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Citations for Millipore Sigma :
4051 - 4100 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and 1 cOmplete protease inhibitor tablet per 1 L of culture (Sigma Aldrich), and cells were lysed by sonication ...
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bioRxiv - Cell Biology 2024Quote: ... Par3 (IF: 1:200, WB: 1:1000, 07-330, EMD Millipore, Burlington, MA), and beta-actin (IF ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were diluted 1:1 with 100 mM triethylammonium bicarbonate (TEAB, Sigma, T7408), reducing the urea concentration to 4 M ...
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bioRxiv - Neuroscience 2024Quote: ... mouse IgG1 anti-Pan Nav 1 (1:100; N419/78; Millipore, MA, USA), mouse IgG1 anti-Nav 1.6 (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-INCENP (Millipore, 05-940, 1:250-IF 1:1000-WB) and Rat anti-Tubulin (EMD Millipore ...
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bioRxiv - Systems Biology 2024Quote: ... and a 1:1 mixture of 0.75 mM oleic acid (Sigma, Cat. # 364525) and 0.75 mM palmitic acid (Sigma ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:1000 in blocking buffer (PBS 1X containing 1% BSA (Sigma Aldrich) and 0.1% Tween 20) ...
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bioRxiv - Microbiology 2024Quote: ... A 1:1 mixture was added to a sterile 0.2 µm filter (Millipore) on an LB plate supplemented with DAP and incubated at 37°C for 3 hrs ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% P/S and 1 μg/ml TPCK trypsin (Sigma, T1426). Centrifugation (2,500 ×g ...
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bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
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bioRxiv - Plant Biology 2021Quote: ... was analyzed by extracting the gDNA with either the innuPREP Plant DNA Kit (Analytik Jena; Jena, Germany; T0) or the REDExtract-N-AMP plant Kit (Sigma Aldrich; T1, T2). The transgene was detected by amplifying the Ocs terminator of the hygromycin resistance cassette (Table S3).
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bioRxiv - Bioengineering 2022Quote: ... the lipid oxidation and superoxide level assays were conducted according to manufacturer’s specifications (Lipid peroxidation assay kit, Sigma Aldrich Cat#MAK085; Fluorimtric hydrogen peroxide assay kit, Sigma Aldrich Cat#MAK165). Total GSH level of liver tissue was measured using a glutathione colorimetric detection kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... ab195352, Abcam; anti-METTL14: 1:200, HPA038002, Sigma-Aldrich; anti-WTAP: 1:500, ab195380, Abcam; anti-Flag: 1:200, F3165, Sigma-Aldrich) at 4 overnight ...
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bioRxiv - Plant Biology 2019Quote: ... IP1 and IP2 buffers were supplemented with proteinase inhibitor cocktail (1 mM PMSF, 1 mg/mL leupeptin [Sigma-Aldrich L2023], 1 mg/mL aprotinin [Sigma-Aldrich A6191] ...
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bioRxiv - Neuroscience 2019Quote: ... guinea pig anti-Synapsin 1 (1:500, SySy #106004), mouse anti-SMI-3 (1:2000, Covance #SMI-312R) and rabbit anti-GABA (1:1000, Sigma Aldrich #A2052). After washing with PBS ...
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bioRxiv - Immunology 2019Quote: ... were cut and stained with primary antibodies (rabbit anti-CGRP, Millipore, PC205L, 1:500; guinea-pig anti-TRPV1, Millipore, AB5566, 1:1,000; chicken anti-Neurofilament 200, Millipore, AB5539, 1:500). Sections were washed in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... and VGT3 (1 μg/ml) (J. Somogyi et al., 2004), Sst (Penisula lab., T-4103.0050: 1/2000) or PV (Sigma: P3088, 1/2000).
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bioRxiv - Systems Biology 2020Quote: ... rabbit anti-PDGFRα (1:400, gift from Prof Stallcup); goat anti-PDGFRα (1:200, R&D Systems; mouse anti-APC (CC1; 1:300, Millipore); rabbit anti-GFAP (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were used to detect the following proteins: GFP (1:200, CST, cat. # 2956,), peripherin (1:1000; Novus, cat. # NBP1-05423, NF200 (1:500, Millipore, cat # MAB5266). Primary antibodies were applied and incubated at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h at RT followed by nuclear counterstaining with DAPI (1 μg/ml in 1 x PBS; Sigma-Aldrich, Germany) for 10 min at RT ...
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bioRxiv - Plant Biology 2022Quote: ... Inoculum was prepared to contain approximately 8,000 mycelium fragments ml-1 in sterile dH2O with 5 g l-1 glucose and 150 µl l-1 Tween 20 (Sigma-Aldrich, Germany). Two to four days prior to inoculation ...
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bioRxiv - Cell Biology 2022Quote: ... the PVDF membrane was blocked with PBS solution containing 5% nonfat dry milk for 1 hour before being incubated with primary antibodies : MyoD (ab203383) at 1:100,Myogenin (ab1835) at 1:250,MyHC (MAB4470) at 1:500 and actin (Sigma–Aldrich, A5316) at 1:800 ...
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bioRxiv - Molecular Biology 2020Quote: ... The gels were then transferred to Polyvinylidene fluoride membranes at 85V for 1 hour using 1 X NuPAGE Transfer Buffer [50 mL of 20 × 1 L Stock [81.6 g Bicine (Sigma-Aldrich, B3876-250G), 104.8 g Bis-Tris (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then diluted 1 in 4 with PBS then pelleted and re-suspended in 1 ml of PBS containing 1 % BSA (Sigma-Aldrich, #A2058). Cells were again pelleted ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were stained for 2 h in PBS containing 2 µg/ml DAPI and mounted in a 1:1 mix of glycerol and water containing 1 % DABCO antifade (Sigma-Aldrich, USA) for confocal imaging using a Zeiss (Cambridge ...
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bioRxiv - Synthetic Biology 2020Quote: ... Activity (1 U = 1 µM min-1) was calculated using a calibration curve prepared with a p-nitrophenol standard (Sigma-Aldrich, USA). Activity of the enzyme bound to the surface of each of the tested recombinants was related to the calculated activity of free purified BglC-CtDoc or BglC-AcDoc and used for quantitative comparison of the enzyme-binding capacity of the EM42 and EM371 strains displaying single-cohesin or two-cohesin scaffoldin.
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail, and 1 tablet of Millipore Sigma PhosStop (4906845001). The lysate was sonicated 2 times for 5 seconds at setting 2 using a Fisher Scientific Sonic Dismembrator Model 100 with 1 min on ice in between ...
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bioRxiv - Physiology 2021Quote: Whole liver tissues were homogenized in lysis buffer containing 20 mM Tris-HCl, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton, phosphatase (Pierce, A32957) and protease inhibitors (Sigma-Aldrich, P8340). Protein concentration was measured with BCA method (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... the primary antibody (anti-SKIV2L, Proteintech group, 11462-1-AP, 1:1000; anti-TTC37, Novus Biologicals, NBP1-93640, 1:500; anti-WDR61, Sigma-Aldrich, SAB1401852 ...
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bioRxiv - Microbiology 2020Quote: ... 49 with 1.5% weight vol−1 agar supplemented with 2 mg mL−1 5-Fluoroorotic acid (USBiological, USA) and 5 µg mL−1 uracil (Sigma–Aldrich USA) in order to select for plasmid excision ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked in 5% milk for 1 hour and then incubated with primary antibodies for Tubulin (CST, 1:5000) and acetylated Tubulin (Sigma, 1:5000) in PBS supplemented with 3% BSA and incubated overnight on a rolling platform at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then incubated with anti-CDK2 monoclonal antibody (1:100) and anti-SCP1 polyclonal antibody (NB300-229, Novus, 1:100) or anti-RAD51 polyclonal antibody (pc130, Millipore, 1:100) and anti-SYCP-3 monoclonal antibody (sc-74569 ...
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bioRxiv - Neuroscience 2023Quote: ... 4-OHT was dissolved in 100% ethanol and then mixed 1:1 with a mixture of 1 part castor oil (Sigma-Aldrich 259853) and 4 parts sunflower oil (Sigma-Aldrich S5007) ...
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bioRxiv - Neuroscience 2022Quote: ... 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1 mM sodium pervanadate, and protease inhibitor cocktail [1:100, Sigma, catalog #P8340]) at 4°C for 30 min ...
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bioRxiv - Evolutionary Biology 2022Quote: Evolution under antibiotic exposure started with a 1:1 ratio of overnight susceptible and resistant strains diluted 1:50 into fresh media supplemented with ampicillin (Millipore cat# 171254) and lysine (as indicated in the text) ...
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bioRxiv - Genetics 2024Quote: ... The primary antibodies (NF-kB, #8242 Cell Signaling, 1:1000; CD3, MCA-1477 Biorad, 1:100; acetylated Tubulin, T6793 Sigma, 1:1000; Slc12a3, HPA028748, Sigma Aldrich, 1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting collagen solution was then mixed in a 1:1 ratio with 1 U/mL bovine thrombin solution (Sigma, Cat. # T4648) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Media was then swapped to 200 ng ml-1 FGF9 (R&D, 273-F9-025) and 1 µg ml-1 heparin (Sigma Aldrich, H4784) in TeSR-E6 for 5 days to induce nephron progenitors ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed and incubated with appropriate secondary antibody diluted in TBS-T (HRP conjugated αMouse, 1:10000, CST, 7076S; HRP conjugated αRabbit, 1:10000, CST, 7074S; HRP conjugated αSheep, 1:5000, Sigma-Aldrich, A3415) for at least an hour before being washed and revealed (BioRad Clarity or Clarity Max ECL) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed 3 times with PBS and incubated with blocking solution (PBS/BSA 1%) for 1 h at 37 °C and then with the following primary antibodies: mouse anti-GFAP (1:500; Millipore, Cat. MAB360), rabbit anti-C3 (1:25 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with goat serum diluted 1:200 in PBS for one hour followed by an overnight incubation at 4°C with the primary antibody (HIF-1α, 1:200, described in32; BACH-1, 1:200, Sigma Aldrich HPA034949) Next ...
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bioRxiv - Biophysics 2024Quote: ... Panel balance and [Nucleosome : bead region] identity were confirmed using anti-dsDNA (EMD Millipore #MAB030; 1/5, 1/50 and 1/500), anti-H3.1/2 (Active Motif #61629 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with rabbit-anti-Iba-1 primary antibody (1:1000, Wako Chemicals; Cat. No) and WFA (1:400; Sigma; Cat. No) for 23 hours at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with the primary antibodies (anti-aSyn Syn-1 mouse, BD Transduction Laboratories 1:1000; anti-pS129-α-syn Rabbit Abcam Ab51253; mouse anti-β-actin, 1:10.000, Sigma Aldrich) was performed overnight at 4 °C in 5% Albumin Bovine Fraction V (BSA)/TBS-Tween ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326, 1:100; anti-IdU, BD 347580, 1:100; anti-ssDNA, Millipore MAB3034, 1:100). After washes with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... anti PMF1/NNF1 (in house made mouse affinity purified monoclonal; clone RH25-1-54, 1:1000) and anti-Tubulin (mouse monoclonal, Sigma-Aldrich; 1:10000). Quantification of protein levels from western blots was performed with the following formula ...
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bioRxiv - Biochemistry 2020Quote: ... 2 volumes of stop solution – composed of a 1:1 of acetonitrile and isopropanol with 0.1% formic acid and 1 µM telmisartan as internal standard (Sigma-Aldrich, St. Louis, MO) – were added to the assays and mixed by pipetting ...
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bioRxiv - Biophysics 2022Quote: ... the pancreas was transferred to a foil-covered glass vial containing a 1:1 solution of MeOH (100%) and BABB (1:2 (v/v) benzyl alcohol (402834-500ml, Sigma-Aldrich, Gillingham, UK) to benzyl benzoate (B6630-1L ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with Bodipy 409/505 1 ng/mL for 1 h and then incubated with DAPI (Sigma-Aldrich, 1 μg/ml) for 5 min at room temperature ...