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Citations for Millipore Sigma :
3951 - 4000 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... virgin female flies were collected within 6 h of eclosion and reared on standard medium supplemented with 1.5 mM methoprene (Sigma-Aldrich, St Louis ...
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bioRxiv - Immunology 2022Quote: ... TZM-bl cells were added to each well and incubated for 48 h at 37°C in presence with 12.5 μg/mL DEAE-Dextran (Sigma-Aldrich). Prior to data acquisition ...
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bioRxiv - Microbiology 2022Quote: ... The cells were further incubated at 37°C for 72 h before being stained with 1% crystal violet (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Pathology 2020Quote: ... after 12 h fasting by a single intraperitoneal (i.p.) injection of streptozotocin (STZ) (Sigma Chemical Co., St, Louis, MO, USA) at a dosage of 60mg/Kg of body weight (BW ...
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bioRxiv - Synthetic Biology 2020Quote: ... Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm, Millipore Sigma, Cat. No. SLHA033SS).
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated for 1 h at room temperature or overnight at 4°C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Microbiology 2020Quote: ... 20 μL of the elution samples were incubated for 1 h at room temperature with 1 μL of thrombin (thrombin from bovine plasma, Sigma). Samples were analyzed via SDS-PAGE and immunoblotting as indicated in the figure legends.
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bioRxiv - Pathology 2021Quote: ... Stage V-VI oocytes were selected after enzymatic defolliculation (2 h of gentle shaking in a collagenase-containing solution (1A, Sigma) at 16°C) ...
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bioRxiv - Neuroscience 2020Quote: ... 3% Triton-X in PBS) for 1 h before incubation with primary antibodies (1:1000 anti-tyrosine hydroxylase, #AB152, Millipore UK ...
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bioRxiv - Molecular Biology 2021Quote: ... Zygotes were harvested from oviducts at 28 h after hCG injection and then cultured in KSOM Mouse Embryo Media (Millipore) at 37°C with 5% CO2 ...
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bioRxiv - Physiology 2021Quote: ... The effects of different ionophores on glucosinolate transport was studied by incubating the PaGTR1-expressing oocytes in Kulori buffer at pH 6.0 containing either 20 μM carbonyl cyanide m-chlorophenyl hydrazone (H+ionophore, Sigma-Aldrich), 20 μM nigericin (K+/H+ exchanger ...
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bioRxiv - Biochemistry 2021Quote: ... coli B834(DE3) carrying pET28b(+)-phm7 was cultured at 18 °C for 96 h using the Overnight Express Autoinduction System 2 (Novagen). Cells expressing the DS were harvested by centrifugation and frozen at –80 °C until use.
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bioRxiv - Microbiology 2020Quote: ... 6 h post transfection cells were fixed in 4% PFA and permeabilised with 0.1% (v/v) Triton X-100 (Sigma-Aldrich)in PBS for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Non-infected and Lm-infected HUVEC were incubated for 1 h with primary antibody rabbit anti-STAB-1 (1:100, Millipore), diluted in 0.2% saponin (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... were infected with lentiviral pLKO.1-puro vectors expressing specific shRNA sequences for 24 h in the presence of polybrene (8μg/ml; Sigma-Aldrich). After further 24 hrs ...
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bioRxiv - Microbiology 2020Quote: ... Lungs from intranasally immunized mice were harvested and digested for 1 h at 37°C in digestion buffer consisting of RPMI media supplemented with (167 μg/ml) of Liberase DH (Sigma) and (100 μg/ml ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the following primary antibodies for 1 h at room temperature in Abdil: mouse anti-human lamin A/C (1:200; Millipore), rabbit anti-lamin A/C (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were discarded ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were plated (2×107 cells per 150 mm plate) and grown for 24 h before transfection with Polyethylenimine (PEI, Sigma). After 72 h ...
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bioRxiv - Molecular Biology 2021Quote: ... After a release for 8 h in fresh media they were incubated with 50 ng/mL nocodazole (Sigma-Aldrich, M1404) for 16 h ...
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bioRxiv - Biochemistry 2021Quote: 100.106 haploid HAP1 cells at 60 % confluency were treated for 72 h with 0.3 mg/mL ethyl methanesulfonate (EMS, Sigma-Aldrich) directly added to the cell medium ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Obtained complemented strains were grown at 37°C for 16 h in BHI supplemented or not with 1 mM of IPTG (Sigma) until stationary phase was reached ...
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bioRxiv - Bioengineering 2021Quote: The collected BMOs were fixed with 2 % paraformaldehyde (PFA, ThermoFischer Scientific, 15434389) for 1 h and permeabilized with 0.3 % Triton-X-100 (Sigma, T8787) in PBS for 3 h at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... Metaphase II-arrested oocytes tightly packed with cumulus cells were collected from the oviductal ampulla 14 h after hCG injection and placed in a 100-μl drop of human tubal fluid (HTF; Millipore) medium covered with mineral oil ...
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bioRxiv - Microbiology 2020Quote: ... The PVDF membrane was blocked for 1 h in PBS supplemented with 0.1 % Tween-80 from Sigma Aldrich (‘PBS-Tween’) and 4 % of milk powder ...
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bioRxiv - Microbiology 2020Quote: ... lysates were pre-cleared for 1 hour (h) with mouse immunoglobulin G (IgG) agarose or rabbit immunoglobulin G (IgG) agarose (Sigma), dependent on the species of antibody used in IP ...
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bioRxiv - Immunology 2021Quote: ... or 2 μM NH-3 in the presence of 10 nM T3 for 24 h before the addition of 3 μL of a fluorescent latex bead suspension (L0280, Sigma) in complete DMEM in a ~100:1 bead-to-cell ratio for 2 h before the end of the experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3+ T and Jurkat cells were cultured with lentivirus solution for 24 h in the presence of 8 μg/mL Polybrene (Sigma).
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Immunology 2021Quote: The S-variant and RBD coupled bead sets were incubated with sera from immunized mice at 4 dilutions (1:100; 1:1000; 1:10000; 1:100000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) and analyzed on a Luminex analyzer (Luminex / R&D Systems ...
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bioRxiv - Immunology 2020Quote: Wild-type zebrafish embryos were dechorionated at 24 h post fertilization (hpf) using digestion with 1 mg/ml pronase (Sigma) in E3 medium (83 μM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... in 1X PBS with 0.1% of Tween 20 (PBS-T) for 1 h and probed with a 1:3,000 dilution of α-FLAG-HRP antiserum (Sigma), 1:1,000 dilution of α-AzuC antiserum (New England Peptide) ...
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bioRxiv - Immunology 2021Quote: ... cells were primed with LPS (1 µg/mL) for 4 h and treated with UCN-01 (10 µM, Sigma-Aldrich), nigericin (10 µM ...
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bioRxiv - Genetics 2020Quote: ... the cells were incubated in serum-free MEM for 16 h prior to treatment with S1P (Sigma-Aldrich, MO, USA) and FAM19A5 ...
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bioRxiv - Neuroscience 2022Quote: 100,000 glioma cells were plated onto circular glass coverslips (Electron Microscopy Services) pre-treated for 1 h at 37 °C with poly-L-lysine (Sigma) and then 1 h at 37 °C with 10 µg/ml natural mouse laminin (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... and subcultured for 72 h in NK MACS medium supplemented with 5% human serum male AB plasma (#H4522, Sigma-Aldrich) and human IL-2 IS (200 IU/ml ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10; anti-FLAG M2, 1:1,000 dilution, Sigma, F1804).
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bioRxiv - Bioengineering 2022Quote: ... the cells were kept at dim light (2-3 μE) for 16 h and plated on fresh TAP plates containing 10 μg/mL Paramomycin (Sigma). Plates were incubated for 10 d at 30-50 μE constant light ...
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bioRxiv - Biochemistry 2022Quote: ... and serially diluted in DMEM media and incubated in 24-well plates with a semi-confluent culture of Vero cells (for ZKV samples) for 1 h at 37°C and covered with DMEM 2% fetal bovine serum + 0.8% of methylcellulose (Sigma, M0512) overlay for 4 days at 37°C and 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2022Quote: Cells were incubated with lentiviral supernatant for 24 h in the presence of 8 μg/μl of polybrene (Sigma-Aldrich) at a multiplicity of infection >5 unless otherwise stated.
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bioRxiv - Developmental Biology 2022Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA, Sigma) for 45 min in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... sperm recovered from 12-15 week old male MF1 mice was capacitated for 2 h in a 500-μl drop of HTF medium (Sigma), layered by mineral oil (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were incubated in a rotating incubator for 1 h at 4°C with bead-conjugated FLAG (Sigma; A2220), bead-conjugated HA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...