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Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 50 μl/well of 3,3’-5,5’-tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (T040, Sigma-Aldrich Co. LLC., St. Louis, MO) was added to the plate ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Immunology 2020Quote: ... Absolute OVA-specific IgG1 concentration was determined for each sample by ELISA as described above using OVA-14 (Sigma, MO USA) as a standard and equalized across all samples ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2019Quote: ... The resulting hybridomas were screened by ELISA for reactivity with STm whole cells and purified LPS (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were washed 4 times by PBST and then 100 µL of 2000-fold diluted anti-strep-HRP (Millipore Sigma) were added into each well for another 1 hr at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated IFX fragments were immobilised on a streptavidin-coated plate and binding of the six mouse monoclonal antibodies was tested by ELISA and detected with an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma-Aldrich) as previously described43 ...
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bioRxiv - Immunology 2022Quote: ... The HIV status of all participants was determined by fourth generation enzyme linked-immunosorbent assay (ELISA) testing and HIV RNA plasma viral load (Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Immunology 2023Quote: ... We confirmed the specificity of our IgA ELISA and non-cross reactivity toward IgG using purified human IgG and IgA (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype control were diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype were control diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma was collected from blood during the GTT to determine insulin levels by ELISA (EZRMI-13K, EMD Millipore Corporation, Burlington, US). Mice were sacrificed at day 7 after a 3h fast ...
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bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Cell Biology 2020Quote: Organs from 6 weeks old wild-type C57BL/6 mice and FLAG-RIPK2 knock-in mice were lysed in IP buffer (150 mM NaCl, 50 mM Tris pH 7.5, 10% glycerol, 1% Triton X-100, 2 mM EDTA, all from SIGMA) supplemented with protease inhibitors (cOmplete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2020Quote: ... gradients (1 for progeny population or 2 for aged population) were formed by vortexing 1.16 mL Percoll (Sigma P1644) with 42 µL 5 M NaCl and 98 µL water in 2 mL tubes and centrifuging 15 min at 15,000 g ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ml Fluorobrite containing 800 μM biotin and 1000 μg/ml L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902) was added to the dish (final concentration 400 μM biotin and 500 μg/ml respectively ...
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bioRxiv - Microbiology 2021Quote: ... T84 and IPEC-1 cells were incubated in DMEM/F12 + 10% FBS + 2 µg/ml Cytochalasin D (Sigma-Aldrich) at 37 ℃ for 1 h to block actin-dependent invasion ...
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bioRxiv - Developmental Biology 2020Quote: ... transgenic and mutant zebrafish embryos were raised at 28°C in Embryo Water (Volvic© water containing 0.28 mg/mL Methylene Blue [M-4159; Sigma] and 0.03 mg/mL 1- phenyl-2-thiourea [P-7629; Sigma]), and staged according to Westerfield (Westerfield ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Genomics 2022Quote: ... RRM1 (clone ID NM_001033.2-476s1c1) and CYP1B1 (clone ID NM_000104.2-1176s1c1) were obtained in pLKO.1-Puro-CMV-tGFP from Sigma Aldrich.
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 2 mM MgCl2 and protease inhibitor cocktail [Sigma]). 10% of lysate volume was reserved to determine total protein amounts ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested in TENT buffer (50 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Cancer Biology 2019Quote: The AsPC-1 and Capan-2 human PC cell lines were obtained from Sigma-Aldrich (St. Louis, MO, USA) and Thermo Fisher (Waltham ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Genetics 2019Quote: ... The differentiating cells were split 1:2 with Accutase and seeded with ROCK inhibitor onto Poly-L-ornithine (Sigma), fibronectin (2ug/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... They were incubated at 28.5°C in 0.3% Danieau medium supplemented 5h after birth with 0.003% (wt/vol) 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to inhibit pigment formation ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM biotin-tubulin were incubated in BRB80 (80 mM PIPES pH 6.85, 2 mM MgCl2, 1 mM EGTA) with 4 mM GTP (Sigma) for 25 min at 37°C in a total volume of 25 ul ...
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bioRxiv - Cell Biology 2019Quote: Larvae used for imaging were maintained in embryo media containing 0.003% 1-phenyl 2-thiourea (PTU, Sigma-Aldrich P7629) starting at 20 hours post-fertilization ...
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bioRxiv - Neuroscience 2019Quote: ... R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy] acetic acid (DIOA; Sigma-Aldrich) and VU0240551 (VU ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... new medium containing decreasing concentration of L19-hTNF (1:10 dilution steps) with actinomycin D (Sigma; 2 μg/mL) was added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: ... at 37°C 1 hr in Lysis buffer 2 (Lysis buffer 1 with 0.5% Triton X-100) and subsequently with proteinase K (Sigma) at 56°C for 2 hrs in Lysis buffer 3 (Lysis buffer 1 with 2% Triton X-100 and 1% SDS) ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was digested for 1 hour at 37°C in 2 mg/mL of Pronase Protease (Millipore, Temecula, CA) in growth media containing 1mM L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... The pellet was thawed by the addition of 2 mL lysis buffer: 1 mg/mL lysozyme (L6876, Millipore Sigma), 10 U/mL benzonase nuclease (E1014 ...
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bioRxiv - Neuroscience 2021Quote: ... containing either 0.01% methylene blue to suppress fungal growth and/or 0.2 mM PTU (1-phenyl-2-thiourea; Sigma-Aldrich) to prevent pigment development ...
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bioRxiv - Cell Biology 2021Quote: ... 12-24hr embryo lysates were collected by homogenizing dechorionated embryos in a Dounce homogenizer with 100 μl of lysis buffer (60mM Tris pH7.5, 80mM NaCl, 6mM EDTA pH8.0, 2% Triton X-100, 5mM 1-Naphthyl potassium phosphate, 2mM PMSF, 1X Sigma Phosphatase Inhibitor II ...
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bioRxiv - Plant Biology 2021Quote: Clean and dry peptide samples were re-suspended in MeCN/H2O (2 %v/v) with formic acid (0. 1 %v/v) and filtered through 0.22 µm filters (Millipore) before being loaded for LC separation ...
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: Adult female worms were incubated with 5 μM of 5’ cy3-labeled Bma-lad-2 siRNA 1 (Sigma Aldrich) for 24 hrs to evaluate uptake of siRNA into intestinal tract epithelial cells ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading/transmigrating cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Biophysics 2020Quote: ... and adding 2 μl of catalase supernatant to 20 μl of 19.1 U⋅μL−1 of glucose oxidase (Sigma). Immediately prior to addition of trapping buffer to the chamber ...
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024 ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings at the one leaf stage were treated in 1/2 Hogland solution (pH 6.0) containing 1.5% NaCl (Sigma-Aldrich) or 20% polyethylene glycol (PEG ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and 80% Buffer Z (0.1 M Na2HPO4, 10 mM KCl, 1 mM MgSO4, and 3.4 µl/ml 2-mercaptoethanol; all from Sigma-Aldrich). Luminescence and absorbance (410 nm ...