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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for PGFM ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... at 37°C and 5% CO2 in RPMI-1640 (Biological Industries, cat.no. 01-101-1A) supplemented with 5% fetal bovine serum (FBS, Sigma Aldrich, cat.no. F6178), 2 mM L-glutamine (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary guide oligonucleotides (forward: 5’-CACCGCAGGATTGAAGACCTTAACA-3’; reverse: 5’-AAACTGTTAAGCTCTTCAATCCTGC-3’) were custom synthesized separately by Sigma-Aldrich (St. Louis, MO, USA), annealed ...
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bioRxiv - Cell Biology 2021Quote: ... The suspension was then centrifuged and the resulting pellet was washed with 5 ml of 5% FBS (Sigma-Aldrich, Oakville, ON, Canada) in 1× DPBS (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... Immunostained cells were washed 3x for 5 min in PBST and 3x for 5 min in PBS before being incubated with Hoechst 33342 (Sigma-Aldrich, B2261) for 15 min at 25 °C ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5 mM DTT) and concentrated to ∼ 5 mg/mL (Amicon Ultra 4 mL Centrifuge filters, 30 KDa NMWL devices, Millipore Cat# UFC805024). Last ...
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bioRxiv - Developmental Biology 2021Quote: ... 60 µl elution buffer (2 M urea, 50 mM Tris-HCl pH 7.5 and 5 μg/mL Trypsin (SIGMA; T6567-5×20UG)) (twice as the bead volume ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed in 1X PBS and co-cultured at a ratio of 5 parasites per macrophage (MOI 5:1) in RPMI 1640 medium without L-arginine (R1780, Sigma-Aldrich, USA) supplemented with 2% of inactivated FBS (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... 5 mL of whole human blood was layered onto 5 mL of Histopaque-1077 Hybri-MaxTM (Sigma Life Science, H8889-100 mL) in a column ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... cells were permeabilized with 0.1% Triton X in PBS at room temperature on agitator for 5 min and then blocked with 5% bovine serum albumin (Sigma-Aldrich, A9647) in PBS for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using puromycin (5 µg/ml Sigma-Aldrich, Poland). Selected cells were plated on insert supports and further cultured in ALI in the presence of puromycin (1 µg/ml).
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bioRxiv - Microbiology 2020Quote: ... 49 with 1.5% weight vol−1 agar supplemented with 2 mg mL−1 5-Fluoroorotic acid (USBiological, USA) and 5 µg mL−1 uracil (Sigma–Aldrich USA) in order to select for plasmid excision ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were then washed 3 times in 0.5% PBSTx for 5 minutes per time and incubated in Hoechst 33258 (use: 1 in 1000; stock: 20mg/ml; Sigma-Aldrich; B2883) for 30-60 minutes.
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bioRxiv - Neuroscience 2022Quote: ... We combined this immunohistochemistry with the one for serotonin (5-HT) using the rabbit IgG anti-5-HT (1/500, Sigma-Aldrich, 49545) and the goat anti-rabbit Fluor Alexa 568 (1/500 ...
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bioRxiv - Cell Biology 2024Quote: ... PVDF membranes with proteins were washed with 0.1% Tween-20 in Tris-buffered saline (TBST) for 5 min and blocked with TBST + 5% bovine serum albumin (BSA; Sigma Aldrich #A3059) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... each antibody was incubated with HIV-1 pseudovirus for 1 hour at 37°C at concentrations from 5 µg/mL to 5×10−5 µg/mL using 10-fold serial dilution in complete DMEM with 15 µg/mL DEAE-dextran (Sigma-Aldrich, D9885). Culture media was aspirated from the TZM-bl cells and replaced with antibody-virus mixture ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... inhibitors were freshly diluted serially from stocks such that the same percentage (0.1%) of DMSO (Goldbio 67-68-5, Millipore-Sigma 67-68-5) was added to 1X E3 in glass vials (Fisherbrand 03-339-22B) ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were incubated for 6 h at 37 °C under a humidified atmosphere with 5% CO2 in the presence of 5 μM carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (Sigma-Aldrich, USA) to induce mitophagy ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli ST18 strain was grown at 37°C in media supplemented with 50 µg/ml of 5-aminolevulonic acid (5-ALA, Sigma-Aldrich, USA), with or without shaking (120 rpm shaking rate) ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μl papain inhibitor solution containing 5 mg/ml BSA (Carl Roth, 8076.4) and 5 mg/ml Trypsin inhibitor (Sigma-Aldrich/Roche, 10109878001) in PBS was added ...
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bioRxiv - Microbiology 2023Quote: ... incubated with lysozyme reaction buffer (0.8X PBS, 50 mM glucose, 5 mM EDTA, 0.1% Triton X-100, 5 mg/mL lysozyme [Sigma-Aldrich catalog number L6876]) for 20 min at 37°C to permeabilize bacteria ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides with tissue sections were rinsed three times with PBS and incubated for 5 minutes in 5% saponin (Sigma-Aldrich, #S7900) solutions following three washes of PBS ...
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bioRxiv - Developmental Biology 2024Quote: Embryos collected from natural spawning were injected at the vegetal pole at 5 mpf with 1nl of 1:5 fluorescent yellow-green carboxylate-modified polystyrene latex beads (L4530, Sigma-Aldrich®) diluted in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... 60 mM sucrose [S7903, Sigma], 5 mM KH2PO4 [P5379, Sigma], 5 mM MOPS [M1254, Sigma], 1 mM EGTA [E4378, Sigma], pH 7.2) and 3 mg of proteinase (P8038 ...
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bioRxiv - Developmental Biology 2024Quote: ... Tg(actab2:loxP-BFP-STOP-loxP-dsRed)sd27 fish were treated overnight at 5 dpf with 5 μM (Z)−4-Hydroxytamoxifen (4-OHT) (Sigma-Aldrich H7904) in EM ...
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bioRxiv - Developmental Biology 2024Quote: ... Differentiation day 18 control and proximal-biased organoids were incubated overnight (18 hrs) at 37°C/5% CO2 in TeSR-E6 supplemented with 5 µM cisplatin (Millipore Sigma, 232120). Organoids recovered between days 19 and 20 ...
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bioRxiv - Plant Biology 2019Quote: ... and 0.5 mg/mL 5(6)-Carboxyfluorescein diacetate (Sigma) was applied to the cut using a P2 pipette ...
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bioRxiv - Biophysics 2021Quote: ... 5 µM Thioflavin T (ThT, Fluka, Sigma-Aldrich, UK) and different concentrations of NaCl (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... or 5 mM of Zn(NO3)2 (Sigma-Aldrich) and Mg(NO3)2 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... or Adenosine 5’-diphosphate sodium salt (ADP; Sigma-Aldrich) was added so the final concentration for each substrate was 20 mM.
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bioRxiv - Cancer Biology 2021Quote: ... 5 mg/mL BSA: Bovine Serum Albumin (Sigma, A3059), 1x L-Glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... Decitabine (5-aza-deoxycytidine) was from SIGMA-ALDRICH (A3656). For RT-qPCR analysis ...
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bioRxiv - Biophysics 2021Quote: ... Adenosine 5’-triphosphate disodium salt hydrate (ATP; Sigma-Aldrich), D-(+)-Glucose (Glu ...
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bioRxiv - Bioengineering 2021Quote: ... distal femurs were stained with 5’-DTAF (Sigma-Aldrich) to label the cartilage ECM ...
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bioRxiv - Biophysics 2021Quote: ... and 5(6)-carboxyfluorescein were purchased from Sigma-Aldrich. Methanol (LC/MS grade ...
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bioRxiv - Neuroscience 2021Quote: ... When carbamoycholine chloride (CBC, 5-20 µM, Sigma Aldrich), atropine (10 µM ...
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bioRxiv - Developmental Biology 2021Quote: ... 5% (v/v) Donkey serum (Merck Millipore, S30-100ML) for more than 1h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Media supplemented with 5 μg/mL puromycin (Sigma Aldrich) or was changed 48h after transduction to select lentiCRISPRv2 transduced cells ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 µg/mL arginine (Sigma-Aldrich A5131-100G), while heavy media included 30 µg/mL 13C6 lysine (CK Isotopes CLM-2247-H) ...
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bioRxiv - Cell Biology 2020Quote: ... 5% foetal bovine serum and 1% penicillin/streptomycin (Sigma) at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... adipocytes were transfected with 5 nM siRNA (Sigma-Aldrich) using 5 μl/ml Lipofectamine RNAiMAX diluted in Opti-MEM I Reduced Serum Medium (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk 0.05% Tween (Sigma) in tris buffered saline for at least 1h ...
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bioRxiv - Neuroscience 2020Quote: ... 2-amino-5-phosphonovaleric acid (APV) (Sigma, A8054, USA), AMPA/kainate receptor antagonist 6-Cyano-7-nitroquinoxaline-2 ...
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bioRxiv - Cell Biology 2020Quote: ... Then 50 μL 5% (v/v) trypsin (Sigma-Aldrich) was added ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 μM Phloretin (catalog # P7912) were from Sigma. Same volume of DMSO was used as control.
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 5 mL red cell lysis buffer (Millipore), and incubated for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 5-Iodo-2’-deoxyuridine (IdU at 25µM – Sigma # I7125) and 5-Chloro-2’-deoxyuridine (CldU at 50µM – Sigma # C6891) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... 5-7 IU of PMSG (Millipore #367222/BioVendor #RP1782721000) was injected (IP ...
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bioRxiv - Immunology 2021Quote: ... or 5 µM AMPK inhibitor (SBI-0206965; Sigma-Aldrich). After 2 h ...