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Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... rat polyclonal anti-Vangl2 (clone 2G4, 1:200 for Western blotting, 1:400 for immunoprecipitation, 1:500 for immunostaining, Merck Millipore, catalog no. MABN750), mouse monoclonal IgG1k anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Developmental Biology 2020Quote: ... permealized in 0.1% Triton X and 5% goat serum in PBS for 1 hr and incubated with primary antibody (anti-FLAG (Sigma, F1804, 1:300), anti-OCT4 (R&D ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4 °C with blocking solution containing the primary antibodies rabbit anti-allograft inflammatory factor 1 (Iba1, dilution 1:200; #019-19741, Fujifilm Wako, Japan) and mouse anti-β-actin (dilution 1:400, Sigma-Aldrich #A3854). Cells were then incubated for 1 hour with the secondary antibodies AF488-conjugated anti-rabbit IgG (dilution 1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... WB 1:1000), anti-ITGA5 (Cat. #: 4705, CST, WB 1:1000) and anti-β-actin (Cat. #: A2228, WB 1:10 000, Sigma-Aldrich Inc.). Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... Blocking and whole-mount antibody staining was performed in PBST 1% (1% Triton X-100 in PBS) with 10% serum (goat or donkey) and 1% Dimethyl Sulfate (DMSO, Sigma-Aldrich, Cat# D8418). Antibody incubation was done at 4ºC for 2 nights while gently shaking ...
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bioRxiv - Genomics 2024Quote: ... Cells were then washed once with 1 mL blocking buffer and finally resuspended in 1 mL blocking buffer containing 1 μg/mL DAPI (Sigma, cat. no. D8417). The cell suspension was transferred to a FACS tube for sorting ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... supplemented with commercial cocktails of protease and phosphatase inhibitors [(1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µM pepstatin, and 250 µM phenylmethylsulfonyl fluoride; Cat. No: P8340, Sigma-Aldrich, Burlington, MA); (10 mM sodium fluoride ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Plant Biology 2020Quote: ... 200 μl of N,O-Bis(trimethylsilyl)trifluoroacetamide+1% trimethylsilyl (BSTFA+1% TMCS, Sigma) were added and incubated at 110 °C for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... pH 6.9) supplemented with 1% Triton X-100 and 1% glutaraldehyde (G5882; Sigma-Aldrich) or 1% Triton X-100 and 3.2% paraformaldehyde (EM grade ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-RILPL2 (1:500, Novus) and mouse/rabbit anti-HA (1:1000, Sigma). Anti-sera raised against RILPL2 in rabbit is described in Steger et al (2017 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-phospho-S345 mouse MLKL (Millipore MABC158; clone 7C6.1; 1:2000/1:200), rabbit anti-phospho-S345 mouse MLKL (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-acetylated tubulin (6-11B-1; 1:1000 for IF; Cat#6793, Sigma), mouse monoclonal anti-glutamylated tubulin (B3 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha-tubulin (Sigma, Cat no. T5618, Clone B-5-1-2; 1:200), anti-Muc1 (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... 1% (v/v) L-Glutamine and (v/v) 1% Antibiotic-antimycotic (both Sigma-Aldrich), incubated at 37 °C in a humidified 5% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Ampicillin (100 mg ml-1) or Tetracycline (20 mg ml-1; all Sigma-Aldrich) antibiotics were added when appropriate.
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bioRxiv - Developmental Biology 2021Quote: ... 1:800 mouse anti-acetylated tubulin (clone 6-11B-1, Millipore-Sigma, cat#: MABT868), 1:600 rabbit anti-FMRFamide (Immunostar ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:800 mouse anti-acetylated tubulin (clone 6-11B-1, Millipore-Sigma, cat#: MABT868), 1:600 rabbit anti-FMRFamide (Immunostar ...
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bioRxiv - Developmental Biology 2020Quote: ... when appropriate medium was supplemented with 1 μg mL−1 puromycin (Sigma-Aldrich, P8833) for 48-72 hours or 250 μg mL−1 hygromycin B (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr at 1:500 and subsequently incubated with Hoechst 33342 (Sigma-Aldrich) and mounted with Vectorshield ...
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bioRxiv - Molecular Biology 2020Quote: ... IGF-1 (100 ng ml-1) and 2DG (5 mM, Cat#D6134, Sigma Aldrich) were diluted in differentiation medium ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were diluted 1:1 with freezing medium consisting of 20% DMSO (Sigma-Aldrich) in FBS and aliquoted for −150°C storage ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... mouse ascites MAb B-5-1-2 α-tubulin 1:200 (Sigma-Aldrich #T5168); rat TgEB1 1:100 (Chen and Gubbels ...
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bioRxiv - Cell Biology 2019Quote: ... denatured with 2.5M HCL for 1 hour and then blocked in 1% BSA (Sigma) and 0.1% Tween20 ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:50 RNAse inhibitor (promega) and 1:125 Triton X-100 10% (Sigma-Aldrich)) and immediately snap frozen in an ethanol and dry ice bath ...
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bioRxiv - Physiology 2019Quote: ... Tubules were incubated with the nuclear stain DAPI (1 μg mL−1; Sigma-Aldrich) for 1 min and in some cases Rhodamine/Alexa-633 coupled phalloidin (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti-MyoVI (1:50, 25-6791, Proteus Biosciences) Anti-Pou4f3 (1:50, HPA038215, Sigma), Anti-Espin (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... diluted 1:1000 with 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO) at 4°C overnight ...
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bioRxiv - Biophysics 2020Quote: ... and any unspecific sites blocked using 1 mg ml-1 κ-casein (#C0406 Sigma) solution ...
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bioRxiv - Neuroscience 2019Quote: Complete Freund’s Adjuvant (CFA, 20 µl, 2% in 1:1 saline) (Sigma-Aldrich, F5881) was injected into the hindpaw of mice ...
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bioRxiv - Cell Biology 2021Quote: ... we added 1 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma-Aldrich, Cat# I7018-100MG) to the cells and incubated them at 37 °C for 30 – 60 min ...
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bioRxiv - Bioengineering 2020Quote: ... The digested samples were then mixed 1:1 (v/v) with formamide (Sigma-Aldrich) and run on a denaturing PAGE-Urea gel (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: Animals were anaesthetised with a combination of urethane (1 g/kg-1 i.p., Sigma), fentanyl (0.3 mg.kg- 1 -i.p. ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-MES-1 and Calu-1 (from ATCC) were cultured in RMPI-1640 (Sigma). H23 and H838 (both from ATCC ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM histamine supplemented with 1% (v/v) mammalian protease inhibitor cocktail (Sigma-Aldrich) and solubilised with 1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse acetylated tubulin antibody was incubated at 1:1000 from Sigma (6-11B-1). Secondaries Alexa fluorophores anti-rabbit 488 and anti-mouse 647 were used at 1:1000 dilutions.
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bioRxiv - Cell Biology 2021Quote: ... For Syntenin-1 target pLKO.1 plasmids with shRNA sequences were obtained from Sigma mission library (KD1 ...
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bioRxiv - Microbiology 2020Quote: ... probed for 1 h with anti-mouse-HRP-antibody (F1804, Sigma-Aldrich; 1:50,000) diluted in 1×TBS-T buffer containing 3% bovine serum albumin ...
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bioRxiv - Microbiology 2020Quote: ... A 1:1 mixture was applied to a sterile filter (0.22 μm pore, Millipore) on an LB plate ...
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bioRxiv - Plant Biology 2021Quote: ... 1% (w/v) sucrose in 1% agar (Duchefa) and 15µM DISBAC2(3) (Sigma-Aldrich). Seedlings and staining/treatment media were then placed into a custom 3D printed chambered coverglass (24 x 60mm ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM phenylmethylsulfonyl fluoride and 1% (v/v) protease inhibitors cocktail (Sigma-Aldrich, P9599). After heating at 100°C for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 % Sodium Deoxycholate supplemented with 1 X Protease and Phosphatase inhibitor cocktails (Sigma Aldrich). The cell lysate was transferred to a pre-chilled tube and debris removed by centrifugation (5 min ...
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated for 1 h at 4°C with 1 μg streptavidin (Sigma) and then resolved in SDS-polyacrylamide gel under reducing conditions ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-α-tubulin 1:10,000 (monoclonal B-5-1-2, Sigma-Aldrich). To image the blot the secondary antibodies goat anti-rabbit IgG IRDye 800CW (LI-COR ...
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bioRxiv - Neuroscience 2020Quote: ... in 1% NHS-TPBS and then to 1:1500 ExtrAvidin-horseradish peroxidase (Sigma-Aldrich) in TPBS ...
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bioRxiv - Microbiology 2021Quote: ... then incubating with mouse anti-Ubiquitin (Sigma ST1200; 1:1000, 1 hour, 37°C), washing 3 times with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-acetylated α-tubulin clone 6-11B-1 1:100 (Sigma Aldrich) and mouse polyclonal anti-PAR2 1:100 (T ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-acetylated α-tubulin clone 6-11B-1 1:2000 (Sigma Aldrich). Bound antibodies were detected using peroxidase-labeled anti-rabbit IgG (GE Healthcare) ...