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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... empty biotin modified hydrogel spheres were modified with 1:1 volume ratio of 1 mg/ml streptavidin (Sigma-Aldrich, cat #85878) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... anti-GFP (in house made rabbit polyclonal antibody; 1:1,000-4,000) anti-Vinculin (mouse monoclonal; clone hVIN-1; Sigma-Aldrich; 1:10000), anti PMF1/NNF1 (in house made mouse affinity purified monoclonal ...
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bioRxiv - Molecular Biology 2019Quote: ... was obtained from the Swansea University culture collection and grown on Sabourand dextrose agar (SDA, 40 gL−1 D-glucose, 10 gL−1 mycological peptone, 5 gL−1 technical agar (Sigma,UK), 0.5 gL−1 chloramphenicol ...
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... A small aliquot from the final suspension was taken and diluted 1:4 in CCM and then 1:1 in Trypan Blue (T8154, Sigma-Aldrich). A live/dead cell count was determined by hemocytometer and a cell viability was assessed ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Cell culture supernatant was diluted 1:1 in IP buffer (50 mM Tris-Cl, 75 nM NaCl, 1% Igepal, protease inhibitor mixture (Sigma, 11836153001), pH 7.4 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403 ...
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bioRxiv - Physiology 2021Quote: ... anti-TRPV4 (1:200, Alomone, #ACC-034) and anti-mCherry (1:500, NOVUS, #NBP1-96752) or anti-Flag (1:1000, Sigma, #F1804), at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... IF 1:500), mouse anti-cortactin (05-180, IF 1:400), mouse anti-talin (T3287, IF 1:200) all from Sigma-Aldrich; rabbit anti-EEA1 (ALX-210-239 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA, 0.5 mM DTT, 1 mM NaF, 1 mM PMSF and 1% Protease Inhibitor Cocktail from Sigma, pH 7.4) for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... PC12 cells were incubated in serum-reduced medium (1% horse-serum, 1% FBS) supplemented with 100 ng.ml-1 ß-NGF (Sigma-aldrich) during five days on PDL coated surface.
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bioRxiv - Biochemistry 2022Quote: ... mouse anti-GBP130 (1:500), mouse anti-EXP2 (1:5000, 37), rabbit anti-Nluc (1:400, 65) and mouse anti-HA (1:500, Sigma-Aldrich). After primary antibody was removed ...
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bioRxiv - Biochemistry 2022Quote: Reporter cells were plated in triplicate at 1 × 105 cells ml−1 in media with or without 100 ng ml−1 doxycycline (dox) (Sigma-Aldrich). Every 3 d ...
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bioRxiv - Cell Biology 2022Quote: For vinculin staining hMSCs are fixed with ice cooled mixture of 1:1 (v/v) (4% PFA: permeabilizing buffer (1% Triton-X-100-Sigma Aldrich) for one minute on ice ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (α-GluA2, Merck clone 6C4, 1:200; α-GluA1, Cell signaling #13185, 1:150, α-myc, Sigma #M5546, 1:1000) were applied to neurons for 10 min at room temperature followed by a washing step in PBS supplemented with 0.1 mM CaCl2 and 1 mM MgCl2 and paraformaldehyde fixation.
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-α-tubulin (T6199; IF: 1/4000) and pAb anti-Flag-tag (F7425; IF: 1/500, WB: 1/1000) were from Sigma-Aldrich, and a pAb anti-GFP (632592 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GSTπ at 1:3000 (MBL, #311), rabbit anti-IBA1 at 1:500 (Wako, #01919741) and rabbit anti-GFAP at 1:1000 (Millipore, #OBT0030). Sections were washed three times with PBS and stained using biotinylated anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were incubated overnight with primary antibodies (Anti-DNALI1: 1: 100, Abcam, Cat No: ab87075; Anti-PACRG: 1: 200, generated by our laboratory; Anti-α-tubulin, 1:200, Sigma, Cat No ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated with the rabbit anti-NeuN (1:800, Beyotime Biotechnology) or monoclonal anti-Dicer 1(1:300, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Strains were seeded in lysogeny broth (LB, 10 g L-1 NaCl, 10 g L-1 tryptone, 5 g L-1 yeast extract, Sigma-Aldrich) at 30°C with shaking overnight ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected into 293T cells at a 1:1:1:1.6:4.6 ratio using 293 Free transfection reagent (EMD Millipore Cat #72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were co-transfected into 293T cells at a 1:1:1:1.63:4.63 ratio using 293 Free transfection reagent (EMD Millipore Cat# 72181) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were then derivatized for gas chromatography analysis using 50 µL solution of 1:1 pyridine: N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (chemicals obtained from Sigma Aldrich) and incubated at 60 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 1 ml acetone/water (1:1, Carl Roth, Karlsruhe, Germany) and 0.1 % trifluoroacetic acid (Sigma Aldrich, Dreieich, Germany). 170-200 µL DHB matrix solution was applied to the sections using a pneumatic sprayer system with a nitrogen pressure of 0.7-0.75 bar ...
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bioRxiv - Cell Biology 2020Quote: ... then plated onto LB plates containing the appropriate antibiotic (100 μg ml−1 ampicillin, 50 μg ml−1 kanamycin or 25 μg ml−1 chloramphenicol, Sigma Aldrich). Plasmid DNA was extracted from bacteria using GenElute™ Plasmid Miniprep Kit (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-βactin (AC-15, 1:20000) and mouse anti-FLAG (F-3165, 1:7000; IF 1:300) from Sigma-Aldrich; rabbit anti-TOP1 (A302-589A,1:5000) ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with goat anti-mouse antibody conjugated to alkaline phosphatase (Sigma-Aldrich; A3688-1 MI; 1:10,000 1% BSA/PBS) for 2 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH, 1:10,000 dilution, Millipore; PAFAH/PLA2G7 ...
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bioRxiv - Neuroscience 2022Quote: Small and large intestines were dissected from E14 embryos and digested with 1 mg/ml DNAse 1 (AppliChem A3778) and 1 mg/ml collagenase A (Merck Millipore 10103586001) in DMEM-F12 at 37 °C while shaking ...
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bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently these cysts were washed three times with PBS and incubated with secondary anti-rabbit IgG conjugated with Alexa flour 488 (1:300) and Calcofluor White 1:20 (CFW, 1 mg/ml, Sigma Aldrich) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were ground in an ice-cold buffer (50 mmol.L-1 TRIS, 5 mmol.L-1 EDTA, 2 mmol.L-1 DTT, protease inhibitor cocktail (Sigma, St. Louis, MO, USA)) ...
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bioRxiv - Neuroscience 2022Quote: The samples were placed ventral side up in ECi-cleared phytagel blocks (1 x 1 x 1 cm, Sigma-Aldrich, P8169). These were placed in a sample holder ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... either tarsi from all six legs or the proboscis of individual animals was stimulated three times at five seconds intervals with a drop of an appetitive solution (1 M sucrose, Fisher Chemicals, Waltham, Massachusetts, USA, CAS # 57-50-1; or 1 M D-glucose, Sigma-Aldrich, Saint Louis ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2, 1:100, Millipore; mouse anti-GRIN2BB ...
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bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...
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bioRxiv - Immunology 2023Quote: ... Staining was conducted in PBS / 1 % BSA (CHO cells and huPBMCs) or PBS / 1 % BSA / 1 % normal mouse serum (bovine cells) (Sigma-Aldrich) for 40 min at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 16uL of 10000 units mL-1 (approximately 6 mg mL-1) penicillin G and 10 mg ml-1 streptomycin solution (Sigma-Aldrich) was added to each tray to inhibit the growth of pathogens (Gangur & Marshall 2020) ...
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bioRxiv - Neuroscience 2023Quote: ... 100 mM KCl; 1% NP-40; 1 mg/ml sodium heparin; 1 mM DTT; 100 μg/ml cycloheximide [#C4859-1ML, Millipore Sigma] ...
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bioRxiv - Immunology 2023Quote: ... 18 h after cell transfer recipient mice were immunized into the right hind paw with 50 μl of a 1:1 emulsion of 1 mg/ml MOG35-55 peptide (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... stained with primary antibodies overnight (1:500 mouse anti-Tubulin β3, BioLegend; 1:500 rabbit anti-Sox11, abcam; 1:500 rabbit anti-Sox9, Sigma-Aldrich; 1:250 rabbit anti-Stat3 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were incubated with primary antibodies against fibrillin-1 (described previously, 1:1000) and fibronectin (clone FN-15, 1:1000, Millipore-Sigma) diluted in blocking buffer for 2 h at RT [31] ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% SDS (10 mM Tris-HCl, pH 8.0; 1% SDS; 1 mM Na-Orthovanadate; 1x Protease Inhibitor Cocktail (Sigma-Aldrich; P8340)) and incubated for 10 min at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with CF568/AttoN647-conjugated goat anti-mouse or anti-rabbit (1:100–1:400, Thermo Fischer Scientific) and Alexa 488/568-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 90%, 100%), incubated in acetone/Spurr resin (1:1, 30 minutes, 1:2, 30 minutes) and in Spurr resin (Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich) and 5 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% (v/v) Penicillin-Streptomycin (Pen-Strep, 100 U mL−1 and 100 μg mL−1 respectively, Sigma-Aldrich, #P4333). 3T3-L1 cells were cultured routinely in DMEM supplemented with 10% (v/v ...