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Citations for Millipore Sigma :
4301 - 4350 of 4506 citations for Recombinant Human CD274 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...
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bioRxiv - Cell Biology 2020Quote: Eggs were collected from female Xenopus laevis frogs 16-18 hours after egg laying was induced by injection of 500 U of human chorionic gonadotropin (Chorulon from Merck, Sigma #CG10-10VL). Cytostatic factor-(CSF- ...
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bioRxiv - Cell Biology 2020Quote: ... Serum-free DMEM was then discarded and cells were incubated in serum-free DMEM containing 120 μg/mL human HDL (Sigma-Aldrich, L1567) for 6 h ...
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bioRxiv - Immunology 2021Quote: ... fresh living donor biopsies or cells remaining following 10X cell capture for sequencing were resuspended in 90% human serum (Sigma, cat# H4522) and 10% DMSO for cryopreservation and cooled to -80°C in a Mr.Frosty (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for immunostaining: anti-CREST/anti-centromere antibodies (human, Antibodies Inc.; 1:100) anti-Tubulin (mouse monoclonal, Sigma; 1:5000). DNA was stained with 0.5 µg/ml DAPI (Serva ...
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bioRxiv - Bioengineering 2019Quote: ... human skeletal muscle samples were minced and then dissociated into a single cell slurry with Clostridium histolyticum collagenase (Sigma, 630 U/mL) and dispase (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were blocked for 30 minutes with mouse serum and immunostained with directly Cy3-conjugated anti-human HNA (Millipore, MAB1281C3, 1:100). Nuclei were counterstained using DAPI ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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bioRxiv - Bioengineering 2019Quote: ... were maintained as monolayer cultures in flasks pre-coated with 60 μg/mL solution of human placental collagen type IV (Sigma Aldrich, MO) and grown in bronchial epithelial growth medium (BEGM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we grew PAO1-pvdSi in iron-limited CAA medium (using 100 mg ml−1 human-apo transferrin to bind iron and 20mM NaHCO3 as cofactor, both from Sigma-Aldrich, Switzerland), supplemented with various amounts of purified pyoverdine (pyoverdine concentration in μM ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for the analysis of human synovium with microscopy: rabbit polyclonal anti-CD3 (Sigma-Aldrich Cat# C7930, RRID:AB_259074), anti-CD103 (Clone EP206 ...
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bioRxiv - Bioengineering 2021Quote: ... for 12 days in SSC expansion media with the following additions: 100 ng/mL follicle stimulating hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Pathology 2021Quote: HIF-1α protein expression was depleted in human U937 cell line (ATCC, USA) using shRNA targeting HIF-1α (TCRN0000010819, Sigma Aldrich, USA) using a liposome mediated delivery (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... which was un-exposed European pooled serum.23 Secondary detection was with Human IgG (H&L) Antibody Peroxidase Conjugated (Sigma – Aldrich, Germany), developed with Pierce™ TMB Substrate Kit (Thermo Fischer ...
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bioRxiv - Neuroscience 2020Quote: ... and pre-absorption of mouse anti cathepsin-S with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO) did not result in detectable immunolabeling signal.
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bioRxiv - Microbiology 2020Quote: ... Parasites were washed thrice for 15 min in PBSTx and incubated for 24 hrs in 1:2000 Fab specific FITC conjugated goat anti human IgG (F5512, Sigma-Aldrich UK) diluted in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cell Biology 2021Quote: ... media was changed to serum-free media (DMEM, 2mM L-glutamine) overnight followed by insulin stimulation (100nM human insulin, Sigma-Aldrich, I9278), with 30-minute vehicle ...
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bioRxiv - Neuroscience 2020Quote: ... and COs derived from H9 hESCs and iPSCs were measured using the Human Oxidative Phosphorylation Magnetic Bead Panel Milliplex MAP Kit (Millipore–H0XPSMAG-16K), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and Human Kidney Cells (HKC) (acquired from Collaborator Dr. Kim Rathmell Lab, Vanderbilt University, TN) were maintained in DMEM (Sigma-Aldrich, # D6429) media supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... human muscarinic 1,2,3,4, and 5 acetylcholine receptors (M1R, M2R, M3R, M4R, and M5R) with acetylcholine (ACh; Sigma-Aldrich A6625, in measuring buffer), murine μ-opioid receptor (MOP ...
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bioRxiv - Cell Biology 2021Quote: ... Insulin secreted from the islet grafts was measured in the mouse serum three weeks post-transplant using a human insulin ELISA (Millipore, EZHI-14K).
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bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The inner surface of Transwell inserts (3.0 μm pore transparent polyester membrane) pre-coated with 100 μl of human collagen IV solution (34 μg/ml; Sigma-Aldrich, USA) were seeded with 100 μl of an enteroid fragment suspension ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were washed five times and incubated for 1 h with 1 µg/mL HRP-conjugated goat anti- human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Microbiology 2022Quote: ... 5 mL of whole human blood was layered onto 5 mL of Histopaque-1077 Hybri-MaxTM (Sigma Life Science, H8889-100 mL) in a column ...
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bioRxiv - Evolutionary Biology 2019Quote: HSA-AAG containing medium is RPMI 1640 growth medium with the addition of 341 mM HSA (human serum albumin, Sigma, Cat # A9511) and 1 mg/ml AAG (human a1-acid glycoprotein ...
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bioRxiv - Immunology 2019Quote: ... for 6 hours and then exposed to 0.5 × 105 red and 0.5 × 105 blue opsinised (1 hour in 10%v/v human serum) 3 μm diameter latex polystyrene beads (Sigma-Aldrich, Gilligham, UK) such that there were 1 bead per cell ...
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bioRxiv - Immunology 2020Quote: ... The human lymphocytes were isolated from fresh heparinized venous blood via centrifugation over Ficoll gradients (Histopaque-1077; Sigma-Aldrich, Gillingham, United Kingdom) according to the manufacturer’s protocol ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Immunology 2020Quote: ... human TLR4/CD14/MD2 or non-transfected (null-1) were cultured and maintained using DMEM GlutaMAX™ containing 10 % FBS (Sigma-Aldrich), 1 % P/S ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 2 mM L-glutamine and 1% Antibiotic -Antimycotic (Life Tech, UK) with 20 ng/mL human epidermal growth factor (hEGF, Sigma-Aldrich, UK), 20 ng/mL human basic fibroblast growth factor (hbFGF ...
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bioRxiv - Cell Biology 2020Quote: A MilliPlex MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A-Immunology Multiplex Assay (HCYTA-60K-13, Millipore Sigma, Burlington, Massachusetts) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Physiology 2021Quote: Ion channel genes were expressed in human embryonic kidney (HEK) tsA cells obtained from two sources (Prof. R. Horn, Jefferson University, Philadelphia, PA and Sigma-Aldrich #96121229) as described previously (20) ...
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bioRxiv - Neuroscience 2021Quote: ... This iPS cell line was obtained by reprogrammation of a commercial fibroblast cell line (FibroGRO™ Xeno-Free Human Foreskin Fibroblasts, #SCC058, Merck Millipore, Germany). The patient hiPSC cell line was obtained by reprogrammation of skin primary fibroblasts from the female patient II.2 ...
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bioRxiv - Neuroscience 2020Quote: ... medium was changed to Nb/B27 (Mouse) or DDM/B27+Nb/B27 (Human) medium with following chemical compounds: M1 (50µM, Sigma-Aldrich, Cat#SML0629), Mdivi-1 (50µM ...
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bioRxiv - Genetics 2021Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of IMDM (LIFE, 12440061) supplemented with 330 μg ml-holo-human transferrin (Sigma, T0665-1G), 10 μg ml-recombinant human insulin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Either DNA from the BRCA1-methylated HCC38 TNBC cell line or CpGenome™ Human Methylated and Non-Methylated DNA Standards (Sigma-Aldrich) were included in each bisulfite conversion reaction as controls ...
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bioRxiv - Immunology 2020Quote: ... Human breast adenocarcinoma cell line (MCF-7) were obtained (ATCC, Manassas, VA, USA) and maintained in DMEM-F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... This antiserum detects human α-CGRP and β-CGRP but does not cross-react with any other peptide (data supplied by Sigma). We used a rabbit polyclonal antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... or 293CD4 cells (293 cells constitutively expressing human CD4) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS ...