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Citations for Millipore Sigma :
4051 - 4100 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS for 1 h before immunoreaction with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-GFAP (1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... After a 5 h incubation, the medium was changed to infection medium (DMEM containing Pen/Strep, 35% Bovine Serum Albumin (BSA) (Sigma), 5% NaHCO3 ...
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bioRxiv - Genetics 2023Quote: ... cells at OD600 of 1.85 in conditioned SPO (filter-sterilized SPO culture after ∼5 h in 30°C) were sonicated and transferred to a concanavalin A (Sigma) treated 96-well clear ...
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bioRxiv - Neuroscience 2022Quote: 100,000 glioma cells were plated onto circular glass coverslips (Electron Microscopy Services) pre-treated for 1 h at 37 °C with poly-L-lysine (Sigma) and then 1 h at 37 °C with 10 µg/ml natural mouse laminin (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... and subcultured for 72 h in NK MACS medium supplemented with 5% human serum male AB plasma (#H4522, Sigma-Aldrich) and human IL-2 IS (200 IU/ml ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10; anti-FLAG M2, 1:1,000 dilution, Sigma, F1804).
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bioRxiv - Bioengineering 2022Quote: ... the cells were kept at dim light (2-3 μE) for 16 h and plated on fresh TAP plates containing 10 μg/mL Paramomycin (Sigma). Plates were incubated for 10 d at 30-50 μE constant light ...
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bioRxiv - Biochemistry 2022Quote: ... and serially diluted in DMEM media and incubated in 24-well plates with a semi-confluent culture of Vero cells (for ZKV samples) for 1 h at 37°C and covered with DMEM 2% fetal bovine serum + 0.8% of methylcellulose (Sigma, M0512) overlay for 4 days at 37°C and 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2022Quote: Cells were incubated with lentiviral supernatant for 24 h in the presence of 8 μg/μl of polybrene (Sigma-Aldrich) at a multiplicity of infection >5 unless otherwise stated.
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bioRxiv - Developmental Biology 2022Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA, Sigma) for 45 min in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... sperm recovered from 12-15 week old male MF1 mice was capacitated for 2 h in a 500-μl drop of HTF medium (Sigma), layered by mineral oil (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were incubated in a rotating incubator for 1 h at 4°C with bead-conjugated FLAG (Sigma; A2220), bead-conjugated HA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... Discs were aseptically removed after 48 h and dipped briefly in sterile PBS (Sigma-Aldrich, Merck Lifesciences Ltd, Dorset, UK), to remove any planktonic bacteria after which they were placed into 3 mL of one of the test agents ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated in 300 mM sodium hydroxide (NaOH) for 1 h prior to incubation with 0.01% calcofluor white (Sigma-Aldrich) in PBS (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 24-well plates (1 x 105 cells x ml-1) and incubated for 24 h before 8 µg x ml-1 Polybrene (Sigma) was added to the cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 1 h with a 1:5000 dilution of the secondary antibody (anti-rabbit IgG horseradish peroxidise-conjugated antibody, Sigma) in antibody buffer and then washed three-times for 15 min in TTBS ...
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Specimens were then washed three times in 5 mM EDTA to deplete epithelial crypts and were digested for 1.5 h in 1 mg/ml collagenase A and DNAse I (all from Sigma-Aldrich). For enrichment of LPLs ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were stimulated 4 h at 37°C with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma), 1 μg/mL ionomycin (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... the cells were firstly cultured for 4 h in a 37°C/5% CO2 incubator with 50 ng/ml PMA (Sigma) and 1 mM ionomycin (Sigma ...
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bioRxiv - Immunology 2022Quote: ... resuspended in FACs buffer and acquired within 24 h on a Cytek Aurora flow cytometer following addition of cell counting beads (Sigma).
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bioRxiv - Microbiology 2023Quote: ... and pretreated 1 h at 37°C with either 5 mM Methyl-β-cyclodextrin (MBCD) (Sigma Aldrich, stock in ddH2O) in OptiMEM medium buffered with 25 mM HEPES (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich), α-ZC3HAV1 (GTX120134 ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich) or with isotype control (12-370 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured with lentiviral solution for 8-12 h in the presence of 8 μg/mL Polybrene (Sigma- Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: Artc1-KO and Artc1-WT MEFs were incubated on 96-well plates (1×104 cells/well) for 24 h before exposure to H2O2 (0-100 µM, Sigma). Necrosis inhibitor IM-54 (0-10 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... 743 and 732 cells were then infected with lentiviruses for 24 h using Polybrene (final concentration of 10 μg/mL; Sigma) in high-glucose DMEM supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cancer Biology 2023Quote: ... 725 and 731 cells were then infected with lentiviruses for 24 h using Polybrene (final concentration of 10 μg/mL; Sigma) in high-glucose DMEM supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Developmental Biology 2023Quote: ... was removed by incubating the embryos in pronase (0.5% w/v Proteinase K in H-KSOMaa supplemented with 0.5% PVP-40 (Sigma-Aldrich, P0930)) covered with mineral oil for 2-3 minutes ...
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bioRxiv - Genetics 2022Quote: ... and PR110 (h+ leu1-32 ura4-D18) were used for three successive rounds of mutagenesis with ethylmethane sulfonate mutagenesis (EMS from Sigma) based on [35] ...
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bioRxiv - Cell Biology 2023Quote: ... filtered and applied onto the desired HeLa cells for 24-48 h in the presence 8 mg/ml polybrene (Sigma). Cells were then recovered and expanded in growing DMEM for 5-7 days prior to sorting by FACS to match protein expression levels where possible.
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bioRxiv - Neuroscience 2023Quote: ... permeabilised by washing in 0.1% Triton-X in PBS (PBS-T) for 15 min and incubated for 2 h in a blocking buffer of 2% bovine serum albumin (Sigma) and 3% normal donkey serum (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at least 12 larvae were pooled together per sample in 1.5 ml tube and incubated for 1 h at room temperature in 200 µl of the extraction buffer: 4% IGEPAL (Millipore, Sigma) in 100% isopropanol ...
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bioRxiv - Microbiology 2023Quote: ... After blocking with 5% BSA the membrane was incubated for 1 h with 50 µg of purified PLG (Sigma-Aldrich) diluted in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 h the medium was replaced with a selection medium supplemented with 2 μg/ml Puromycin (Sigma. Cat# P9620). The cells that survived and proliferated in 2 μg/ml Puromycin were selected ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Primary neurons were infected for 24 h then protein was extracted by sonication in RIPA buffer supplemented with Protease Inhibitor Cocktail (Sigma) and Phosphatase Inhibitor Cocktail 2 (Sigma) ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... supernatant containing replication-incompetent lentiviruses was filtered and applied for 24 h on the target cells in a medium containing polybrene 8 μg/mL (Sigma) (78 ...
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bioRxiv - Physiology 2024Quote: ... Cells were serum starved (0.5% FBS) for 1 h and treated with or without insulin (100 nM; Sigma, St. Louis) for 6 h at 37°C for proteomics experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Expanded colonies were tested by inducing ER stress with 200 nM thapsigargin for 6 h (Sigma-Aldrich, Burlington, MA, USA) using confocal microscopy (Zeiss LSM800 controlled by the ZEN 2.3 software ...
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bioRxiv - Immunology 2024Quote: ... The concentrated retrovirus was added to 24 h-activated T cells with 8 ng/mL polybrene (#TR-1003, Sigma-Aldrich), 200 U/mL IL-2 (#212-12 ...
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bioRxiv - Developmental Biology 2024Quote: The zona pellucida of 2-cell embryos was removed with H-KSOM medium containing 0.1% protease (Sigma, St. Louis, MO). Individual blastomeres from the zona-free embryos were obtained by repeatedly pipetting in Ca2+-free and Ma2+-free H-KOSM medium ...
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bioRxiv - Cell Biology 2024Quote: ... S2 cells lysates containing 2 mg of total proteins were precleared for 1.5 h under agitation at 4 °C with 15 µl of sepharose CL-4B beads (Sigma-Adrich). Supernatants were then incubated with 15 µl of GFP-Trap beads (ChromoTek ...
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bioRxiv - Genomics 2024Quote: ... and incubating them for 24 h with 50 µl of the regarding lentiviral supernatant and 7.5 μg/mL polybrene (Sigma-Aldrich). To select for successfully transduced HFF ...
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bioRxiv - Biophysics 2024Quote: ... 80 mg of cleaned wool (R. H. Lindsay Wool Company) was soaked into 4 ml of salt solutions with 0.1 M DTT (Sigma-Aldrich) in quartz cuvettes ...