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Citations for Millipore Sigma :
4351 - 4400 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were then isolated with CD14+ microbeads (MACS Miltenyi) and incubated for 6 days in RPMI/10%FBS and 1% penicillin/streptomycin solution (Sigma) supplemented with 50 ng/mL human GM-CSF (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1-1.2x106 cells/ml onto 6-well plates treated with poly-D-lysine (EMD Millipore). Cells were cultured in Neurobasal A medium supplemented with 2% B27 ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... were treated with 50 µL of a 1mg/ml Concanavalin A (prepared in 10 mM sodium phosphate buffer pH 6, 10 mM CaCl2 ,1 mM MnCl2, 0.01% NaN3, Catalog C2010, Sigma Aldrich) solution ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM tris(2-carboxyethyl)phosphine (TCEP)] containing 1 SIGMAFAST protease inhibitor EDTA-free cocktail tablet (Sigma). Due to the presence of two remaining cysteines in PelXC232S ...
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bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
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bioRxiv - Plant Biology 2019Quote: ... the T1 seed selection was done on 1/2 MS medium + 10 mgL-1 Glufosinate-ammonium (Sigma-Aldrich). For construct containing the FASTR screenable marker ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 µl of SUV TBS solution (1:1 ratio of SUV and 2× TBS solution, TBS form Sigma) was placed in each hole of the device ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 plus 5 μM Blebbistatin (Bleb, B0560, Sigma), pelleted at 150g for 5 minutes ...
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bioRxiv - Physiology 2019Quote: ... The powdered food mix was added in a 1:1 ratio (grams:mL) to a 2% agar (Sigma #A1296) solution in water before solidification to a semi-solid consistency that lessens the potential for hoarding of food in group housing of mice and increases accuracy of measuring food consumption as large chunks can’t be taken at one time and the food doesn’t crumble like with standard rodent chows ...
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bioRxiv - Genomics 2021Quote: ... 10 mM MgCl2,10 mM Tris pH 7.4, 1 mM CaCl2) with increasing amounts (0, 0.5, 1, 2, 5, 7.5, 10 U) of MNase (Sigma) at 30°C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DMEM without serum contained 1 µg of L-1- tosylamide-2-phenylethyl chloromethyl ketone-treated trypsin (TPCK; Sigma) for activation of the virus infectivity ...
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bioRxiv - Cancer Biology 2023Quote: ... the viral medium was removed and the wells overlaid with a 1:1 solution of 2% agar (Sigma) and 2X DMEM containing 5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: LL-341070 was provided by Autobahn Therapeutics as a solution in 1% 1-methyl-2-pyrrolidone (Sigma 270458) and 1% Kolliphor HS 15 (Sigma 42966 ...
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bioRxiv - Physiology 2023Quote: ... the gonads were incubated overnight in the dark at 4 °C with secondary goat anti-rabbit IRDye 680RD-conjugated antibody (LiCor) 1:1000 in PBST/0.1% BSA and 1 ng/µl 4,6-diamidine-2-phenylindole (DAPI, Sigma). The gonads were mounted on 2% agarose pads in Fluoromount-G (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation of secondary antibodies (1:500) for 2 h and DAPI (1:10,000, D9542, Sigma-Aldrich) in PBS for 5 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Bioengineering 2023Quote: ... b-mercaptoethanol and 1000 U ml-1 LIF) and 2 ug ml-1 doxycycline (cat. 33429 Sigma-Aldrich). MEFs were reprogrammed in the presence of doxycycline for 12 days and without doxycycline for the last 5 days ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 with 5 µM Blebbistatin (Bleb, B0560, Sigma), and pelleted at 150 g for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The yeasts were grown in yeast extract (1%, w/v) peptone (2%, w/v) glucose (2%, w/v) (YPD) media supplemented with 50mg/L ampicillin (Sigma-Aldrich, 69534). The strains were grown in 250 mL Erlenmeyer flasks containing 100 mL of YPD ...
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bioRxiv - Molecular Biology 2020Quote: ... sections of mouse heart were perfused for 1-2 min with 2ml of 2% TTC (Sigma-Aldrich Co., St. Louis, MO, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl) piperazin-1-yl) ethane sulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO), 24 mM sodium bicarbonate ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast cells were grown in rich medium (YPD; 1% yeast extract [Difco, 10215203], 2% peptone [Difco, 211705], and 2% glucose [wt/vol]) (Sigma-Aldrich, G7021) on an orbital shaker (200 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... were resuspended 2 mL of viral particle supernatant for E:T of 2:1 with 8 μg/mL of Polybrene (Millipore Sigma, Burlington, MA), maintained in at 37°C for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates were extracted from the hippocampi or cortices of adult mice and dissected in ice-cold PBS containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and Mini-Complete Protease Inhibitor Cocktail (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (2 months old) were transduced with 250 µl of Auto-hLAG3 LV and 3 µg/ml of polybrene (Sigma-Aldrich #TR-1003-G) per well of a 6 well plate ...
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bioRxiv - Bioengineering 2020Quote: ... Aortas were then washed in 78% methanol for 5 minutes under gentle movement for a total for 3 times before incubating in a 2% ORO solution (Sigma-Aldrich, St Louis, MO) for 60 minutes ...
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bioRxiv - Neuroscience 2020Quote: Protein lysates were extracted from the hippocampi or cortices of adult mice and dissected in ice-cold PBS containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and Mini-Complete Protease Inhibitor Cocktail (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with lysis buffer (RIPA buffer, 1x Roche Complete Protease Inhibitor cocktail, 1X Sigma Phosphatase Inhibitor Cocktail 2, and 1X Sigma Phosphatase Inhibitor Cocktail 3), and incubated on ice for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: Alveolar organoids were removed from Matrigel by mechanical dissociation with a cut pipette tip or retrieved from HA hydrogels with 2% hyaluronidase (Sigma Catalog #37326-33-3) for 30 minutes at 37°C followed by mechanical dissociation with a pipette tip ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine, Sigma-Aldrich, Cat# U3003-5G). 120 µL of cell suspension was carefully plated onto each coverslip and allowed to adhere for 2 hours at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... an isolated infrarenal aortic segment of C57BL/6J mice was perfused with sterile isotonic saline containing type I PPE (2-3 U/ml, E1250, Sigma-Aldrich, Burlington, MA, USA) for 5 min under pressure (120 mmHg ...
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bioRxiv - Microbiology 2023Quote: ... freshly drawn whole blood was mixed with an equal volume of a solution containing 3% dextran (Sigma-Aldrich, ACS Cas.# 9004-54-2, Denmark) and 1.8% sodium chloride (Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... a 5 µL drop of the formulation was placed on a glass slide that was silanized by liquid phase deposition in a solution of 2% 3-(trimethoxysilyl)propyl methacrylate (cat. #M6514, Sigma-Aldrich, Oakville, Ontario, Canada) prepared in toluene (cat ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 3 (10 mg/kg) intraperitoneally (i.p.) 30 min prior to injection of zymosan (Sigma-Aldrich; 2 mg/ml in saline, 0.5 ml, i.p.). Mice were sacrificed (by CO2 inhalation ...
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bioRxiv - Cell Biology 2024Quote: ... Phase separation of lipids from the serum was performed using an organic phase of diisopropyl ether: n-butanol (3:2; Sigma Aldrich, 296856, B7906, respectively). Organic and aqueous phases were allowed to mix at room temperature with continuous stirring in the dark for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were stained for 2 h in PBS containing 2 µg/ml DAPI and mounted in a 1:1 mix of glycerol and water containing 1 % DABCO antifade (Sigma-Aldrich, USA) for confocal imaging using a Zeiss (Cambridge ...
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bioRxiv - Microbiology 2020Quote: ... 49 with 1.5% weight vol−1 agar supplemented with 2 mg mL−1 5-Fluoroorotic acid (USBiological, USA) and 5 µg mL−1 uracil (Sigma–Aldrich USA) in order to select for plasmid excision ...
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bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
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bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO-Puro shHDAC4 1 (TRCN0000314667) and 2 (TRCN000004832) were obtained from Sigma-Aldrich. pWZL-Hygro-HDAC4PAM (V31L or P16A) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM EDTA and 1% NP40) supplemented with Protease Inhibitor Cocktail (Sigma-Aldrich). Proteins (25-50µg ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 × 100 U/mL penicillin-streptomycin and 0.05 mM 2-Mercaptoethanol (Sigma-Aldrich). At 168 h ...
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bioRxiv - Cell Biology 2020Quote: ... cells were blocked for 1 h in 2% bovine serum albumin (BSA) (Sigma)/PBS solution at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were diluted 1:2 for RT-qPCR using SYBR Green Mastermix (Sigma) and primers detailed in Table 3.
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...