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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) final concentration at 95°C for 10 minutes and alkylated with iodoacetamide (Pierce,40 mM final 30 minutes) ...
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bioRxiv - Neuroscience 2022Quote: ... 30ul of equilibrated Protein G – agarose beads (Sigma 15920010) were added to the lysates and incubated further for 3 hours.
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bioRxiv - Neuroscience 2022Quote: ... and proteins were extracted in RIPA buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitors (Pierce Protease and Phosphatase Inhibitor Mini Tablets ...
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bioRxiv - Neuroscience 2022Quote: ... myelin basic protein (MBP; 1:200; MAB386; Milipore Sigma), Synaptophysin (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein synthesis inhibitor cycloheximide was purchased from Sigma-Aldrich. USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was concentrated using Amicon Ultracel-30K (Millipore) centrifuge filters and subsequently applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was digested with sequencing grade trypsin (Sigma; #T6567) and sequencing grade LysC (Wako ...
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bioRxiv - Systems Biology 2022Quote: ... The protein was transferred to PVDF membranes (Millipore; #IPFL00010) in NuPAGE Transfer Buffer at 20 Vfor 1 h at room temperature and blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred to methanol activated PVDF (Millipore) membranes (350 mA for 60 minutes) ...
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bioRxiv - Physiology 2024Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Microbiology 2024Quote: ... Protein transfer to a PVDF-FL membrane (Millipore, #05317) was achieved at a current of 2.5 mA/cm2 for one hour ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were blotted using nitrocellulose membrane (Sigma Aldrich). Rabbit monoclonal halo tag antibody was used in the (1:5000 ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
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bioRxiv - Biophysics 2024Quote: ... the proteins were transferred onto a PVDF membrane (Millipore). This membrane was then washed with blocking buffer (LI-COR Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were digested with proteinase K (Cat#: P2308, Sigma), and DNA was phenol/chloroform-extracted and precipitated by 100% ethanol ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto a PVDF membrane (Millipore), and the membrane was blocked with blocking buffer (TBST + 5% non-fat dry milk ...
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bioRxiv - Molecular Biology 2024Quote: ... A mixture of Protein A agarose (Millipore, 16-157) and Protein G agarose (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich) for 30 min at 37 °C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto PVDF membrane (EMD Millipore IPVH00010) for 2.5 hours at 200 mA in ice-cold transfer buffer (25 mM TrisHCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to PVDF membranes (Merk Millipore) and probed with the appropriate indicated antibodies.
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the BCA kit (Sigma), except for insoluble fractions due to due to formic acid’s denaturation effect ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by Bradford assay (Sigma, #B6916), after which samples were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were blotted onto a nylon membrane (Millipore). Blots were blocked using 5% nonfat dry milk (NFDM ...
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bioRxiv - Physiology 2023Quote: ... The protein was transferred to PVDF membranes (Millipore; #IPFL00010) in NuPAGE Transfer Buffer at 20 V for 1 h at room temperature and blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were concentrated using Amicon Ultra-15 (Millipore) tubes before loading to a Superdex 75 column ...
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bioRxiv - Immunology 2023Quote: ... and proteins were transferred to polyvinylidene difluoride membranes (Millipore). Membranes were incubated overnight at 4°C with the specific primary antibodies anti-αSMA and anti-tubulin (Supplementary table 2) ...
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bioRxiv - Biochemistry 2024Quote: ... protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chlorophorm phase separation ...
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bioRxiv - Genetics 2024Quote: ... Co-purifying proteins were imaged with anti-HA (Sigma) and anti-VSV (Bethyl Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were transferred to Immobilon-FL (Millipore IPFL00010).
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bioRxiv - Biochemistry 2024Quote: ... proteins were expressed in BL21 (DE3)T1R cells (Sigma) at 18 °C overnight after induction with 1 mM IPTG ...
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bioRxiv - Biophysics 2024Quote: ... the proteins were concentrated using 30kDa MWCO Amicon (Millipore) ultra-centrifugal filters to 10 mg/mL and immediately injected in gel filtration column S200 10/300 (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein G beads (Millipore, Cat. #16-266) to the immuno-complex for 2h ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µL of the Protein G agarose bead (Millipore) slurry was washed and added to the antibody-lysate mixture and incubated for 2 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated in a 3 kDa concentrator (Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a Immobilon PVDF membrane (Millipore), which was then blocked in 4% milk in tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were immunoprecipitated using anti-Flag (M2, Sigma), anti-Myc (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... levels were normalized to total protein levels (Sigma-Aldrich, #E3138 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto a Immobilon PVDF membrane (Millipore), which was then blocked in 4% milk in tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Samples were precleared with Protein A agarose beads (Millipore), immunoprecipitated with anti-REV-ERBα (NRIDI ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were reduced in 10mM dithiothreitol (Sigma Aldrich, UK) for 30mins at 37ÅãC and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred to a PVDF membrane (Merck Millipore) after which the membrane was blocked using Odyssey Blocking buffer (LI-COR ...
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bioRxiv - Genomics 2022Quote: ... bound to Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Co-purification was investigated by western blot with an anti-GFP antibody (Roche #11814460001 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were transferred to PVDF-FL membranes (Millipore) as previously published (Michele et al. ...
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bioRxiv - Biophysics 2022Quote: All Proteins and fluorophores were procured from Sigma-Aldrich and concentration was measured using Shimazdu uv-1900 spectrophotometer ...