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Citations for Millipore Sigma :
251 - 300 of 4313 citations for Recombinant Human APP His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... GST-assay (Sigma CS0410) was likewise adjusted from a 96- to a 384-well plate using our own reagents ...
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bioRxiv - Physiology 2019Quote: ... GST-assay (Sigma CS0410) was adjusted from 96-to 384-well plate ...
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bioRxiv - Microbiology 2021Quote: ... anti-GST (Sigma, USA), and anti-BSA (Sangon Biotech ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibody anti-APP (22C11 mouse 1:500, Millipore MAB348) was incubated overnight at 4 °C and anti-mouse-HRP-coupled secondary antibody (1:3000 ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies against C-terminal APP (Sigma A8717, 1:10,000), β-actin (Sigma A5441 ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse anti APP A4 clone 22C11 (Millipore MAB348, 1:1,000), Mouse anti APP clone 6E10 (Biolegend 803001 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-amyloid precursor protein (APP, clone 22C11, Millipore), mouse monoclonal anti-α-Synuclein (Syn-1 ...
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bioRxiv - Neuroscience 2022Quote: Primary antibodies against C-terminal APP (Sigma A8717, 1:10,000), β-actin (Sigma A5441 ...
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bioRxiv - Neuroscience 2024Quote: ... The antibodies used were mouse APP (Sigma: MAB34; 1:1000) and rabbit TUJ1 (Sigma ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... GST- hAgap1_PH_L and GST-hAgap1_PH_S were transformed into Rosetta 2(DE3)pLysS (Novagen) and proteins were expressed in Luria Broth based auto-induction medium including trace elements (Formedium ...
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bioRxiv - Plant Biology 2024Quote: ... or anti-GST (Mouse anti-GST monoclonal antibody, Sigma SAB4200692, 1:5000 dilution), respectively ...
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bioRxiv - Plant Biology 2023Quote: 6xHistidine-Glutathione-S-transferase (GST) tagged proteins of the predicted intracellular region of SOBIR1 (termed the KD) was cloned into pCDF-Duet vector (Novagen, EMD Chemicals, San Diego, CA) and expressed in E ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology ...
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bioRxiv - Biophysics 2020Quote: The recombinant human Mdm2 (1-125 aa) were cloned into pET11a vector (Novagen) and expressed in E ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng/ml cholera toxin and 10 μg/ml recombinant human insulin (Sigma). MCF10A I-PpoI cells and MCF10A AsiSI cells were previously described (Harding et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... JSH23 (Cat# 481408) and Recombinant human IGF-1 (Cat# GF138) purchased from Millipore; JQ1 (Cat# M2167 ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich, St. Louis, MO), 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10 ng/ml of human rIGF1 (recombinant Insulin growth factor-1) (Sigma, USA), 10 ng/ml amphotericin B ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days ...
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of sample or standard (Recombinant human IFN-α, IFNA: Millipore, IF007) were added ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng per well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Neuroscience 2021Quote: ... GST (Sigma, 27-4577-01), Flag (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-GST HRP conjugate (SIGMA) were used in a 1:2000 dilution.
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bioRxiv - Cell Biology 2020Quote: ... anti-GST (G7781, Sigma Aldrich); anti-His (ab18184 ...
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bioRxiv - Biochemistry 2021Quote: ... 1:2500 anti-GST (Sigma) and 1:2000 anti-Myc (ab9106 ...
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bioRxiv - Cell Biology 2023Quote: ... GST (Millipore Sigma #05-782), and T7 (Millipore Sigma #69522) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Alzheimer Precursor Protein (APP) 22C11 (1:100, MAB348, Millipore), and mouse anti-β-Tub III/Tuj 1 (1:4000 ...
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bioRxiv - Neuroscience 2020Quote: ... or a rabbit anti-APP antibody (Sigma, USA, #A8717, 1:4,000) or an anti-Cystatin B rat monoclonal antibody (Novus Biologicals ...
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bioRxiv - Neuroscience 2020Quote: ... or mouse anti-FlagM2 and rabbit anti-APP-CTF (Sigma-Aldrich) at 4 ng/μL and then pulled-down for 2 h using protein G-coupled dynabeads (50 μL ...
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bioRxiv - Neuroscience 2023Quote: ... and amyloid precursor protein (APP) (A8717, Sigma-Aldrich, St. Louis, MO) or monoclonal antibody against β-actin (A2228 ...