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Citations for Millipore Sigma :
4151 - 4200 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mice were treated with the non-selective NOS inhibitor N(G)-Nitro-L-arginine methyl ester (LNAME, Sigma). LNAME (10mg/ml made up with sterile saline ...
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bioRxiv - Cancer Biology 2022Quote: Freeze-fracturing of SK-N-SH cells was done as follows: cells were seeded onto collagen-coated (Sigma) sapphire discs in 24-well cell culture plates and differentiated using retinoic acid and BDNF as described above ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils were activated by 30 minutes’ incubation with 100 nM fMLF (N-formylmethionine-leucyl-phenylalanine, Sigma Aldrich #F3506) and 10 μM cytochalasin B (Sigma Aldrich #C2743 ...
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bioRxiv - Cancer Biology 2019Quote: Genotyping to determine p53-/- mice was performed with the REDExtract-N-AMP Tissue PCR Kit (Sigma XNAT-100RXN). Ear punches were digested according to the kit protocol ...
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bioRxiv - Neuroscience 2019Quote: ... 5 or 10 minutes with 100 µM N-Methyl-D-aspartic acid (NMDA, Sigma-Aldrich, St. Louis, MO). Cortical neurons were seeded in T-75 flasks (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: For the measurement of the total protein content 205 μL of Bradford Reagent (Sigma-Aldrich, cat. n. B6916) was pipetted to the 50 μL of BSA standard/thawed cell lysates ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL progesterone (160-24511, Wako, Japan) and 25 μM N-acetyl-L-cysteine (A7250, Sigma-Aldrich)) to remove the mineral oil and M2 medium and seeded on a μ-slide 8 well (80826 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bixafen, (N-(3’,4’-dichloro-5-fluorobiphenyl-2-yl)-3-(difluoromethyl)-1-methylpyrazole-4-carboxamide) (Bixafen, (Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2019Quote: Zebrafish (n = 70) of either sex were anesthetized in 0.03% tricaine methane sulfonate (MS-222, Sigma-Aldrich, E10521). Retrograde labeling of ascending and descending spinal cord neurons located in the spinal segment 15 was performed using dye injections with biotinylated dextran (3000 MW ...
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bioRxiv - Biochemistry 2019Quote: ... The target of interest is biotinylated either through sortagging or labeled with (+)-Biotin N-hydroxysuccinimide ester (Sigma-Aldrich). For NHS labeling ...
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bioRxiv - Microbiology 2019Quote: ... purchased from the listed manufacturers: the complex I inhibitors capsaicin (8-methyl-N-vanillyl-6-nonenamide, Sigma-Aldrich), dihydrocapsaicin (N-(4-Hydroxy-3-methoxybenzyl)-8-methylnonanamide ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Physiology 2020Quote: ... containing 180 µl of 10−4 mol l−1 lucigenin (bis-N-methylacridinium nitrate; Sigma Aldrich, Vienna, Austria) dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight with a primary antibody targeting the extracellular N terminus of GluA1 (MAB2263, Millipore) and then washed with 10% goat serum twice to remove excess primary antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... The two AHL inducers used in this work are N-(3-Oxotetradecanoyl)-L-homoserine lactone (Cin, Sigma O9264) and 3-Hydroxy-C4-HSL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed on ice using HEPES based lysis buffer containing 10 mM NEM (N-Ethylmaleimide, Sigma 04259) and a proteases inhibitor cocktail (1:200; ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual colonies were propagated at 37 °C O/N in 5 mL Luria-Bertani (LB) broth (Sigma-Aldrich) supplemented with 50 mg/mL kanamycin ...
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bioRxiv - Microbiology 2021Quote: ... N-methyl-trimethyl-silyl-trifluoroacetamide (MSTFA) and methoxyamine pyridine hydrochloride were of chromatographic grade and purchased from Sigma–Aldrich ...
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bioRxiv - Genomics 2021Quote: ... and was resuspended in three volumes of lysis solution (0.5 M EDTA at pH 9.0, 1% SDS, 1% N-laurylsarcosine (Sigma), 1 mg/mL of proteinase K (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... were administered haloperidol (0.5 or 2 mg/kg/day; haloperidol; n=12/group: Sigma-Aldrich, Gillingham, Dorset, UK) or vehicle (β-hydroxypropylcyclodextrin ...
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bioRxiv - Microbiology 2022Quote: ... 150 μM (2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF5 compound; Sigma-Aldrich, EF5014), 0.5 μM MitoSOX™ fluorescent probe (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated O/N at 4°C with primary antibodies diluted in 0.1% Triton X-100 (Sigma) blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... D-glucose N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, USA ...
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bioRxiv - Microbiology 2022Quote: ... This pellet was resuspended in ~1.5 ml of 10 mM HEPES + 0.1% (w/v) N-Lauryl sarcosine sodium salt (Sigma) and incubated at room temperature for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The role of MR-PM10 mediated oxidative stress was determined by adding N-acetyl cysteine (NAC; Sigma-Aldrich) to adhesion assays at a final concentration 5 mmol/L ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were spiked with deuterated 17 beta-estradiol and then extracted with n-butyl chloride (Sigma-Aldrich, Inc). The organic layer was dried under nitrogen ...
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bioRxiv - Plant Biology 2024Quote: 0.1% KMnO4 in 0.1 N H2SO4: Add 0.01g KMnO4 (Sigma-Aldrich, Cat. No. 223468, St. Louis, MO, USA) in 10 mL 0.1 N H2SO4 (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... CD1 male (n=10 per group) mice between the ages of 4-6 weeks were given tamoxifen (Sigma) in peanut oil ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 μg of MurA were mixed with 10 mM uridine 5′-diphospho-N-acetylglucosamine (UDP-GlcNAc, Sigma-Aldrich) in a reaction volume of 50 µl containing 100 mM Tris/HCl pH8.0 and 150 mM NaCl as the buffer and preincubated at 37°C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis was then performed by submerging slides in lysis buffer (2% N-Lauroylsarcosine sodium salt (Sigma-Aldrich), 0.5M NA2EDTA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleaved 3HB-SPYcatcher003 with a cysteine residue at the N-terminal was concentrated using Amicon 30K (Millipore), mixed with EZ-link Maleimide-PEG2-Biotin (Thermo A39261) ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified SAH-SPYcatcher003 with a cysteine residue at the N-terminus was concentrated with Amicon 10K (Millipore), mixed with EZ-link Maleimide-PEG2-Biotin (Thermo A39261) ...
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bioRxiv - Neuroscience 2024Quote: ... neurotoxic lesions in ACC were made by infusing N- methyl-D-aspartate (NMDA, Sigma-Aldrich, St. Louis, MO) in a phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse genotypes were determined by PCR using genomic DNA extracted using the REDExtract-N-Amp kit (Sigma XNAT) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we selected N-α-tosyl-ւ-lysine chloromethyl ketone hydrochloride (TLCK, Sigma-Aldrich®, St. Louis, MO, USA) as our trypsin inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... Hemolymph (4 μl/sample) for DAG measurements collected from larvae was diluted in 0.1% N-Phenylthiourea (Sigma-Aldrich) in 50Lμl PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... specimens (n=8 from each group) were sacrificed using a lethal dose (400ppm) of tricaine (MS-222; Sigma) and immediately imaged under light microscopy at 20x before rigor mortis set in to maintain flexibility in the jaw joints ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 10 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion and containing 10% D2O ...
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bioRxiv - Microbiology 2023Quote: ... albicans germ tubes for 20 min and then lysed with n-octyl-β-glupyranoside (#97061-760, Sigma-Aldrich). After preclearing the cell lysates with protein A/G beads (#SC 2003 ...
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bioRxiv - Microbiology 2023Quote: Diflufenican (N-(2,4-difluorophenyl)-2-[3-(trifluoromethyl)phenoxy]-3-pyridinecarbox-amide) was purchased from Sigma-Aldrich (Taufkirchen, Germany). Diflufenican metabolites AE B107137 (2-[3-(Trifluoromethyl)phenoxy]nicotinic acid ...
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bioRxiv - Neuroscience 2023Quote: ... Another cohort of C57Bl6 females were treated with PR agonist segesterone (10 mg/kg, sc, n=10, Sigma) or vehicle (20% β-hydroxycyclodextrin ...
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bioRxiv - Cell Biology 2023Quote: ... N=4 (2 females, 2 males) mice were given 12.5 mg/kg sodium nitrite (52mg/L, 23713, Sigma) in 10% blackcurrant squash (Robinsons) ...
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bioRxiv - Microbiology 2022Quote: ... SK-N-BE(2) cell differentiation was induced by 10 μM retinoic acid (RA) treatment (R2625, Sigma-Aldrich) for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... N-glycosylated proteins were enriched by incubating cell lysates containing protease inhibitor and 1,10-phenanthroline (Sigma-Aldrich, #131377) with 20µl concanavalin A Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... culture media was replaced by culture media supplemented with 1 mg/mL MTT reagent (Sigma, cat n° M5655). MTT reduction to formazan crystals by cellular oxidoreductases were allowed to form by incubating the cells in a 5% CO2 gas-equilibrated humidified incubator at 37°C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Animals were killed by rocking gently (50 rpm) in 7.5% N-Acetyl-L-Cysteine (NAc, Sigma A7250-100G) for 15 min at RT ...
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bioRxiv - Bioengineering 2023Quote: ... N-hydroxy-succinimide sodium salt (Sulfo-NHS; 56485) and L-cysteine (30089) were purchased from Sigma-Aldrich (MO). D-cysteine (A110205-011 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then reduced in 50 mM Tris HCl (pH 8.2) / 2.4 mM sodium deoxycholate / 2.4 mM sodium N-lauryl sarcosine / 5 mM dithiothreitol (all from Sigma Aldrich). Sulfhydryl groups in cysteines were alkylated with 10 mM 2-chloroacetamide (Fluka ...
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bioRxiv - Biochemistry 2023Quote: ... Lysis buffer was used as a positive control of dead cells with tamoxifen (Sigma-Aldrich Cat. N. T5648).