubated 30 min at RT in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were transferred to PVDF membranes (Millipore). After being blocked with 5% nonfat dry milk ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred to PVDF membranes (Millipore) according to standard protocols ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were transferred to a PVDF membrane (Millipore) using a semi-dry transfer apparatus at 10V for 30 minutes at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Actin protein was probed (monoclonal antibody from Sigma-Aldrich) as a control to verify equivalent protein loading between the variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was expressed using a pETBlue-1 (Novagen) vector in E ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were blotted using nitrocellulose membrane (Sigma Aldrich). Rabbit monoclonal halo tag antibody was used in the (1:5000 ...
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bioRxiv - Biophysics 2024Quote: ... the proteins were transferred onto a PVDF membrane (Millipore). This membrane was then washed with blocking buffer (LI-COR Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were digested with proteinase K (Cat#: P2308, Sigma), and DNA was phenol/chloroform-extracted and precipitated by 100% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... Protein was estimated using BCA kit (Sigma Aldrich, USA) and 20 µg protein was loaded on 12% SDS-PAGE gel ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated using centrifugal spin concentrators (Millipore) with an MWCO of 10 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... Brain proteins were extracted using RIPA Buffer (Sigma-Aldrich) containing protease inhibitors (cOmplete Tablets ...