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Citations for Millipore Sigma :
4301 - 4350 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 100K (Merck Millipore) and stored at -80°C ...
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bioRxiv - Immunology 2020Quote: ... and 10 mg of protein per experiment were reduced with 10mM dithiothreitol (DTT, Sigma, #D0632) for 1 hour at 55°C and alkylated with 20mM iodoacetamide (IAA ...
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bioRxiv - Genetics 2019Quote: Proteins were precipitated in 23% TCA (Sigma-Aldrich, St. Louis, MO, Product number T-0699) at 4 °C O/N ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing-grade modified trypsin (Sigma). To purify the peptides ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and the purified proteins were concentrated with Amicon Ultra-0.5 Centrifugal Filter devices (Merck Millipore) following manufactures instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The eluted proteins were concentrated 5X with 10KDa-cutoff Amicon centrifugal filter unit (Millipore, #UFC501024), and analyzed by native gels and Western blotting.
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bioRxiv - Physiology 2019Quote: ... Data were normalized by protein content of the homogenate measured by Bradford assay (Sigma Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... followed by an overnight incubation with Pure Proteome Protein A/G mix magnetic beads (Millipore). Samples were washed as indicated by the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated using a 30 kDa MWCO centrifugal filters (Amicon Ultra; Merck Millipore) and then gel filtered ...
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was induced by 0.2 mM Isopropyl-²-D-thiogalactopyranoside (IPTG; Sigma-Aldrich, Taufkirchen, Germany) and continued at 16 - 20°C for 18 - 20 h ...
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bioRxiv - Biochemistry 2020Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Immunology 2021Quote: S protein at 1mg/ml in PBS was cross-linked with 4% formaldehyde (FA) (Sigma) overnight at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The remainder of the samples was incubated for 1hr with uncoated protein G beads (Millipore) to remove a-specifically bound proteins ...
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bioRxiv - Cell Biology 2021Quote: ... The protein sample was concentrated in an Amicon Ultra 15 centrifugal filter (Millipore Sigma, UFC900308) to a concentration 1mg/ml and injected into Guinea Pigs (Pocono Rabbit Farms and Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000 ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... The protein was eluted with buffer C (buffer B+10%glycerol+2.5 mM Desthiobiotin (Novagen)) in four column volumes ...
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bioRxiv - Biochemistry 2021Quote: ... Expression of Clover-MgtA fusion proteins was induced by addition of 0.2 % L-arabinose (Sigma) in exponential growth phase for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... Purified protein was concentrated with Amicon Ultra-0.5 mL centrifugal filters (50kDa cutoff; EMD Millipore) to 100 µL ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mg protein were incubated with ANTI-FLAG® M2 Affinity Gel (Sigma, cat.no. A2220) for 2 h on a rotator at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: Total protein was extracted from thirty zebrafish embryos using cell lysis buffer (Sigma-Aldrich, C3228). Purified protein was then digested into peptides using Trypsin (Promega ...
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bioRxiv - Biophysics 2021Quote: Proteins and peptides were labelled at their N-terminus with Fluorescein 5-Isothiocyanate (FITC, Sigma), purified and quantified following a described protocol [45] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein extracts were prepared and subjected to immunoprecipitation with anti-FLAG M2 magnetic beads (Sigma) as previously described [25] ...
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bioRxiv - Physiology 2021Quote: ... or amyloid precursor protein (0.6 mg/kg, sAPPα, S9564 Sigma Aldrich Solutions, St. Louis, MO) injection ...
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bioRxiv - Cell Biology 2021Quote: ... samples were treated with the protein synthesis inhibitor 10 μM cycloheximide (Millipore Sigma C4859-1ML) for 10 minutes prior to puromycin treatment and during puromycin treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested overnight with trypsin from bovine pancreas (Sigma-Aldrich, St Louis, MO, USA) at 37 °C at a 50:1 (m/m ...
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bioRxiv - Cancer Biology 2020Quote: ... wild type and mutant proteins were treated similarly with calpain 1 (Sigma-Aldrich, Cat. #C6108) in vitro and their cleavage was assessed by immunoblotting.
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bioRxiv - Cancer Biology 2020Quote: ... The FLAG M2 antibody used for the detection of MetRSL274G protein was purchase from Sigma. ANL ...
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bioRxiv - Cell Biology 2020Quote: ... The bound protein was eluted four times with 250 μL of HA peptide (Sigma-Aldrich) (0,1 mg/ml).
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Microbiology 2020Quote: ... Outer membrane proteins of these bacteria were subsequently labelled with fluorescein isothiocyanate (FITC, Sigma-Aldrich) as previously described [57] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 10% SDS-PAGE gel followed by electrotransfer to PVDF membranes (Millipore). The membranes were blocked with TBST supplemented with 10% skim milk for 30 mins at room temperature and further probed with primary antibodies at 4°C overnight including Trim17 (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using primary antibodies α-FLAG (monoclonal α-FLAG M2, Sigma, 1:1000) or α-Myc (Clone A-14 ...
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bioRxiv - Microbiology 2021Quote: ... The protein concentration was measured by Coomassie Brilliant Blue assay using bovine serum albumin (Sigma) as standard ...
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bioRxiv - Neuroscience 2021Quote: ... the isolated cell populations were immediately placed in 20μl RIPA protein extraction buffer (EMD Millipore) with HALT Protease/Phosphatase inhibitors (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Cellular proteins were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore IPVH00010) using standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were purified from hybridoma culture supernatant using protein G Sepharose 4 Fast Flow (Sigma). Injection of the respective antibody resulted in depletion of >90% of CD4+ or CD8+ T cells for at least 14 days ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound proteins were neutrally eluted with 50 µL of V5 peptide (V7754-4MG, SIGMA) at 1mg/mL in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 stocks were additionally cleared using a 0.45 μM low protein binding filter (Millipore) to remove mucus debris produced by these cells and the medium was exchanged three times for Opti-MEM I (1X ...
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bioRxiv - Immunology 2022Quote: ... The alkylation of the proteins was carried out by adding chloroacetamide (Sigma-Aldrich, Saint-Louis) to a final concentration of 7.5 mM followed by a 20 minute incubation in the dark at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Proteins in supernatants were then precipitated by incubating with 0.61 N trichloroacetic acid (Sigma-Aldrich) plus 1× protease inhibitory cocktail (PIC ...
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bioRxiv - Cell Biology 2022Quote: ... followed by the addition of protein A/G beads (10 ul suspension; PureProteome, EMD Millipore) for an additional 5 h on an orbital plate ...
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bioRxiv - Immunology 2022Quote: ... Bound proteins were eluted by incubating the beads with 200µg/ml 3xFLAG peptide (Sigma- Aldrich) in IPP150 containing 0.02% NP-40 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-PSQ membrane (0.2 µm; Millipore). Immunoblotting was performed using the following primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA binding proteins including target RNAPs were precipitated by addition of polyethyleneimine (PEI, Sigma-Aldrich) to 0.6% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was degraded by adding 2 µL 18.2 mg/mL Proteinase K (Millipore Sigma 3115887001) and incubating at 55°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were rinsed in milliQ water and protein transfer was checked by Ponceau staining (Sigma). Transferred membranes were washed once with TBS and three times with TBS-Tween (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were transferred onto a buffered poly-vinilidine di-fluoride (PVDF) membrane (Millipore, Germany). The non-specific staining was blocked by 5% skimmed milk protein (1X TBST;4hr ...