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Citations for Millipore Sigma :
4151 - 4200 of 4506 citations for Recombinant Human CD274 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 cells stably expressing human ACE2 are derived from 293T cells and were maintained in medium supplemented with 2 µg/mL of puromycin (Millipore Sigma) (Prévost et al. ...
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bioRxiv - Immunology 2021Quote: ... IgG-free BSA treatment and blocking prior to opsonization was omitted for beads and surfaces opsonized with human IgG (Sigma, I4506). Free active groups were blocked overnight with 0.5M Tris (pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... gondii tachyzoites were passaged onto Human foreskin fibroblasts (HFFs; ATCC, SCRC-1041) monolayers at 37 °C and 5% CO2 in DMEM (Sigma, D6546) supplemented with 10% FBS (BioSell FBS.US.0500) ...
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Cancer Biology 2021Quote: Human MDA-MB-231 breast adenocarcinoma cells obtained from ATCC (ATCC HTB-26) were grown in L-15 medium (Sigma-Aldrich) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: Cytokine profiling was performed by the Human Immunology Core at the University of Pennsylvania using the Luminex FlexMap 3D system (EMD Millipore). A MILLIPLEX human cytokine 48-plex panel (Millipore Sigma HCYTA-60K-PX48 ...
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bioRxiv - Genomics 2021Quote: Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: GFP+/- LKS and GMPs were sorted as described above and live cells were imaged in 8 chamber borosilicate coverglass system (nunc) coated with human plasma fibronectin (EMD Millipore) using a Leica TCS SP8 confocal microscope equipped with two photomultiplier tubes ...
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bioRxiv - Cell Biology 2021Quote: HUVEC were seeded at 5×104 cells or 2×105 cells in 24-well plates on coverslips coated with 10 μg/ml fibronectin (from human plasma, Sigma Aldrich) and incubated overnight in complete EBM-2 medium ...
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bioRxiv - Cancer Biology 2020Quote: Established human glioblastoma cell line T98G (VTT Technical Research Centre, Turku, Finland in 2010) was cultured in Eagle MEM (Sigma-Aldrich), supplemented with 10% heat-inactivated FBS (Biowest) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30) on a Luminex MAGPIX system (Merck Millipore, Billerica, MA). Conditioned media concentrations (pg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands) and human fibroblasts (gift from Johanna Ivaska, Turku Bioscience, Turku, Finland) were cultured in DMEM (Sigma-Aldrich). T98G (VTT Technical Research Centre ...
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bioRxiv - Neuroscience 2022Quote: Two immortalized cell lines were used to validate the ACE2 receptor, TMPRSS2 and NRP1 assays: Human oligodendroglioma cells (HOG, Cat# SCC163) were obtained from Millipore Sigma-Aldrich (Billerica ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with primary antibodies diluted with TBS + 2% BSA + 0.1% Triton X-100 at 1:1000 (human anti-ACA, Geisel School of Medicine at Dartmouth) and 1:4000 (mouse anti-α-tubulin, Sigma) following the standard immunostaining protocol as described above ...
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bioRxiv - Biochemistry 2022Quote: ... Then cells were stained with anti-mouse IgG Taxes red conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACS Aria II (BD ...
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bioRxiv - Neuroscience 2022Quote: 90,000 human NPCs were plated in a well of Chambered Coverglass wells that had been coated with Poly-L-Ornithine (Sigma Aldrich) and Laminin (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human lung parenchyma and human pulmonary artery were finely minced with scissors in an enzyme cocktail of collagenase I (5mg/ml; Sigma, UK), DNase I (125U/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Multiplex cytokine profiling was carried out on a Luminex MAGPIX system (Luminex) using the Milliplex Human Premixed 41 Plex Immunology Multiplex panel (Millipore Sigma) following the manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in EMEM (Sigma-Aldrich, Cat# M4655-500ML) containing 20% FBS and 1% PS ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of them were analyzed with the MILLIPLEX human cytokine/chemokine magnetic bead panel (HCYTOMAG-60K, Merck Millipore, Burlington, MA, USA) using the Luminex 200 multiplexing instruments (40-012 ...
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bioRxiv - Cancer Biology 2022Quote: ... and NCI-H3122 [H3122] (NCI DTP Developmental Therapeutics Program, male, human) cells were cultured at 37°C in RPMI medium (Sigma-Aldrich) supplemented with 10% FBS (PAN Biotech) ...
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bioRxiv - Cell Biology 2022Quote: ... Serial dilutions of the concentrated supernatants were then incubated with human neutrophil elastase (MilliporeSigma) in the presence of methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide (Millipore Sigma). To quantify bioactivity colorimetric change was quantified using a 96 well plate reader set at 405nm (54).
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bioRxiv - Immunology 2020Quote: Levels of nitrate and nitrite in plasma obtained from human and mice were estimated by a Griess colorimetric assay kit (Sigma chemicals) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Mice were injected subcutaneously under anaesthesia into the right flank with 5 × 106 human bladder cancer cells (RT112) in RPMI medium (Sigma Aldrich) with phenol red-free Matrigel (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: Mating between pairs of adult Xenopus laevis was induced regularly by injections of human chorionic gonadotropin (HCG, 1000 U/ml, Sigma, UK) into the dorsal lymph sacs ...
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bioRxiv - Molecular Biology 2020Quote: ... a single fluorescent cell was sorted per well of a fibronectin-coated (10 μg/mL Human Plasma Fibronectin Purified Protein (Sigma Aldrich) in 1x PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (20) and selected with 2 μg/mL puromycin (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Chips were then washed thoroughly with PBS before incubating with an ECM solution of 150 μg/mL bovine collagen type I (AteloCell, Japan) and 30 μg/mL fibronectin from human plasma (Sigma-Aldrich) in PBS buffered with 15 mM HEPES solution (Gibco ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Bioengineering 2021Quote: ... were coated with 100 μl (5 μg/ml) of purified S1 or S1-RBD or commercial human serum albumin (HSA; Sigma-Aldrich) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... 16 hours post transfection gene expression from the human CMV immediate-early gene enhancer/promoter was induced with 10 mM sodium butyrate (Sigma-Aldrich) for 6 hours before fresh media was added to the cells ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Adipogenic differentiation of ASC was induced during 14 days with human adipocyte differentiation medium [DMEM/F12 50:50 and the following reagents from Sigma-Aldrich Inc. ...
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bioRxiv - Developmental Biology 2022Quote: ... A pair of adults were placed in a 10-gallon tank filled with 10% Holtfreter solution (Holtfreter, 1931) and injected with 10 international units (IU) of human chorionic gonadotropin (HCG; Sigma CG10) into the dorsal lymph sac ...
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bioRxiv - Genomics 2022Quote: ... Mouse gRNA constructs and pCMV-sadCas9-KRAB (kind gift from Dr. Alejandro Lomniczi OHSU) were co-transfected into human preadipocytes X-tremeGene (Sigma, 6366236001) while human gRNA plasmids and pCMV-sadCas9-KRAB were co-transfected into mouse 3T3-L1 preadipocytes ...
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bioRxiv - Molecular Biology 2022Quote: ... Secretion of the following cytokines was quantified with the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex - Immunology Multiplex Assay kit (EMD-Millipore): IL-6 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 mm3 tumor pieces were cut and placed in a 24-well plate containing RPMI medium supplemented with 10% human AB serum (Sigma-Aldrich), 6000 IU/ml human recombinant IL2 (Peprotech) ...
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bioRxiv - Systems Biology 2020Quote: ... one well containing peripheral blood monocyte- or human pluripotent stem cell-derived macrophages were stimulated with 10ng/ml Lipopolysaccharide (LPS) (Sigma-Aldrich; Salmonella enterica serotype minnesota ...
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bioRxiv - Immunology 2019Quote: Multiplex bead assays for plasma samples were performed in duplicate for TNF from the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma). Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Human ovarian cancer cell line OV90 (ATCC CRL-11732) was cultured in 1:1 volume of MCDB 105 medium (Sigma-Aldrich) and Medium 199 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... All other cell lines were grown in Dulbecco`s modified Eagle medium (DMEM) (Fisher, UK) supplemented with 2% human serum (Sigma, UK) and maintained in an humidified sterile incubator with 5% CO2 at 37°C.
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bioRxiv - Molecular Biology 2020Quote: The 3×Flag-CPEB3 plasmid was generated by subcloning the PCR-amplified human CPEB3 cDNA into the p3×FLAG-CMV 10 vector (Sigma-Aldrich). The human CPEB3 cDNA was synthesized using the indicated primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Females were superovulated at 6-8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HNECs were then seeded on 12 mm transwell inserts with 0.4 μm pores (Costar 3460) coated with human placental collagen (Sigma C7521-10MG) at a density of 203,000-333,000 cells per well and maintained in PneumaCult -Ex Plus Medium ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... The serum-free medium –SerM was prepared as described previously by replacing 10% v/v heat-inactivated human serum with oleic and palmitic acid (Sigma Aldrich) in a final concentration of 30 µM each ...
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bioRxiv - Immunology 2021Quote: ... The cells were then treated with SplB (5 μg/mL) or increasing concentrations of human plasma thrombin (PAR1 and PAR4 agonist, Calbiochem-EMD Millipore) or porcine trypsin (PAR2 agonist ...
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bioRxiv - Cell Biology 2021Quote: Human leukaemia cells HL-60 and breast cancer cell line MCF-7 were cultured in RPMI and DMEM media (Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2021Quote: ... falciparum parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...