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Citations for Millipore Sigma :
4051 - 4100 of 4323 citations for Recombinant Human HAPLN1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Immunofluorescence was performed using an automatic liquid handling dispenser (Personal Pipetter, ApricotDesigns) using Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...
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bioRxiv - Cell Biology 2022Quote: Transient knockdown of human ANXA1 or LGN in MCF-10A cells was achieved by transfection of MISSION® Predesigned siRNAs from Sigma. The following products were used ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were transferred to a new dish on ice and minced into ∼1mm3 pieces followed by enzymatic digestion using a cocktail for human tissues composed of Collagenase A (10103578001, Sigma Aldrich), Collagenase IV (NC9836075 ...
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bioRxiv - Bioengineering 2024Quote: ... Rat tail collagen type I solution in 0.6% acetic acid (First Link, UK) was combined with human AB serum (HS; Sigma-Aldrich, H4522), 10x DMEM ...
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bioRxiv - Bioengineering 2024Quote: ... and Endothelin-1) using a fluorometric MILLIPLEX ® Human Angiogenesis/Growth Factor Magnetic Bead Panel Multiplex Assay (Sigma-Aldrich, Cat # HAGP1MAG). Each plate was run according to the manufacturer’s protocol using 25 µL sample per well.
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bioRxiv - Cell Biology 2024Quote: ... they were covered with a 100 µl drop of a solution of 20 µg/ml fibronectin (fibronectin from human plasma, Sigma Aldrich) and 15 µg/ml fibrinogen-Alexa488 conjugate (F13191 ...
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bioRxiv - Molecular Biology 2024Quote: ... Spinfection plates were pre-coated using 500 uL of PBS with 10 uG/mL of purified human plasma fibronectin (Sigma-Aldrich) and incubated at 37°C several hours prior to transduction ...
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bioRxiv - Cell Biology 2024Quote: ... U-2 OS human cell line (ATCC HTB-96) was transfected with the GFP-tau vector using the X-tremeGENE HP reagent (Sigma Aldrich) and then selected with 200 µg/mL zeocin ...
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... The tissues were cryopreserved in cryo-medium (40% PBS; 50% Human Serum, Sigma-Aldrich, St. Louis, MO, USA; and 10% DMSO) and stored at -150 °C for further analysis.
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
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bioRxiv - Cell Biology 2023Quote: IMR-90 normal human fibroblasts (ATCC, CCL-186) and HEK293T cells were cultured in DMEM (Sigma-Aldrich, D5796; Wako, 044-29765) supplemented with 10% FBS (BioWest ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were measured using the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex-Immunology Multiplex Assay (Millipore) according to the manufacturer’s recommendations and analysis was done using the Bio-Plex Manager 6.1 software based on the standard curve.
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were harvested and maintained in T cell medium containing Roswell Park Memorial Institute 1640 Medium (RPMI) supplemented with 100 U/ml Penicillin-100ug/ml Streptomycin and 10% human serum (HS, Sigma-Aldrich), IL-7 (ImmunoTools ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451 ...
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bioRxiv - Cell Biology 2023Quote: ... basal media was removed from the cultures and media containing 500 U/mL human IFNβ (cat. no. IF014, lot 3481402, Millipore Sigma) was added to the basal chamber ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 500k Day 25 hiPSC-CMs and 100k human Hs27a stromal cells were mixed with 3 U/mL thrombin from bovine plasma (Sigma, T4648) and 5 mg/mL bovine fibrinogen (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Samples of gB-SE or gD-SE immobilized on Strep-Tactin Sepharose beads were incubated with 1 mL of diluted human γ-globulins (G4386; Sigma) (0.082 and 3.04 mg/mL in medium 199 containing 1% FCS and ADCC assay buffer for neutralization assays and ADCC assays ...
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bioRxiv - Microbiology 2022Quote: Human foreskin fibroblast (HFF; ATCC® designation SCRC-1041 ™) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Sigma, D6546) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... the wells were filled with 200 µl fresh M63 media with or without 3 µM of human transferrin (Sigma-Aldrich #T1147) and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... We confirmed the specificity of our IgA ELISA and non-cross reactivity toward IgG using purified human IgG and IgA (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... Cytokine levels in supernatants were analyzed by 38 plex MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore HCYTMAG-60K-PX38) adapted for use with 96-well DropArray plates and DropArray LT210 washing station (Curiox Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... The human embryonic tissues were dissociated for 50 minutes at 37°C with 10 mg/ml Collagenase/Dispase (Sigma Aldrich, 10269638001) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Physiology 2023Quote: Protein lysates were prepared from primary lung endothelial cells and human umbilical vein endothelial cells using ice cold RIPA buffer (Sigma Aldrich) with proteases and phosphatase inhibitor cocktails (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human CD56 Positive Selection Kit II) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... coating for 5 min at 22 °C protected from light with sterile filtered DOPA in pH 10 [10 mM] Tris buffer DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 µg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 °C to generate an expected fibronectin coating density of 6 μM/cm2.
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bioRxiv - Cell Biology 2023Quote: ... Despite the successful reprogramming of a human neonatal foreskin or adult skin (normal) fibroblast line purchased from Sigma (Cat 106-05A), multiple attempts to reprogram the Nigerian fibroblasts failed for reasons that are not entirely clear ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... were superovulated by injecting them with pregnant mare serum gonadotropin (5 IU, Prospec) and human chorionic gonadotropin hormone (5 IU, Sigma-Aldrich) intraperitoneally at 48-h intervals ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C and 5% CO2. Human BLaER1 cells (a kind gift of Thomas Graf) (Rapino et al. 2013) cells were grown in RPMI (Sigma-Aldrich) supplemented with 10% FCS and 2 mM L-glutamine at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: The MISSION® pLKO.1-puro Non-Target shRNA (scr) and the shRNA against the UTR of human ZIP11 gene (5’-TCCTGATTGACTCTGATTATA-3’, Cat. TRCN0000434903) were from Sigma-Aldrich. The mammalian gene expression vector pLV[Exp]-EGFP/Neo-Ef1A (pLV ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.75 IU/kg of human insulin (Actrapid 100 IU/mL, Novo Nordisk) for insulin tolerance tests (ITT) or 2 g/kg pyruvate (Sigma-Aldrich) for pyruvate tolerance tests (PTT ...
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bioRxiv - Immunology 2023Quote: ... 100,000 T84 cells per well were seeded on glass bottom 8-well chamber slides coated with 2.5% human collagen (Sigma #C5533-5MG) diluted in water ...
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bioRxiv - Microbiology 2023Quote: ... Presence or absence of a ZCCHC3 specific band with each clone was determined by western blotting using an anti-human ZCCHC3 antibody (SAB1408147, Sigma-Aldrich).
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bioRxiv - Bioengineering 2023Quote: ... Human mammary epithelial cell line (MCF10A) was cultured in DMEM/F12 (Cytivia: SH30023.01) with 5% horse serum (Sigma Aldrich: H1138-500ML), 0.2% Normocin ...
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bioRxiv - Biophysics 2023Quote: Human Serum from human male AB plasma (H6914, 1st lot SLCH8785, 2nd lot SLCJ3635) and (H4522, lot SLCK9619) were purchased from Sigma-Aldrich. Serum samples from healthy individuals and cancer patients ...
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bioRxiv - Cell Biology 2022Quote: Ibidi µ-Slide I slides (#80106, IbiTreat) were incubated with 10 μg/mL fibronectin (from human plasma, #2006, Sigma-Aldrich Inc.) in PBS at room temperature for 1.5 hr ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and and CD44 (SAB1405590) (mouse) at 1:400 dilution for zebrafish tissue and 1:50 dilution for human tissue (Sigma Aldrich). The following secondary antibodies were used ...
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bioRxiv - Developmental Biology 2023Quote: ... the caudal epididymis was dissected and the epididymal sperm was collected on a needle and capacitated in EmbryoMax Human Tubal Fluid media (HTF, EMD Millipore) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: Serum autoantibodies were quantified using the Luminex xMAP platform (Luminex) with the MILLIPLEX MAP Human Autoimmune Autoantibody Panel (HAIAB-10K, Millipore Sigma) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... purified human neutrophil or mouse BM-derived neutrophils were stimulated with 10-7M N-formyl-Met-Leu-Phe (fMLF; Sigma-Aldrich) or 1 μM WKYMV (Phoenix Pharmaceuticals ...
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bioRxiv - Cell Biology 2023Quote: Round 25mm coverslips were squeaky-cleaned (56) and plasma cleaned before coating with 20µg/mL Human Plasma Fibronectin (EMD Millipore #FC010) at 37C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Systems Biology 2024Quote: The MIA PaCa-2 pancreatic cancer and HEK 293 human embryonic kidney were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma Aldrich, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...