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Citations for Millipore Sigma :
4001 - 4050 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding 0.5 mM isopropyl β-Dthiogalactopyranoside (IPTG; Sigma Aldrich). After 20h incubation at 15°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
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bioRxiv - Biochemistry 2023Quote: ... the protein was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 41.46 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Developmental Biology 2023Quote: ... Then proteins were transferred onto a 0.45-μm polyvinylidene fluoride membrane (Sigma Aldrich, IEVH00005) and the membranes were blocked in TBST containing 5% skimmed milk for 1 h at room temperature and then incubated with the indicated primary antibodies overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... GFP-V5 or GFP-FLAG fusion proteins using OptiMem and Fugene HD (Sigma-Aldrich). Forty-eight hours later ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Cancer Biology 2023Quote: ... Separated proteins were then transferred to Immobilon®-P PVDF Membranes (Milipore Sigma #ISEQ00010) using the BioRad Transblot SD Semi-dry Transfer Cell as per manufacturer’s recommendations.
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma) in 25 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred to an Immobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore), and the membranes were incubated with Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were similarly incubated with HA binding protein (HABP) (EMD Millipore 385911, Burlington, MA), then detected with an appropriate fluorescent secondary antibody for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Pathology 2023Quote: Protein was extracted by 1x RIPA lysis buffer (10x, stock solution, EMD Millipore, MA) from mouse stomach tissue ...
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bioRxiv - Plant Biology 2023Quote: Proteins were transferred to a PVDF membrane (Immobilon-P, Millipore Corporation, Billerica, MA, USA) via semi-dry Western blotting ...
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Dynamics of the epigenetic landscape during development and in response to drought stress in sorghumbioRxiv - Plant Biology 2023Quote: ... H2A.Z (preparation using antigen “GKGLLAAKTTAAKSAEKDKGKKAPV”) were incubated with Protein A agarose (16-157, Millipore) before mixed with sonicated chromatin ...
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bioRxiv - Biophysics 2024Quote: ... The dialyzed protein solution was filtered through a 0.22 μm membrane (Merck Millipore, SLGP033RB) and injected onto an ion-exchange Q column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... The expression of proteins was induced with tetracyclin (1 µg/mL; Sigma-Aldrich, T7660). Cell were treated with actinomycin D (10 ng/mL ...
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bioRxiv - Cell Biology 2024Quote: PLA in cultured PAEC were performed using Duolink PLA protein detection technology (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were stained for total protein using Ponceau S (Sigma-Aldrich; St. Louis, MO) and subsequently probed with Aldehyde dehydrogenase 1-A1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Molecular Biology 2023Quote: ... separated by SDS-PAGE and proteins transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked in 5% milk in PBS/ 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: RIP was performed using a Magnetic RIP RNA-Binding Protein Immunoprecipitation (Millipore, 17-700) by the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... and protein complexes were eluted with 150 mg/ml of 3X FLAG peptide (Sigma). Samples were diluted in 2X SDS sample buffer and analyzed with SDS–PAGE according to standard procedures ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins were incubated overnight with the anti-FLAG M2 (Sigma M8823) or anti-HA (Pierce #88836 ...
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bioRxiv - Microbiology 2023Quote: ... followed by protein precipitation after the addition of trichloroacetic acid (TCA, Sigma-Aldrich, 91228), (v:v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved in 10% SDS/PAGE and transferred to nitrocellulose membranes (Sigma Aldrich). Membranes were probed for 2 h at RT (or overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Elution step of enriched proteins was performed using 2.25 M ammonia solution (Sigma, #5.33003) for 16 h ...
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bioRxiv - Biochemistry 2024Quote: ... The antibody-lysate complex was incubated with protein A- conjugated sepharose beads (Sigma #P3391) and eluted in 2X laemelli dye ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were precipitated by adding 100 μl of gamma-globulins (10 mg/ml; Sigma) and 2 ml of 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Developmental Biology 2023Quote: ... supernatant containing protein suspension was collected and quantified with Bradford reagent (Sigma, B6916-500ML) at the spectrophotometer ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in TBS buffer supplemented with 5mM d-desthiobiotin (Sigma Aldrich). Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Neuroscience 2023Quote: ... PLP (Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 MAB388 Sigma-Aldrich, 1:200), MHCII (Dako 1:50) ...
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, USA) and probed with primary antibodies overnight ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: Incubation mixtures (30 µl) contained 10 µM of protein and 5× SYPRO Orange (Sigma) in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted protein was further concentrated using a spin concentrator (10 kDa Amicon, Millipore) and rebuffered in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: Protein content in mitochondria-enriched fractions were estimated using Bradford Assay reagent (Sigma-Aldrich) followed by solubilisation as previously reported by Rawat et al21 ...
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bioRxiv - Genetics 2024Quote: ... proteins on the SDS-PAGE were transferred to a PVDF membrane (Millipore, Billerica, MA) at 100V for 3 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Pathology 2024Quote: Protein samples were extracted using radioimmunoprecipitation buffer containing inhibitors of proteases (cOmplete, Sigma-Aldrich) and phosphatases (PhosSTOP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... protein levels was done using heparin affinity columns (Sigma-Aldrich, St. Louis, MO, USA) and Western blot analysis as previously described [37 ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Bioengineering 2024Quote: ... After dialysis the protein was concentrated through Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cutoff of 3 kDa ...