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Citations for Millipore Sigma :
451 - 500 of 4313 citations for Recombinant Human APP His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... and 50 μg of total protein were loaded on 12% acrylamide gel for determination of APP (Sigma), Amyloid-β (Aβ ...
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bioRxiv - Neuroscience 2023Quote: ... anti-rabbit TRITC tagged (1:200, Sigma) and anti-mouse FITC tagged (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-GST primary antibodies (Sigma□Aldrich, Saint Louis, MO) and secondary goat antimouse IgG:IRDye800CW (Li-Cor).
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bioRxiv - Cell Biology 2019Quote: ... Western blot analysis was performed with α-GST (Sigma G7781), α-His (Sigma H1029) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of monoclonal mouse-anti-GST antibodies (Sigma-Aldrich), monoclonal anti-His6 antibodies (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... GST tag was then cleaved off using preScission protease (Sigma) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... and analyzed by immunoblotting using either anti-GST antibody (Sigma) or anti-MBP antibody (NEB).
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bioRxiv - Biochemistry 2021Quote: ... Soluble lysates were incubated with GST-Bind resin (EMD Millipore) for 30 min at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... H9 cells were cultured with 6μM Chir99021 (GST inhibitor [Millipore]) in B27-medium (RPMI 1640 medium [Invitrogen] supplemented with 1× B27 minus insulin [50× ...
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bioRxiv - Biochemistry 2022Quote: ... GST proteins were eluted with 10 mM reduced glutathione (Sigma) in 50 mM Tris pH 8.0.
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bioRxiv - Cell Biology 2022Quote: ... Anti-GST-HRP (RPN1236, 1:10000) was from Sigma Aldrich. Anti-phosphotyrosine antibody (4G10 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with anti-GST mAb (1:2000, Sigma) and anti-mouse IgG-HRP (1:2000 ...
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bioRxiv - Biochemistry 2023Quote: ... GST-Erk2 at 20 μg/mL (Sigma-Aldrich, ref. E1283), GST-Cdk1/CyclinA2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... GST was removed with the Thrombin CleanCleaveTM Kit (Sigma-Aldrich) following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... GST protein was purified using glutathione Sepharose 4B beads (Sigma) by rotating overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and input TRF1 mutants were revealed with anti-His (anti 6-His Rabbit Pab Sigma Aldrich). For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Cell Biology 2023Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Biophysics 2021Quote: ... or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Microbiology 2019Quote: ... or anti-His (1:1000 Sigma) overnight at 4 °C and anti-mouse secondary (1:5000) ...
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bioRxiv - Microbiology 2021Quote: ... α-His (Sigma, cat# H1029, 1: 3,000), α-ICDH (1 ...
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bioRxiv - Microbiology 2020Quote: ... α-His(Sigma, cat# H1029, 1: 10,000), α-HA (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... anti- HIS from Novagen (70796-3), anti-TGN38 from BD Transduction (610898) ...
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bioRxiv - Biochemistry 2019Quote: His-select Cobalt affinity beads (Sigma) were equilibrated in binding buffer (40 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-His (Sigma-Aldrich, H1029, RRID:AB_260015), anti-Strep (IBA GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-HIS (Sigma H1029 1:2,500) or anti-MBP (New England Biolabs E8032 ...
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bioRxiv - Neuroscience 2023Quote: ... His•Tag® (Millipore, 70796-3), anti-APP C-Terminal Fragment (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Biochemistry 2020Quote: ... and mouse monoclonal anti-APP (1:500, 22C11; Cat#Mab348, RRID: AB_94882, Merck Millipore/Sigma, Burlington, MA, USA) antibodies were purchased from the indicated suppliers ...
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bioRxiv - Biochemistry 2020Quote: ... and mouse monoclonal anti-APP (1:500, 22C11; Cat#Mab348, RRID: AB_94882, Merck Millipore/Sigma, Burlington, MA, USA) antibodies were purchased from the indicated suppliers ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated at 4 °C overnight with primary antibodies against full-length APP (1:1,000, Millipore), APP-CTFs (1:1,000 ...
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bioRxiv - Pathology 2020Quote: ... Slides were incubated overnight at 4°C using either an anti-mouse APP (22C11) (Millipore, MAB348, 1:80,000), an anti-mouse GFAP (SMI-22 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...