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Citations for Millipore Sigma :
4101 - 4150 of 4506 citations for Recombinant Human CD274 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Single cell solution was obtained from human normal donor and IPF lungs after enzymatic digestion with 10X Liberase (Sigma, 5401127001) and DNaseI (9003-98-9 ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Cancer Biology 2024Quote: ... CWR22 (primary prostate tumour) human prostate cancer cell lines were obtained from ATCC and grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Total protein was extracted from 20-30 mg human frontal cortex using RIPA buffer (PierceTM RIPA Buffer, ThermoScientific, 89901) supplemented with 0.05% CHAPS (Sigma, C3023), 1X complete protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and perfusate levels of 71 analytes using two separate panels as per the manufacturer’s instructions for use: (1) the Human Cytokine 48-Plex Discovery Assay® (HD48; Millipore MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel Immunology Multiplex Assay ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: The cortex tissue samples from mice or human were lysed in PBS along with PDE inhibitor (IBMX) and PIC (SIGMA) with homogenizer followed by centrifugation at 12500 rpm for 20min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... under air-liquid interface conditions in Greens+ keratinocyte proliferation media (Greens media supplemented with 10 ng/mL human EGF (Sigma)) ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown to 70-80% confluency on surfaces were coated with 10μg/ml of purified human fibronectin (SIGMA-Aldrich). Orbital shear stress was applied to the cells for 18-24 h at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: Human fecal samples (10-20 mg) were resuspended in supplemented Brain Heart Infusion (BHI, Sigma-Aldrich, St. Quentin Fallavier, France) as described in(Motta et al. ...
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bioRxiv - Microbiology 2024Quote: ... albumin from chicken egg white (44 kDa) and γ-globulins from human blood (160 kDa) were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2024Quote: BMMCs were sensitized for Ag stimulation with dinitrophenol (DNP) coupled to human serum albumin (HSA) (DNP-HSA, Sigma-Aldrich, #A6661) by pre-loading the FcεRI with 0.15 µg/ml IgE (clone SPE-7 ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: Human HEK293T cell line was grown in Dulbecco’s Modified Eagle’s Medium containing high glucose with HEPES modification (Sigma-Aldrich®, D6171), supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Molecular Biology 2021Quote: Murine or human differentiated brown adipocytes were fixed with 4% PFA and stained with 0.3% Oil Red O solution (Sigma-Aldrich, O0625). The images were observed under a Nikon microscope.
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bioRxiv - Biochemistry 2020Quote: ... The following compounds were selected from the “in-human” collection docking screen and purchased from different vendors: Pterin (Sigma-Aldrich, P1132), Verdiperstat (MedChem Express ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Immunology 2019Quote: Blood derived from healthy human volunteers was collected into citrate tubes (Becton Dickinson) and layered into Ficoll-Paque Plus (Sigma-Aldrich). Samples were centrifuged at 1440 rpm in a swinging bucket centrifuge for 20 min at 23°C without braking ...
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bioRxiv - Biochemistry 2019Quote: ... Cohn fraction IV (Product # G3637) and human whole serum (sterile-filtered from male AB plasma, Product # H4522) were purchased from Sigma-Aldrich, St ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Immunology 2019Quote: ... Cytokine levels in restimulated T cell cultures and SLE patient plasma samples was measured using Milliplex® MAG Human Cytokine/Chemokine Immunoassays (Millipore).
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bioRxiv - Neuroscience 2019Quote: ... transverse paraffin sections of spinal cord mounted on positive charged glass slides were processed for double immunohistochemistry with specific antibodies for human neural stem cells (Sigma-Aldrich) and examined under a fluorescence microscope ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Immunology 2019Quote: ... PerioPaper strips were centrifuged at 3000 × g for 15 min at 4°C in PBS elution for the dosage of the tissue destruction and bone resorption markers by Multiplex (Human MAP, Millipore, USA).
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bioRxiv - Biochemistry 2019Quote: ... and eluted by competitive elution in Flag Wash Buffer supplemented to 200 ug/mL Flag peptide and 250 ug/mL human insulin as a carrier (Sigma-Aldrich). The protein was then passed over a 2 mL amylose column (NEB ...
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bioRxiv - Neuroscience 2019Quote: ... plane through the human temporo-basal neocortex and post-fixed for 30 to 60 min in 0.5 or 1% osmium tetroxide (Sigma, Munich, Germany) diluted in PB-buffered sucrose (300 mOsm ...
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bioRxiv - Systems Biology 2019Quote: Human serum (Cat. No. H6914, from a male AB clotted whole blood, USA origin, sterile-filtered) was obtained from Sigma-Aldrich, St ...
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation, Billerica, MA, USA) was used to measure IgG1 ...
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bioRxiv - Developmental Biology 2020Quote: Female 7-12-week-old FVB/NJ mice were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PSMG) (ProSpec HOR-272) followed by 5 IU human chorionic gonadotropin (hCG) (Sigma CG10) 48 hours later.
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bioRxiv - Neuroscience 2020Quote: Soluble and insoluble human brain samples and mouse brain samples were homogenized in PBS with a protease inhibitor cocktail (catalog no. 11873580001; Sigma Aldrich) with 3.2 mm stainless steel beads ...
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bioRxiv - Cell Biology 2021Quote: ... in the presence of HODHBt or DMSO control and cultured in complete RPMI 1640 medium containing 10% human AB serum (Sigma-Aldrich). As a control ...
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[18F]DCP, First Generation PET Radiotracer for Diagnosis of Radiation Resistant Head and Neck CancerbioRxiv - Cancer Biology 2020Quote: The ex vivo serum stability of the radiotracer [18F]DCP was performed following previously published methods (55–57) and human serum (Sigma Aldrich). [18F]DCP (~1-2 mCi ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (33-35) and selected with 2 µg/mL puromycin (Sigma-Aldrich). To vary glucose concentration ...
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bioRxiv - Biochemistry 2021Quote: ... All wells were then incubated with 3,000-fold diluted goat anti-human IgG-alkaline phosphatase conjugate (Sigma-Aldrich, St. Louis, MO) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: Free floating mouse brain sections were incubated with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO), in activation buffer containing 1.8 mM DTT ...
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bioRxiv - Microbiology 2020Quote: ... One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG, both from Sigma-Aldrich) diluted 1:2,000 in 1% nonfat milk-PBS were then added to each well and the plates incubated for additional 3 h at room temperature before repeating the washing step ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK, 100 IU/ml penicillin and 100 mg/ml streptomycin, Sigma, UK) at a 1:50 ratio for 5-days at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 200 μl of bacterial suspension was added to 400 μl of pre-warmed at 37°C human sera (Sigma-Aldrich S7023), and the mixture was incubated for 2h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... male and primed female frogs were injected with 150 IU and 400 IU of human chorionic gonadotropin (Sigma-Aldrich, Oakville, CA) into the dorsal lymph sac ...
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bioRxiv - Microbiology 2021Quote: Samples were diluted 1:2 in serum matrix for analysis with Milliplex Human Cytokine/Chemokine Magnetic Bead Panel as per manufacturer’s instructions (EMD Millipore Corporation). Concentrations for analytes (EGF ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...