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Citations for Millipore Sigma :
3901 - 3950 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Microbiology 2024Quote: ... parasite pellets were placed on ice for 5 min before addition of 50 μl chloroform followed by 200 μl methanol:ultrapure water (3:1, including scyllo inositol as an internal standard, 1 nmol, Sigma-Aldrich, I8132). Extraction was facilitated through vigorous vortexing ...
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bioRxiv - Microbiology 2024Quote: For the leukopenic model mice were immunosuppressed with 150 mg/kg of cyclophosphamide (Baxter) on days −3 and −1 plus 1 subcutaneous injection of 250 mg/kg hydrocortisone 21-Acetate (Sigma-Aldrich) on day −1 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with fresh protease inhibitors (3 µg/mL Leupeptin, 1 µg/mL macroglobulin, 1 µM benzamidine, 0.5 µM PMSF) and phosphatase Inhibitors (EMD Millipore, 524625). Cell lysates were incubated 10 minutes on ice and centrifuged at 13000 rpm for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 1% BSA, 1 mM DTT, 0.1 U/µl Sigma Protector RNase Inhibitor) was then added to the cell lysis and the nuclei were pelleted by centrifuging at 500xg for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by dilution of the supernatant with fresh medium in a ratio of 1:1 and addition of 3 µg/mL hexadimethrine bromide (polybrene, Sigma-Aldrich). 5 µg/mL Puromycin were added and maintained to select for positively transduced cells ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Synthetic Biology 2024Quote: ... filtered spent media collected from a confluent flask) at the ratio of 1:3 to fresh medium with 25% FBS and 1% penicillin-streptomycin (Penicillin-Streptomycin, Sigma Aldrich). Confluent wells were then assessed with fluorescence microscopes ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO], 20 ng/ml bFGF [Sigma], and 20 ng/ml EGF [Sigma]) before collection for single-cell analysis ...
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bioRxiv - Immunology 2024Quote: ... we used inhibitors of ERK1/2 (Trametinib, SelleckChem, Catalog # S2673), p38 (Losmapimod, Selleckchem Catalog #S7215), NF-kB (BAY11-7082, Catalog # S2913) and JNK inhibitor (Millipore-Sigma Catalog # CAS 129-56-6) 2 hr before stimulation with R848 or Poly I:C until sample collection at 8 or 24hrs for mRNA or protein levels of antiviral and inflammatory cytokines and chemokines.
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bioRxiv - Cancer Biology 2021Quote: ... Crosslinked pellets were thawed and lysed on ice for 30 min in ChIP harvest buffer (12 mM Tris-Cl, 1 × PBS, 6 mM EDTA, 0.5% SDS) with protease inhibitors (Sigma). Lysed cells were sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... Cross-linked pellets were thawed and lysed on ice for 30 min in ChIP harvest buffer (12 mM Tris-Cl, 1 × PBS, 6 mM EDTA, 0.5% SDS) with protease inhibitors (Sigma). Lysed cells were sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Biophysics 2020Quote: Transfection of 1 × 105 293T cells per well of a 6-well plate was carried out using GeneJuice (Novagen) transfection reagent as per manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... and embryos from natural spawning were injected with up to 600pg RNA in phenol red (P0290, Sigma, 1:6). Injected embryos were maintained at 28.5°C in egg water and collected at 26hr for histology ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was washed in a pre-hybridization solution (6 x SSC, 1 x Denhardt’s, 100 μg/ml denatured Herring Sperm DNA (Sigma) and 0.5 % SDS ...
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bioRxiv - Developmental Biology 2022Quote: ... and 37°C with daily medium change and split twice a week in a 1:6 ratio using 0.05% Trypsin-EDTA (Sigma).
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bioRxiv - Cancer Biology 2019Quote: ... Cross-linked pellets were thawed and lysed on ice for 30 min in ChIP harvest buffer (12 mM Tris-Cl, 1 × PBS, 6 mM EDTA, 0.5% SDS) with protease inhibitors (Sigma). Lysed cells were sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Neuroscience 2021Quote: ... band power values in each frequency band (Delta:1-4Hz; Theta:6-9.5Hz; Sigma:10-16Hz; Gamma:30-60Hz) were divided by a normalization factor that corresponds to the sum of the power across all the frequencies according to the experimental condition ...
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bioRxiv - Plant Biology 2020Quote: ... and infiltrated with a solution containing 240 mM sorbitol and 6 mg.L-1 Lucifer Yellow CH dipotassium salt (LYCH, Sigma), which is used as a tracer to normalize apoplastic fluids (Derrick et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich) and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... 10 µL of the HDX reaction were removed and quenched by diluting 1:6 into 0.75% formic acid (FA, Sigma), and digested for two min with porcine pepsin (0.25 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... In the tMCAO study the brains were cut in 6 coronal sections and stained with 1% 2,3,5-Triphenyltetrazolium Chloride (TTC) (Sigma-Aldrich) in PBS solution for 5 minutes at 37 °C in agitation before dissection of contralateral and peri-ischemic cortical regions ...
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bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich, cat# ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL of this dilution was then added to 90 μL of buffered D2O (prepared 5 mL with 4.5 mL of D2O and 0.5 mL of 10x protein dilution buffer and 0.2 μg/mL of peptide standard Glu-1-Fibrino peptide (CAS: 103213-49-6, Sigma)) to initiate deuteration at 22 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg viral plasmid were diluted in 6 μL X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich, 6365787001) and 150 μL DMEM and incubated at room temperature for 15 min before being added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... A 30-min incubation of PBS supplemented with 1 µg/mL of 4′,6-diamidino2-phenylindole (DAPI) (Sigma-Aldrich) was performed ...
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bioRxiv - Pathology 2024Quote: ... of mosquito EV-lipid extract or of EV-lipid fraction 6 was treated with 1 U of SMase (Sigma) for 1h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in a 24-well plate (500’000 cells/well) in complete IMDM supplemented with 6 ng/ml IL-6 (Sigma), 6 ng/ml IL-3 (eBioscience ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatants were incubated with 6 µl anti-HA (12CA5, in-house preparation) or 6 µl anti-T7 (69522, Millipore) and protein G-dynabeads (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Loaded PDMS chambers were incubated for 30 minutes at 37°C and after polymerization muscle bundles were kept in normal proliferation medium containing 1.5 mg/ml 6-aminocaproic acid (6-ACA) (Sigma). After 2 days ...
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bioRxiv - Neuroscience 2022Quote: Partial bilateral neostriatal dopamine lesions were made by injecting 6-OHDA (6 μg/μL, Sigma-Aldrich, St. Louis, MO) dissolved in ascorbic saline (de-ionized water containing 0.9% NaCl and 0.1% ascorbic acid ...
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bioRxiv - Cell Biology 2022Quote: C57Bl/6 mice (6 weeks-old) were treated with an ip injection of 1500 mg TAA/kg (Sigma-Aldrich) to induce ALF ...
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bioRxiv - Neuroscience 2024Quote: ... each mouse received a bilateral injection of 1.25 μl of 6-hydroxydopamine hydrochloride (6-OHDA, Sigma-Aldrich, 4μg/μl) or vehicle (0.9 % NaCl + Ascorbic Acid 0.02% ...
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bioRxiv - Immunology 2024Quote: ... BEAS-2B.MR1-GFP cells were incubated with 94 μM 6-formylpterin (6-FP, Schircks) or the equivalent volume of 0.01 M NaOH (Sigma) overnight at 37°C at 5e4/well in a 24-well plate ...
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bioRxiv - Developmental Biology 2023Quote: Pups were injected once daily intraperitoneally with 6-hydroxydopamine hydrochloride (6-OHDA) 50ug/g of body weight (Sigma H4381) from post-natal day 0 to 5 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA rats received 2.3 μL bilateral injections of 6-OHDA hydrobromide (6 μg combined with ascorbic acid dissolved in 2.3 μL sterile 0.9% NaCl, Sigma Aldrich) in the SNc in each cerebral hemisphere ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Biophysics 2019Quote: ... 3 μL benzonase (Novagen) and sonicated ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μM CHIR99021 (Sigma), 1 μM PD0325901 (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... 3 % donkey serum (Millipore) and 0.5 % Triton X-100 in PBS at RT for 3 h ...
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bioRxiv - Developmental Biology 2020Quote: ... 3) Progesterone (Sigma P8783) – 0.02 µg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: Nutlin-3 (Sigma, N6287) was dissolved in corn oil (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... 3% donkey serum (Millipore), and 0.2% Triton X-100 in PBS ...
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bioRxiv - Genetics 2019Quote: ... 3 mM ATP (Sigma), 1 mM CaCl2 ...
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bioRxiv - Cancer Biology 2019Quote: ... pHistone 3 S10 (Millipore), pp53 S15 (Cell Signaling) ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3% sucrose (Sigma-Aldrich), 0.8% Phytoagar (Duchefa) ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3% sucrose (Sigma-Aldrich), 0.8% Phytoagar (Duchefa) ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3% sucrose (Sigma-Aldrich), 0.8% Phytoagar (Duchefa) ...