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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Phagosomal proteins from RAW264.7 cells were extracted in 1% sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate (commercially available as RapiGest, Waters) in 50 mM Tris (Sigma) pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The agarose pad was made by mixing 0.5 ml 2% agarose Optiprep mixture (20 mg agarose in 1 ml Optiprep (Sigma), heated to 90 °C ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2022Quote: ... SDS lysate was centrifuged at 20,000 x g for 30 minutes and supernatants were subsequently diluted with 7 volumes of 1.15% Triton-X 100 in TNE with Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) to produce a mixed-micelle lysate with final detergent concentrations of 0.125% SDS and 1% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... DNA plasmids were used at 2 µg/µl and mixed with 1% fast green (Sigma-Aldrich, final concentration 0.2%). Plasmids were injected into the ventricle with a pump-controlled micropipette ...
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bioRxiv - Plant Biology 2022Quote: ... were also amplified by PCR using the primers listed in Supplemental Table 2 and cloned into pGEX4T-1 (Novagen) using BamHI/EcoRI sites to N-terminal glutathione S-transferase (GST ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was re-extracted with 450 µL methanol:water (2:1, v/v) containing 1nmol of scyllo-inositol (Sigma) as an internal standard ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipids were resuspended in chloroform: methanol (2:1 v/v) and loaded onto a silica TLC plate (Sigma 60778). For nonpolar lipid separation ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted in 1% normal donkey serum in PBS with 2 μg/ml DAPI (Sigma-Aldrich, St. Louis, MO, USA) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: Lhx6-Cre;Ppargc1a+/+ and Lhx6-Cre;Ppargc1aF/F animals were injected at P1 with 600nL of custom-made AAV8-hSyn-dio-mito-EmGFP (diluted 1:2 with sterile PBS and 0.5% Fast Green, Sigma). The Vanderhaeghen lab gifted the mito-EmGFP construct ...
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bioRxiv - Neuroscience 2024Quote: ... mixed with AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green (Sigma)) ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:40 dilution for sparse cell targeting and AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green, Sigma). The dilution factor of the DREADDs viruses (here 1:40 ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... 50mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Synthetic Biology 2023Quote: ... A total of 1,000 µL transduction mix containing 1 µL of 2 µg/mL Polybrene (Sigma-Aldrich #TR-1003), recombinant lentivirus ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS plus 0.1% triton (PBST) for 2 hours and then transferred to 1:1000 TH anti-rabbit primary antibody (Millipore) with 1% NDS in PBST overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were finally washed three times and incubated in 4,6-diamidino-2-phenylindole (DAPI) (1 µg/ml; Sigma-Aldrich) for 5 min in dark ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Plant Biology 2023Quote: ... washed three times in sterilised water and sown on half-strength Murashige and Skoog media (1/2 MS) (Sigma), 3 mM MES-KOH (pH 5.7) ...
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Percoll gradients (1-2 per sample depending on harvest density) were formed by vortexing 900 µl Percoll (Sigma P1644) with 100 µl 10x PBS in 2 ml tubes and centrifuging 15 min at 15,000 g ...
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bioRxiv - Neuroscience 2023Quote: ... digestion was performed at an enzyme:protein ratio of 1:100 for 2 hours at 25°C followed by trypsin (Sigma) digestion at an enzyme:protein ratio of 1:50 overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were applied for 2 hours at room temperature and counterstained with DAPI (Sigma Aldrich D9542, 1:10000) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... weighted and incubated in 2 mL lysis solution consisting of 1% v/v Triton-X-100 (108603, Sigma-Aldrich) + 5 mM MgCl2 (M2393 ...
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bioRxiv - Immunology 2023Quote: ... lavage fluids (1/2 diluted; 100 µl) were incubated with 500 µM Gly-Pro-p-nitroanilide substrate (Sigma-Aldrich) in 200 mM Tris-HCl buffer (pH 8.3 ...
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bioRxiv - Microbiology 2023Quote: ... Intestinal tissues were then finely chopped and digested in complete HBSS-2 (HBSS, 25 mM HEPES, 1 mM sodium pyruvate) containing 0.05 mg/mL Collagenase VIII (Sigma) and 50 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... in 2 mL HB buffer (1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, protease inhibitor (Sigma-Aldrich 04693159001), 0.3% IGEPAL-630 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.4) and adjusted to an OD600 of 1.0 in HEPES buffer containing either 1 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt ...
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bioRxiv - Bioengineering 2022Quote: A mixed solution containing 2 μL/mL of Ethd-1 and 0.5 μL/mL of Calcein AM (Sigma-Aldrich) was prepared ...
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bioRxiv - Developmental Biology 2023Quote: ... The dried residues were reconstituted in 50 µL of 2% MeOX in anhydrous pyridine (110-86-1, Sigma-Aldrich) followed by derivatization at 70°C for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... HUVEC or HUAEC cells were transfected with the above lentiviral supernatants diluted (2:1) with ECMB supplemented media with polybrene (8μg/ml) (Sigma) overnight ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... From this 30ul was used to transduce 0.1 million HUDEP-2 cells taken in 24 well plate with 1% HEPES and 3μg polybrene (Sigma-Aldrich). Spinfection was carried out at 800G for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and resuspended in 1-1.5 ml chilled wash and resuspension buffer containing 2% Bovine Serum Albumin (Sigma) and 0.2 U/μl RNase Inhibitor (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS, 1% Triton X-100, 0.5% sodium deoxycholate, 2 mM EDTA, and protease inhibitor cocktail from Sigma Aldrich). Protein content was determined using BCA assay (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Microbiology 2023Quote: The differentiated THP-1 cell lines were seeded into 24-well plates at a density of 1 ×105 cells per well in RPMI-1640 (supplemented with 10% v/v FBS, 1% Penicillin/Streptomycin and 0.05 mM 2-mercaptoethanol) (Sigma) and incubated for 24 hours in 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... The organic fraction was removed from the mixture and diluted 1:2 in 100% ethanol (Sigma cat: E7023-1L). Scanning absorbance values were then taken between 230nm and 290nm on a Nanodrop spectrophotometer ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden hydrogels were cut into smaller pieces and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended in 150 µl of FACS buffer (PBS supplemented with 0.5 % fetal bovine serum and 2 mM EDTA) containing DAPI (1:50,000, Sigma-Aldrich) as previously described (Fischer et al ...
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bioRxiv - Microbiology 2023Quote: Mtb WT and virRmut strains were grown in 7H9 media supplemented with ADC and Tween 80 to an OD 540 of 0.2 and then diluted to an OD540 of 0.1 with 7H9 containing 0 or 50 µgml-1 lysozyme (Sigma). The cultures were incubated with agitation at 37°C and OD540 was monitored daily.
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bioRxiv - Cell Biology 2023Quote: ... buffered with 0.15 mM HEPES (pH = 7.6) and supplemented with 200 mM of 1-Phenyl-2-thiourea (Sigma-Aldrich) to inhibit the melanogenesis ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden were cut into 6x6 mm squares and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 1% FBS and gluMax along with 5 μM CHIR99021 and 2 μM retinoic acid (R2625, Sigma-Aldrich) for 72 hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... pCAG-PiggyBac transposase or PB-pCAG-H2B-GFP (final concentration: 1-2 μg/μL) plasmid was mixed with 0.05% Fast Green (Sigma) and injected into the lateral ventricle of embryos (0.5 μL per embryo ...
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bioRxiv - Evolutionary Biology 2024Quote: Tissue was minced with a scalpel into ∼1 mm3 cubes in 2 mL of digestive solution containing 0.2 mg/mL Liberase TL (05401020001, Sigma). Tissue suspensions were heated at 37°C for 15 minutes and then passed 10 times through a 16-gauge needle attached to a 3-mL syringe ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 100 µg/L in 1× TE buffer with 2 g/L heparin (Sigma-Aldrich, U.K.). Standard solutions from the corresponding RNA stock were also prepared in a 1× TE buffer with 2 g/L heparin to account for any variation in fluorescence ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mM Tris-acetate pH7.5, 2 mM EGTA-Tris, 1% PVP-40, 2mM DTT, 0.5×protease inhibitor cocktail [Sigma-Aldrich]). The protein concentration was determined using a Bio-Rad Bradford protein assay kit (Bio-Rad) ...