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Citations for Millipore Sigma :
3901 - 3950 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Whole cellular protein was extracted with RIPA buffer containing 1 mM Na3VO4 (Sigma-Aldrich) and protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected by Western blot using HRP-conjugated mouse anti-FLAG (Sigma, A8592) and rabbit polyclonal anti-GFP (Chen et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Protein-containing fractions were pooled and concentrated using Amicon centrifugal units (MWCO 30,000, Millipore), centrifuged (17,000xg ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were then resolved with SDS-PAGE and blotted to PVDF transfer membrane (Millipore). The presence of the proteins of interest was tested by immunodetection using rat anti-HA-peroxidase (3F10 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (pore size, 0.45μm; Millipore Sigma, USA) and blocked with 5% nonfat dry milk in Tris-buffered saline (20mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... Protein was concentrated using a Amicon Ultra 15mL 3K NMWL centrifugal filter (Millipore Sigma).
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bioRxiv - Physiology 2019Quote: Proteins were extracted from tissues using Ripa buffer and protease inhibitor cocktail (Sigma-Aldrich). Tissues homogenates were centrifuged twice for 20 min at 12700 rpm and supernatants were quantified with BCA pierce kit (ThermoScientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Captured protein complexes were eluted with 30μl of 1N Ammonium Hydroxide (#320145, Sigma-Aldrich), 5mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated in a 10k MWCO Amicon-Ultra centrifugal filter (Millipore Sigma) and loaded onto a Superdex 200 HiLoad 16/60 gel filtration column equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... We induce protein expression by adding 1 mM isopropyl β-d-1-thiogalactopyranoside (Sigma) when the cell density reaches an ODλ=600nm of 0.6 ~ 0.7 ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2021Quote: ... the specific proteins were visualized with a chemiluminescence HRP Substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins for size-exclusion column calibration were purchased from Sigma-Aldrich (St. Louis, MO). 5-aminolevulinic acid was purchased from Carbosynth (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were concentrated by centrifugation with a 10 kDa cut-off membrane (Merck Millipore) to a volume of 300 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were transferred to PVDF (polyvinylidene difluoride) membranes (0.2 μm pore size, Millipore) by immersion transfer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Protein lysates were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Millipore). After the membranes were blocked using 5% skimmed milk (Megmilk Snow Brand ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was concentrated to 5 mg·ml−1 in an Amicon ultracentrifugation device (Millipore) with 10 kDa cutoff and directly used for further studies or supplemented with 40% glycerol ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted with Wash Buffer containing 0.2 mg/ml 3xFLAG peptide (Sigma). CSB proteins were further purified by gel filtration (Superdex 200 Increase ...
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bioRxiv - Neuroscience 2020Quote: ... A pre-clear step was performed by adding protein A-agarose beads (Merck Millipore) to the lysates ...
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bioRxiv - Neuroscience 2020Quote: Mouse anti-Myelin Basic Protein (MBP-SMI-99, Millipore, reference NE1019, 1/1000 dilution), rat anti-Myelin Basic Protein (BIO-RAD ...
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bioRxiv - Biochemistry 2020Quote: Solutions of intact and deglycosylated proteins were passed through the ZipTip C18 microcolumns (Millipore), washed and eluted according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... the target proteins were transferred onto PVDF membranes (Millipore Durapore, 0.45 μm pore size). The membranes were washed ...
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bioRxiv - Neuroscience 2019Quote: ... The antibody was captured using 30 μl of 50% Protein G Sepharose beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Microbiology 2019Quote: ... and the soluble proteins were separated from the OMVs using an iodixanol (Optiprep, Sigma) density gradient ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies used were rabbit anti-prosurfactant protein C (SPC) (Millipore, AB3786, 1:500) and goat anti-IL-33 (R&D ...
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bioRxiv - Microbiology 2019Quote: ... 10 mg of total proteins were mixed with Magnetic anti-FLAG beads (Sigma, USA) and incubated over-night with end-over-end rotation at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2019Quote: ... Protein was concentrated using a centrifugal concentrator with an MWCO below 15 kDa (Millipore). The concentrated protein was sub-aliquoted ...
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bioRxiv - Bioengineering 2020Quote: ... Proteins were then reduced with 10 mM DTT/40 mM NH4HCO3 (Sigma-Aldrich Co.) at 56°C for 20 min ...
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bioRxiv - Genetics 2020Quote: The GST-Clr4 fusion protein was allowed to bind to Glutathione-agarose beads (Sigma) in MTPBS buffer (100mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... The purified VxrB protein was concentrated by centrifugal filter devices (Millipore, 3,000 MW CO) before injection onto High load TM (16/60 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mg of each sample was precleared with EZview protein G beads (Sigma-Aldrich) for 0.5 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: Nuclear extracts were pre-cleared by incubation with 40 μl Protein A dynabeads (Sigma) for one hour at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Soluble protein was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.). Absorbance of each reaction was measured in a spectrophotometer (Spectra Max M2 ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The collected protein fractions were pooled and concentrated using an Amicon ultrafiltration cell (Millipore) and dialyzed into PBS (10 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2021Quote: Proteins resolved through SDS-PAGE gels were transferred to Immobilon-P PVDF membranes (Millipore) and blocked with 3% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein expression for all constructs was conducted using Rosetta II pLac(I) cells (Millipore) grown to high density at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were ultrafiltrated using 10 K pore size Amicon Ultra filter units (Millipore) and step-wise dialyzed against 5 mM Pipes-Na pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was induced with 0.25 mM IPTG in E.coli Rosetta BL21(DE3) (Novagen) in the presence of 50 µM ZnCl2 at 37° C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 55 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 μg of purified protein was mixed with 2 mM of acetyl phosphate (Sigma) in 100 μl of phosphorylation buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... The secreted S proteins were purified by anti-FLAG M2 affinity resin (Sigma Aldrich). After loading two times ...
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bioRxiv - Cell Biology 2021Quote: ... we inhibited global protein synthesis with cycloheximide (CHX, 100 μg/mL, Sigma Aldrich # C4859).
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bioRxiv - Molecular Biology 2021Quote: ... Total proteins were extracted from transfected 293T cells using RIPA lysis buffer (Millipore Sigma) at 48 hpt ...