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Citations for Millipore Sigma :
3901 - 3950 of 4323 citations for Recombinant Human HAPLN1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... were isolated from PBMCs using anti-CD14-antibody-coated magnetic beads (Miltenyi) and cultured in RPMI1640 supplemented with 10% human AB serum (Sigma), pen/strep ...
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bioRxiv - Physiology 2023Quote: Glycerol stocks of pLKO.1-puro lentiviral plasmid vectors containing shRNA targeting human Nup93 (NM_014669) and an empty vector insert (shEmpty) were purchased from Sigma-Aldrich. Lentivirus expressing shNup93 or shEmpty were generated by PEI-mediated co-transfection of pLKO.1-puro ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... The wells were washed with PBS-0.1% Tween 20 and then incubated with 100 μl of 100 μg/well of biotinylated rabbit anti-human C1q (Sigma) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Synthetic Biology 2023Quote: Stable cell lines expressing human or mouse PRLR were generated by lentiviral transduction followed by 0.7 ug/mL puromycin selection (Sigma; P9620). 12ug of each pTwist Lenti Puro SFFV WPRE lentiviral construct encoding either human or mouse PRLR were co-transfected with 9ug of psPAX2 (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... a spectrophotometric stop reaction was used to calculate the activity of human salivary α-amylase (Sigma-Aldrich, cat. No. 1031) using Equation 1 (b = intercept of linear regression ...
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used in this study were: polyclonal rabbit anti-human HINT1 antibody (1:1000, Sigma, San Luis, MO, USA), and to demonstrate equal loading mouse monoclonal anti-β-actin antibody (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... a pellet of neutrophils was resuspended in 800 μL HBSS with calcium and magnesium and 200 μL human serum (Sigma). Each 10 mL lithium-heparin coated tube of blood drawn yielded 1 mL of neutrophil-only solution ...
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bioRxiv - Developmental Biology 2023Quote: ... Four- to five-week-old C57Bl/6 females were superovulated and MII oocytes collected in fertilisation media (Human Tubal Fluid (HTF, MR-070, Sigma) containing 4 mg/ml BSA and 25mM L-Glutathione 1 (GSH)) ...
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bioRxiv - Cancer Biology 2023Quote: ... and RANTES were simultaneously quantified from the plasma collected from the BM of patients enrolled in the clinical trials using the MILLIPLEX Human Cytokine/Chemokine/Growth Factor Panel A (HCYTA-60K-PX38, Millipore) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... Polyacrylamide gels were then immediatly coupled to either human plasma fibronectin (1 mg/mL overnight at 37◦C; FC010, Millipore) or ICAM-1 (0.1 mg/mL Recombinant Protein A (Novex ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibod-ies were used for western blots of human cell extracts: Mouse anti-FLAG (Sigma F1804, 1:1000); Mouse anti-GAPDH (Proteintech 60004-1-Ig ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cancer Biology 2024Quote: ... CWR22 (primary prostate tumour) human prostate cancer cell lines were obtained from ATCC and grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: The cortex tissue samples from mice or human were lysed in PBS along with PDE inhibitor (IBMX) and PIC (SIGMA) with homogenizer followed by centrifugation at 12500 rpm for 20min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... under air-liquid interface conditions in Greens+ keratinocyte proliferation media (Greens media supplemented with 10 ng/mL human EGF (Sigma)) ...
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bioRxiv - Immunology 2024Quote: ... and perfusate levels of 71 analytes using two separate panels as per the manufacturer’s instructions for use: (1) the Human Cytokine 48-Plex Discovery Assay® (HD48; Millipore MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel Immunology Multiplex Assay ...
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bioRxiv - Microbiology 2024Quote: Human fecal samples (10-20 mg) were resuspended in supplemented Brain Heart Infusion (BHI, Sigma-Aldrich, St. Quentin Fallavier, France) as described in(Motta et al. ...
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bioRxiv - Neuroscience 2024Quote: Total protein was extracted from 20-30 mg human frontal cortex using RIPA buffer (PierceTM RIPA Buffer, ThermoScientific, 89901) supplemented with 0.05% CHAPS (Sigma, C3023), 1X complete protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown to 70-80% confluency on surfaces were coated with 10μg/ml of purified human fibronectin (SIGMA-Aldrich). Orbital shear stress was applied to the cells for 18-24 h at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... albumin from chicken egg white (44 kDa) and γ-globulins from human blood (160 kDa) were purchased from Sigma-Aldrich.
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bioRxiv - Physiology 2024Quote: ... Protein lysates were prepared from human RV tissue using modified RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Sigma). Proteins were diluted in 4x Laemmli sample buffer ...
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bioRxiv - Physiology 2024Quote: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141) ...
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bioRxiv - Cell Biology 2024Quote: ... the chips were incubated with 0.1% (m/v) gelatin combined with 20 µg/ml human plasma fibronectin (Sigma-Aldrich, 341635) in PBS at 37°C for 1 hour ...
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bioRxiv - Immunology 2024Quote: Single cell solution was obtained from human normal donor and IPF lungs after enzymatic digestion with 10X Liberase (Sigma, 5401127001) and DNaseI (9003-98-9 ...
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bioRxiv - Immunology 2024Quote: BMMCs were sensitized for Ag stimulation with dinitrophenol (DNP) coupled to human serum albumin (HSA) (DNP-HSA, Sigma-Aldrich, #A6661) by pre-loading the FcεRI with 0.15 µg/ml IgE (clone SPE-7 ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: Human HEK293T cell line was grown in Dulbecco’s Modified Eagle’s Medium containing high glucose with HEPES modification (Sigma-Aldrich®, D6171), supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Molecular Biology 2021Quote: Murine or human differentiated brown adipocytes were fixed with 4% PFA and stained with 0.3% Oil Red O solution (Sigma-Aldrich, O0625). The images were observed under a Nikon microscope.
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bioRxiv - Biochemistry 2020Quote: ... The following compounds were selected from the “in-human” collection docking screen and purchased from different vendors: Pterin (Sigma-Aldrich, P1132), Verdiperstat (MedChem Express ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Immunology 2019Quote: Blood derived from healthy human volunteers was collected into citrate tubes (Becton Dickinson) and layered into Ficoll-Paque Plus (Sigma-Aldrich). Samples were centrifuged at 1440 rpm in a swinging bucket centrifuge for 20 min at 23°C without braking ...
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bioRxiv - Biochemistry 2019Quote: ... Cohn fraction IV (Product # G3637) and human whole serum (sterile-filtered from male AB plasma, Product # H4522) were purchased from Sigma-Aldrich, St ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Immunology 2019Quote: ... Cytokine levels in restimulated T cell cultures and SLE patient plasma samples was measured using Milliplex® MAG Human Cytokine/Chemokine Immunoassays (Millipore).
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bioRxiv - Neuroscience 2019Quote: ... transverse paraffin sections of spinal cord mounted on positive charged glass slides were processed for double immunohistochemistry with specific antibodies for human neural stem cells (Sigma-Aldrich) and examined under a fluorescence microscope ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Immunology 2019Quote: ... PerioPaper strips were centrifuged at 3000 × g for 15 min at 4°C in PBS elution for the dosage of the tissue destruction and bone resorption markers by Multiplex (Human MAP, Millipore, USA).
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bioRxiv - Biochemistry 2019Quote: ... and eluted by competitive elution in Flag Wash Buffer supplemented to 200 ug/mL Flag peptide and 250 ug/mL human insulin as a carrier (Sigma-Aldrich). The protein was then passed over a 2 mL amylose column (NEB ...