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Citations for Millipore Sigma :
4151 - 4200 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cholesteryl hemisuccinate (CHS, C6013) and 1-stearoyl-2- hydroxy-sn-glycero-3-phosphocholine (Lyso-PC) were purchased from Sigma. Brain phosphatidylinositol-4-phosphate (PI(4)P) ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of medium containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (1 mg/mL) (Sigma-Aldrich) was added to each well ...
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bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-NL63 and SARS-CoV-2 were supplemented with 1 µg/ml TPCK-treated trypsin (Sigma-Aldrich, cat. #T1426) increasing the rate of infection ...
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bioRxiv - Neuroscience 2020Quote: ... Bacterial pellets of 20 OD600 units were lysed in 0.5 ml cold lysis buffer (50 mM Sodium-phosphate pH 8.0, 300 mM NaCl, 5% glycerol, 5 mM 2-Mercaptoethanol, 1 mM PMSF [Sigma]), RNase [0.01 mg/ml] ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 2 days at 4°C in goat anti-calretinin (1:200; Cat#AB1550, EMD Millipore), followed by 2.5 hours incubation (RT ...
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bioRxiv - Neuroscience 2020Quote: ... were each diluted in their liquid state to 133.332 Pa (1 Torr) and 266.645 Pa (2 Torr) in light mineral oil (Sigma Aldrich) which also served as the blank stimulus ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs and tumor tissue were stained with αCD8D-1-PsT and αCD8D-2-FITC in PBS supplemented with 0.5% BSA (Sigma) and EDTA (2 mM ...
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were extracted in SDS extraction buffer (50 mM Tris, pH 7.8, 2 % SDS, 1 x protease inhibitor cocktail; P9599, Sigma), 2 mg/ mL NaF ...
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bioRxiv - Developmental Biology 2022Quote: ... transgenic and mutant zebrafish embryos were raised at 28°C in Embryo Water (Volvic© water containing 0.28 mg/mL Methylene Blue [M-4159; Sigma] and 0.03 mg/mL 1-phenyl-2-thiourea [P-7629; Sigma]), and staged according to Westerfield (Westerfield ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: Decreasing concentrations of recombinant DC-SIGN or rfhSP-D (2, 1, 0.5 or 0 µg 100µl/well) were coated on polystyrene microtiter plates (Sigma-Aldrich) at 4°C overnight using carbonate/bicarbonate (CBC ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were washed and then incubated for 2 hours at room temperature in host-specific HRP-conjugated secondary antibody (1:10,000, Sigma) in TBS-T containing 5% skim milk ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 2 days at 4 °C in 0.1M of HCl digestion solution containing 1 mg/ml pepsin (Sigma). Collagen content was measured using the Sircol collagen assay kit (Biocolor) ...
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bioRxiv - Synthetic Biology 2020Quote: Growth experiments in YNB medium supplemented with yeast extract (1 g/L) and 13C-methanol (2 %; Sigma-Aldrich 277177) were carried out aerobically (sparging air ...
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bioRxiv - Cell Biology 2019Quote: ... and re-suspended in CATCH buffer (PBS and 1.5% bovine serum albumin, 1 mM adenosine, 2 mM theophylline, 0.38% sodium citrate, all from Sigma-Aldrich). Platelet counts were determined using a HemaVet counter (Drew Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... paws were incubated twice for 5min with methanol 50% BABB (1/3 benzylalcohol and 2/3 benzylbenzoate, from Sigma), and then three times in pure BABB for 5min each (or until the sample is cleared) ...
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bioRxiv - Cancer Biology 2020Quote: ... For each tested group 2×104 cells were added to 1 ml of 0.4% low-melt agarose solution (Sigma) in OCMI-L media and transferred to a well with a bed of growth media with 0.5% low-melt agarose ...
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5°C for 24h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation.
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bioRxiv - Cell Biology 2020Quote: ... the spheroplasts were recovered in 10 ml EMMA medium (Edinburgh minimal medium with all amino acids and nucleotides supplements) containing 0.8 M sorbitol and 0.5% (v/v) of 1 M 2-deoxyglucose (Sigma, D6134) for 30 minutes at 36°C prior to microscopy imaging.
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bioRxiv - Synthetic Biology 2021Quote: ... The material was then rehydrated by adding 1 × PBS for 5 min followed by incubation with 2% driselase (Sigma) for 60 min at 37°C in a moisture chamber to remove the cell wall ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mg/mL Dox (PanReac AppliChem #A29510025) was also added to drinking water with 1% sucrose (Sigma-Aldrich #S0389) 78,79 ...
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bioRxiv - Zoology 2020Quote: ... We used standard dilutions of hen egg white lysozyme (0.5, 0.8, 1, 2, 4, 8, 10, 20, and 40 µg/ml; Sigma-Aldrich) to prepare a standard curve in each plate ...
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bioRxiv - Immunology 2020Quote: ... remaining 1% PFA was neutralized with 0.1 M glycine in PBS (1 hour) and muscles were blocked with 2% bovine serum albumin (BSA, Sigma), 1% Triton-X (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... 500-1200 nM primers mix (desalt purification, all provided by Sigma-Aldrich Chimie S.a.r.l France, Tables 1 and 2). qPCR mixtures were dispensed by SmartChip Multisample Nanodispenser (MSND ...
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bioRxiv - Microbiology 2019Quote: ... After incubation at room temperate for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Developmental Biology 2019Quote: ... was mixed with 100 μl IP-buffer (50 mM Tris-HCL pH 7.5, 100 mM NaCl, 2 mM EDTA, 1% NP-40, 1x cOmplete EDTA-free protease inhibitor cocktails from Sigma) and antibody (for details on antibodies used see Table S3 ...
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bioRxiv - Cell Biology 2019Quote: ... and then kept in E3 medium supplemented with 0.3 µg/ml of methylene blue and 0.003% 1-phenyl-2-thiourea (Sigma-Aldrich) to prevent melanin synthesis ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... followed by culture on gelatin for 2 weeks in hepato-medium (DMEM:F-12, supplemented with 10% FBS, 1 mg/ml insulin (Sigma), dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... BMDMs were pre-treated with the following inhibitors or vehicle controls: pertussis toxin (Sigma, 1 μg/mL 2 h), SB290157 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... then placed in a 1.7 mL microcentrifuge tube with 1 mL of BY-2 media containing either 0.05% trypsin (Sigma T2601), 0.8% cellulase (Duchefa Biochemie C8001) ...
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bioRxiv - Cell Biology 2020Quote: ... MitoTEMPO ((2-(2,2,6,6-Tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride; #SML0737) and KCN (potassium cyanide; #60178) from Sigma-Aldrich; chemicals applied in Saccharomyces cerevisiae Meyen ex E.C ...
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bioRxiv - Bioengineering 2020Quote: ... according to the manufacturer’s protocol and the cDNA was diluted 1:2 in molecular biology grade water (Sigma,UK). Gene expression analysis was performed by quantitative real time PCR on a RotorGene 6000 cycler (Corbett Research ...
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bioRxiv - Biophysics 2020Quote: ... 10 μL was removed and pulsed into a tube containing 1 μL of 2 mg/mL thermolysin (Sigma Aldrich) for 1 min before quenching with 3 μL 500 mM EDTA ...
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bioRxiv - Microbiology 2021Quote: ... After incubation at room temperature for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Cell Biology 2021Quote: ... Then the gel was washed twice with PBS and coated with 2 ml of 0.2 mg ml−1 rat tail collagen type I (Millipore) at 4 °C overnight.
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bioRxiv - Immunology 2021Quote: ... 0.05μg/mL IL-2 and activated with cell stimulation cocktail (eBioscience) for 1 hour prior to adding 10μg/ml brefeldin A (Sigma) and culturing for a further 4 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μL droplets containing a total of 1000 cancer and stellate cells in a 1:2 ratio were prepared in a 0.24% solution of methylcellulose (M0512, Sigma) and plated on the underside of a tissue culture plate lid ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Molecular Biology 2022Quote: ... and pellets were resuspended in NP40 buffer (20 mM Tris pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% NP40) and treated with Benzonase (Sigma). Anti-HA magnetic beads (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... and 80% Buffer-Z (0.1 M Na2HPO4, 10 mM KCl, 1 mM MgSO4, and 3.4 µL/mL 2-mercaptoethanol; all from Sigma-Aldrich). Luminescence and absorbance (410 nm ...
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bioRxiv - Plant Biology 2022Quote: ... as well as commercially available mouse anti-alpha-tubulin (Sigma-Aldrich, USA, cat. no. T9026-2, dilution 1:300), rabbit anti-histone H3K4me2 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl plasmid vectors (2 μg) and/or siRNA oligos (0.1 nmol) containing 0.05% fast green FCF (Sigma-Aldrich) were injected into each DRG ...
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bioRxiv - Biochemistry 2022Quote: Phagosomal proteins from RAW264.7 cells were extracted in 1% sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate (commercially available as RapiGest, Waters) in 50 mM Tris (Sigma) pH 8.0 ...