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Citations for Millipore Sigma :
351 - 400 of 4313 citations for Recombinant Human APP His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Genetics 2023Quote: Human mCherry-tagged CTNS mRNA solutions were prepared in RNAse free water (500ng/µl) with 0.05% PhenolRed (Sigma, cat.no.P0290). All mRNA injections were performed at the one-cell stage using a FemtoJet microinjector (Thermofisher ...
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bioRxiv - Plant Biology 2022Quote: ... GST-OsIDD12 and GST-OsIDD13 fusion proteins were expressed in Escherichia coli BL21(DE3) Rosetta strain (Novagen, USA). Frozen cells were extracted with 100 μl of extraction buffer containing 50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-Rpb4 and GST-HRP (A3740, SIGMA).
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... anti-GST rabbit 1/100,000 (G7781, Sigma), anti-MBP mouse 1/80,000 (E8032S ...
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bioRxiv - Cell Biology 2021Quote: a-GST (mouse monoclonal IgG1, Sigma #SAB4200237)
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bioRxiv - Plant Biology 2020Quote: ... 1:10000 for anti-GST (SIGMA, USA), 1:5000 for anti-HA (SIGMA ...
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bioRxiv - Biochemistry 2021Quote: ... in place of the GST gene (Novagen).
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bioRxiv - Cell Biology 2019Quote: ... and GST (1:10,000, Sigma A-7340). Appropriate horseradish peroxidase-labeled secondary antibodies (Jackson Immunoresearch ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-GST antibody (Sigma Aldrich, 06-332) was added diluted 1:1,000 in PBS-T with 3% BSA and incubated with gentle agitation in RT for 1 hr ...
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bioRxiv - Plant Biology 2023Quote: ... 1:5,000 mouse anti-GST (Sigma SAB4200237); 1:2,000 rabbit anti-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Biochemistry 2024Quote: ... a primary anti-GST (Sigma, 1:3,000) was used to detect bound PI(4,5)P2-grip protein.
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... transferred to nitrocellulose membrane and probed with anti-APP-CTF antibody (Sigma, cat# A8717).
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bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted fractions from each pull down (Ni-NTA and GST-affinity) were probed with anti-GST (1:3000) antibody (Sigma) and anti-His (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: 0.5 μM RABL3 and 0.05 μM GST or GST-RAB11 were mixed with Glutathione-Sepharose 4B in the presence of 10 μM GTPγS (Sigma Aldrich) or 10 μM GDP (Sigma Aldrich ...
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bioRxiv - Plant Biology 2021Quote: The coding sequence of Hd3a and RFT1 were cloned into the pCold-GST vector42 and produced as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 Rosetta (DE3) (Novagen). Cells were grown in LB medium or minimal medium containing 0.5 g l™1 of 15N-ammonium chloride ...
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bioRxiv - Cell Biology 2021Quote: ... polyclonal anti-GST rabbit IgG (Millipore-Sigma, G7781), StarBright® B700 labeled goat anti-mouse IgG secondary antibody (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... polyclonal anti-GST rabbit IgG (Millipore-Sigma, G7781), StarBright® B700 labeled goat anti-mouse IgG secondary antibody (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GST tag (G1160, Sigma 1:2000), mouse anti-His tag (HIS.H8 / EH158 ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag was removed by thrombin (Millipore) cleavage and the cleaved product exchanged into NMR buffer (50 mM Na phosphate at pH 6.7 ...
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bioRxiv - Biochemistry 2021Quote: ... GST (G7781) and Flag (F3165) from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-GST (Sigma G7781, 1:3,000).
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bioRxiv - Plant Biology 2022Quote: ... detected via anti-GST antibody (Sigma Aldrich, A7340), served as a positive control (not shown).
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bioRxiv - Pathology 2023Quote: ... and α-GST (1:10, 000, Sigma-Aldrich). Secondary α-rabbit ...
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bioRxiv - Genetics 2023Quote: ... Primary rabbit anti-GST polyclonal antibody (Sigma, G7781) (1:160,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:4000 rabbit anti-GST antibody (G7781, Sigma) and mouse anti-His (1:2500 ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-GST antibodies (Sigma-Aldrich, Darmstadt, Germany).
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bioRxiv - Cell Biology 2023Quote: ... followed by anti-GST (1:500, Sigma, SAB4200692) and secondary staining anti-Mouse-DyLight405 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... anti-glutathione-S-transferase (GST) (G7781; Sigma-Aldrich), anti-InlC (15) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...