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Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were washed 2 x 1 hour in 0.2% Tween-20 (Sigma) in 1X PBS with 10 µg/ml heparin (Fisher ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay (MTT assay, Cat. No. M5655, Sigma-Aldrich, St. Louis, MO). Cells were plated in 96-well plates ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Head extractions were incubated at increasing concentrations of the water-soluble standard cardenolide ouabain (10−8 M to 2 × 10−3 M, Sigma, Germany, O3125-1G) at 37°C under the following conditions ...
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bioRxiv - Genetics 2021Quote: ... Zebrafish embryos and larvae were anesthetized in 160ug/ml Ethyl 3-aminobenzoate methanesulfonate (Tricaine, MS-222) C9H11NO2·CH4SO3 (Sigma-Aldrich, 886-86-2) in E3 embryo media (Westerfield ...
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bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical], 1.2 units/mL Dispase II [Sigma-Aldrich], in perfusion buffer) for ∼45 minutes at 37° C using a rotating holder ...
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bioRxiv - Cancer Biology 2021Quote: Cytotoxicity of USP7 inhibitor P5091 and P22077 was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay (Sigma-Aldrich, St. Louis, MO, USA). Cytotoxicity was assessed after 24 hrs and 48 hrs of inhibitor treatment in 96 well plates.
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich, Dorset, UK). All probes were supplied by Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, purity > 98%) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: The following chemicals in high grade were obtained commercially or as a gift: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) (Sigma-Aldrich, St Louis, MO, USA); Raloxifene (Cipla Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... a RRP17-HA-AID strain was transformed with plasmids carrying respective constructs and growth assessed upon Rrp17-HA-AID depletion on plates containing 2 mM auxin (3-indoleacetic acid) (Sigma-Aldrich, Cat# I2886). Genetic experiments were performed at least in two biological replicates.
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bioRxiv - Neuroscience 2024Quote: ... at least 3 times and incubated at room temperature for 2 h in blocking solution containing 5% normal horse serum (Sigma-Aldrich, Cat. # H0146) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 200μl media including 10 μL of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide, 5mg/mL) (Sigma-Aldrich, U.S., Cat. No. M2272) per well for 4h at 37°C (Ayazoglu Demir et al. ...
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bioRxiv - Neuroscience 2024Quote: ... aCSF and 192IgG-SAP groups received intraperitoneal (ip) injections of WIN55,212-2 (0.5 mg/kg or 3 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) or vehicle solution (1:1:18 ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were permeabilized with 0.2% Triton X-100 (Nacalai Tesque, Kyoto, Japan) for 15 minutes and blocked with 3% bovine serum albumin (BSA: Sigma Aldrich, MO, USA) for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 months old mice were orally gavaged with 3 x 2 (Mist1creERT/+KRASG12D/+) or 5 x 5 mg (Ptf1acreERT/+KRASG12D/+) of tamoxifen (TX; Sigma-Aldrich, T5648-5G) at Western University and 5 x 4 mg (Ela-creERT ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Neuroscience 2021Quote: ... antisense: 5’-ACCGAUUGAAGUUAAUGUC-3’), Myef2 (sense: 5’-CUUUGGUGGAGUUGGCCGA-3’, antisense: 5’-UCGGCCAACUCCACCAAAG-3’) or siRNA universal negative control (SIC001, Sigma-Aldrich) were purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Then the supernatant was separated by size to a large (>3 kDa) and small (<3 kDa) fraction using Amicon ultrafiltration system with 3 kDa filter (Millipore, Ireland). The fraction then filtered again through a 0.22 µm (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... N-(3-Oxoocatnoyl)-L-HSL (>97%) (3-Oxo-C8) and N-(3-Oxododecanoyl)-L-HSL (>98%) (3-oxo-C12) were used as received from Sigma-Aldrich. N-(3-Hydroxy-7-cis-tetradecanoyl)-L-HSL (>95% ...
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bioRxiv - Plant Biology 2021Quote: ... Five days after germination the plant were incubated in liquid in ½ MS with 1% sucrose media with or without 50 µM 2-bromopalmitate (2-BrP; Sigma-Aldrich), and with or without 100 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Strains were streaked from frozen stock onto yeast extract peptone dextrose (YPD; 1% yeast extract, 2% peptone, 2% dextrose, Sigma-Aldrich) agar and left overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Plant Biology 2022Quote: ... 0.85 % saline (2 mins), Phosphate Buffered Saline (PBS) (2 mins), 0.125 mg ml-1 Pronase (37°C, 10 mins) (Sigma-Aldrich, P2308), 0.2 % Glycine (2 mins ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with PBST and then incubated for 2 h at room temperature in PBS with 2 μg mL−1 DAPI (D9542, Sigma-Aldrich). Cells were imaged using an LSM980 confocal microscope (Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
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bioRxiv - Biophysics 2023Quote: Crude and low-dense MVs were exposed to SCL medium supplemented with CaCl2 (2 mM) and either 2 mM Na2HPO4 or 1 mM adenosine triphosphate (Sigma-Aldrich). Mineralization was carried out at 37°C and using samples with fixed 50 µg of total protein/mL concentration ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Developmental Biology 2021Quote: ... for phosphorylated histone 3 antibody staining (Chen et al., 2020) (pH3, Sigma # 05-806, 1:100), or with Lavdovsky’s fixative (3.7% formaldehyde ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were washed 3 times and incubated for 30 minutes with streptavidin Cy3 (Sigma, 1:500). All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were diluted 1 uL into 3 uL water before monitoring peptide formation electrophoretic TLC (Millipore). TLC plates were equilibrated with pyridine acetate buffer (5 mL pyridine ...
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bioRxiv - Bioengineering 2019Quote: ... A control sample of 1 mg/mL PbS quantum dots (Millipore Sigma, 3 nm nominal size) in toluene was run first to verify measurement accuracy ...
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bioRxiv - Biophysics 2020Quote: ... 100 µl of a 1:1000 dilution of 3 µm polystyrene beads (LB30, Sigma Aldrich, Germany) were added and after a 3-minute incubation ...
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bioRxiv - Genetics 2021Quote: ... late L4 animals were mock treated or exposed to 1 mM auxin (3-indoleacetic acid, Sigma) for 24 h ...
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bioRxiv - Bioengineering 2021Quote: Cell pellets and EVs solutions (in 3:1 ratio) were lysed in RIPA buffer (Sigma-Aldrich) containing the Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times for 15 min using 1× PTW and mounted in 87% glycerol (Sigma-Aldrich)/ddH2O containing 25 mg/ml DABCO (Roth/Lactan) ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Antibodies were diluted in 3% normal donkey serum (monoclonal mouse anti-mouse GFAP 1:2000, Sigma G-A-5 ...
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bioRxiv - Neuroscience 2020Quote: ... Then washed for 5 min x 3 times and blocked in 1% BSA (A3059, Sigma-Aldrich) (w/v ...
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bioRxiv - Genetics 2022Quote: Indole-3-acetic acid powder (125 mg) was dissolved in 1 mL PBS (Sigma-Aldrich, D8537), with small quantities of NaOH (IGC Technical Services ...
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bioRxiv - Cell Biology 2022Quote: ... was diluted 1:100 (v/v) in 3% (w/v) bovine serum albumin (BSA, Sigma-Aldrich) in TBSTEM buffer (10 mM EGTA ...
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bioRxiv - Bioengineering 2022Quote: ... Germany) and covered with a solution of 1 % (3-Aminopropyl)triethoxysilan (APTES, A3648, Sigma-Aldrich, USA) dissolved in DI water ...
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bioRxiv - Neuroscience 2021Quote: ... The brain sections were then homogenized in a 3:1 PBS: trichloroacetic acid (TCA) (Sigma-Aldrich) solution to precipitate out macromolecular compounds ...
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bioRxiv - Developmental Biology 2021Quote: ... in 1XPBS] and immunostained using primary antibodies: mouse anti-Phospho histone-3 (1:200) (Sigma-Aldrich); Rabbit anti-Drosophila caspase-1 (DCP-1 ...