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Citations for Millipore Sigma :
4151 - 4200 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane that was blocked with 5% skim milk (Sigma) in PBS overnight and incubated with rabbit antiserum against ACS (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... The protein solution was then concentrated using an Amicon Ultra 15 Ultracel-10K device (Millipore). Samples were desalted using a Millipore Ziptip C18 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated to 12 mg/mL using an Amicon Ultra Centrifugal Filter (Merck-Millipore). To purify non-degraded PrgA (without the S476A mutation) ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to a 0.45 μm pore size polyvinylidene fluoride transfer membrane (Merck Millipore), and nonspecific binding was blocked in Tris-buffered saline/0.1% Tween 20 with 5% skim milk powder ...
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bioRxiv - Bioengineering 2021Quote: The proteins were concentrated using Amicon® Ultra Centrifugal filters (Millipore, 100-kDa cut-off) and purified using size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2020Quote: Proteins were cross-linked to DNA by the addition of 37% formaldehyde (Sigma-Aldrich; F8775) to a final concentration of 1% for 10 min at the desired time of analysis ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant containing solubilized SLC44A1 protein was load to anti-FLAG M2 affinity gel (Sigma), then washed by Lysis Buffer with 0.02%DDM and 0.004% CHS ...
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bioRxiv - Neuroscience 2021Quote: Luminex multiplexed immunoassays were used to quantify phospho-proteins within the MAPK (Millipore 48-660MAG) and PI3K/Akt/mTOR (Millipore 48-612MAG ...
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bioRxiv - Neuroscience 2021Quote: ... pathways as well as 32 panel of cytokine/chemokine protein expression (Millipore MCYTMAG-70K-PX32) in brain lysates from the Rosa26TurboID/wt AAV and transgenic cohorts ...
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bioRxiv - Neuroscience 2021Quote: ... a quantity of 3.5 μg of protein digest was purified on a Ziptip C18 (Millipore), dried and re-suspended in 100 mM Ammonium formiate (pH 10 ...
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bioRxiv - Neuroscience 2021Quote: ... resolved protein bands were transferred onto Polyvinylidene Fluoride membranes (PVDF, 0.45μm, EMD Millipore, ON, Canada) for 75 minutes on ice in a transfer buffer containing 25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: Premature chromosome condensation was induced using the protein phosphatase 1 inhibitor calyculin (Sigma, C5552-10UG). To determine the cell cycle stage of prematurely compacted chromosomes ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted with RIPA buffer supplemented with protease inhibitor cocktails I and II (Sigma) and phosphatase inhibitor cocktail set III (Calbiochem) ...
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bioRxiv - Cancer Biology 2020Quote: ... total proteins were extracted from cells using ice-cold CHAPS lysis buffer (Sigma-Aldrich, #S7705) and real-time Q-TRAP performed with 0.1 μg protein extracts ...
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bioRxiv - Cell Biology 2021Quote: Proteins pellets from lipid droplet-enriched fractions were resuspended in 0.1 M NaOH (Sigma-Aldrich) and subsequently neutralized using 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein of interest was purified by over-night incubation with anti-FLAG (Sigma, F1804) or anti-RFA antibody (Agrisera ...
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bioRxiv - Microbiology 2020Quote: ... Immunoreactive protein bands were visualized by the NBT-BCIP method (Sigma-Aldrich™ Argentina S.A). Intensities of bands were quantified from scanned images using ImageJ software and the value obtained from each band was normalized by the loading control SAG1 and then each histone PTM was normalized by their respective histone (H3 or H4).
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bioRxiv - Microbiology 2020Quote: ... the sequence encoding mature proteins without the signal peptide was cloned into pET-14b (+) (Novagen) to generate the expression vector pET14b-6xHis-BcCrh1 ...
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bioRxiv - Systems Biology 2021Quote: ... proteins were transferred onto a PVDF membrane (pore size 0.45 mm, Immobilon-P, Millipore, www.millipore.com) (66 ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was extracted using an extraction buffer comprised of 50 mM MOPS (M1254, Sigma) pH 7.0 ...
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bioRxiv - Neuroscience 2021Quote: ... the protein bands were visualized through the addition of super enhanced chemiluminescent (ECL) (Millipore, USA) for exposure and imaged by a Gel Image System (Bio-Rad ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: Protein samples were separated on SDS–polyacrylamide mini–gels and blotted onto PVDF membranes (Millipore). As primary antibodies ...
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bioRxiv - Microbiology 2021Quote: Protein samples were resuspended in 200 µL of 100 mM triethylammonium bicarbonate (TEAB) (Sigma-Aldrich), reduced with 20 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2021Quote: ... 60 pmol of sgRNA was complexed with 10 pmol of Cas9 protein (Sigma-Aldrich, #CAS9PROT) and electroporated into 0.5*105 Jurkat cells per reaction ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine protein contents in lung homogenates were quantified using Luminex multianalyte technology (Millipore) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of the gel were then stained with Coomassie blue R-250 (Sigma-Aldrich, USA) and the molecular mass of the proteins was determined with reference to standard proteins (Thermo Scientific ...
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bioRxiv - Physiology 2021Quote: ... the proteins were transferred to Millipore Immobilon®-P Transfer Membrane (Millipore, Billerica, MA, USA) and blocked in 5% skin milk solution for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins were eluted two times with 600 μL 250ug/mL 3xFLAG peptides (SIGMA, F4799) in TBS [50mM Tris-HCl pH 7.4 ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were concentrated to ~10-15 mg/ml using 100 kD cutoff centrifugal devices (Millipore), flash-frozen and stored at −80 °C.
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bioRxiv - Systems Biology 2021Quote: ... precipitated SIL-proteins were reconstituted in 104 μL of 8 M urea ([UA]; Sigma-Aldrich) in 0.1 M Trizma® base (pH 8.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted proteins were concentrated using 10-kDa molecular weight cut off filters (Amicon Ultra, Millipore) and washed with ddH2O ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were purified from the lysate with anti-FLAG M2 affinity gel (Millipore-Sigma, USA) and eluted with 0.2 mg/ml 3xFLAG peptide in elution buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were purified from the lysate with anti-FLAG M2 affinity gel (Millipore-Sigma, USA) and eluted with 0.2 mg/ml 3xFLAG peptide in elution buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: The DRM and DSM fractions119 were separated using the CelLytic MEM Protein Extraction Kit (Sigma). 600 μl of lysis and separation buffer were added to 50 μl of resuspended membrane and incubated at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibody against HCoV-OC43 nucleocapsid (NP) protein was purchased from Millipore (Cat.No. MAB9012). Rabbit polyclonal antibody against human LY6E was obtained from proteintech (Cat.No ...
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bioRxiv - Microbiology 2019Quote: ... PVDF membrane-bound proteins were first probed with mouse anti-HA (1:3000; Sigma, H3663) and rabbit anti-GAPDH (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-P or Immobilon-FL, Millipore, Burlington, MA) and blocked for 1 h at room temperature in 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto methanol-preactivated Immobilon-P PVDF membranes (pore size 0.45 μm; Millipore) using a semi-dry transfer cell or a wet transfer tank (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... The sypro orange protein gel stain was purchased from Sigma Aldrich (Merck KGaA, Darmstadt, Germany). The integrated potato culture medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the polyacrylamide gel to a PVDF membrane (Merck Millipore, Massachusetts, USA). Membranes were blocked with Rockland blocking buffer (Biomol GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing grade-modified trypsin (Sigma). Protease digestion was stopped by addition of trifluoroacetic acid to 0.5% and precipitates were removed by centrifugation ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... RIPA buffer was not used and the protein concentration was measured with Bradford assay (Millipore).
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bioRxiv - Biophysics 2020Quote: Hen egg white Lyz and bovine β-Lac proteins were both purchased from Sigma-Aldrich and used with no further purification ...
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bioRxiv - Biophysics 2020Quote: ... Protein was concentrated and buffer exchanged on Amicon Ultracel-10K centrifugal filters (Millipore; Darmstadt, Germany), dialyzed exhaustively into 10 mM MOPS ...
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bioRxiv - Cancer Biology 2021Quote: ... 80 μg protein extract from each sample was reduced with 50 mM dithiothreitol (Sigma, #43815) at 95 °C for 10 min and then alkylated with 100 mM iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amount of protein was loaded to the anti-Flag M2 Magnetic beads (M8823, Sigma) and anti-HA Magnetic beads (88836 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by SDS-PAGE (8% acrylamide gel) and transferred to nitrocellulose membranes (Millipore). Membranes were incubated overnight at 4°C with a primary antibody diluted in Tris-buffered saline plus 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2021Quote: ... The dialysed protein was concentrated in a 10 kDa cut off Amicon Ultra-15 (Millipore), quantitated using BCA ...