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Citations for Millipore Sigma :
3751 - 3800 of 10000+ citations for PGFM ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... LB-agar plates which contained 100 μg/mL ampicillin (Sigma-Aldrich) were used to grow E ...
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bioRxiv - Cell Biology 2023Quote: ... the source of collagen used to coat the plates (C3867, Sigma) was an essential feature ...
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bioRxiv - Cell Biology 2022Quote: ... LabTek 8-well plates and treated with 2 mM thymidine (Sigma) and 2 μg/ml doxycycline for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... rTDCs were seeded onto 24-well plates (Greiner BioOne, Sigma Aldrich) at a density of 25,000cells/well ...
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Evaluation of mRNA-LNP and adjuvanted protein SARS-CoV-2 vaccines in a maternal antibody mouse modelbioRxiv - Immunology 2023Quote: ... Plates were blocked with 3% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 hour at room temperature and then washed with PBS-T (Phosphate buffered saline [Sigma-Aldrich] with 0.1% Tween-20 [Fisher Bioreagents]) ...
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bioRxiv - Systems Biology 2023Quote: ... We used TLC on Cellulose F glass plates (Millipore, Burlington, MA) to separate anthocyanin pigments in Forestal solvent (glacial acetic acid/water/HCl 30:10:3 ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were pre-coated with Poly-ornithine (Sigma,100 mg/L) to prevent cell detachment ...
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bioRxiv - Cell Biology 2023Quote: ... plates coated with poly-ornithine (20 μg/mL in water, Sigma) and laminin (5 μg/mL in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... plates coated with poly-ornithine (20 μg/mL in water, Sigma) and laminin (5 μg/mL in PBS ...
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bioRxiv - Biophysics 2023Quote: Greiner Sensoplate™ glass bottom 384-well plates (Sigma-Aldrich M4187) were used for visualization of phase-separated droplets in this study ...
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bioRxiv - Genetics 2023Quote: ... Gelatin plates were prepared by heating stock gelatin (Cat# G1393 Sigma) in a 37% water bath ...
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bioRxiv - Microbiology 2024Quote: ... Plates were sealed with a Breathe-Easy gas permeable membrane (Sigma) and statically cultured in the BioTek plate reader at 37° C for as long as needed (usually 72 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Plates were coated with FN (Sigma-Aldrich, Darmstadt, Germany, human plasma) and 5x105 cells were seeded and allowed to attach 5-10 min ...
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bioRxiv - Cell Biology 2024Quote: ... -Trp (-HLT) agar plates containing 3-Amino-1,2,4-triazole (Sigma, A8056) at the indicated dilutions ...
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bioRxiv - Microbiology 2024Quote: ... the plates were than stained with 0.1% w/v safranin (Sigma) for 20 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... and dexamethasone in MS agar plates (50-02-2, Sigma-Aldrich). An equal amount of pure DMSO ...
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bioRxiv - Cell Biology 2024Quote: Cells were detached from the culture plates using Accutase (Sigma#A6964), washed in ice-cold PBS and stained with antibodies diluted in 100ul FACS buffer (PBS containing 2% FBS and 2.5 mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... plates were developed with alkaline phosphatase substrate (Sigma, cat: P7998-100ML) with simultaneous reading on FLUOstar Omega plate reader every 5 mins.
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bioRxiv - Neuroscience 2020Quote: ... sections were washed again in washing buffer (2 × 5 min) before being incubated in the same buffer with 5% bovine serum albumin (BSA, Sigma, A7030) as blocking solution for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended at an OD600 of 1 in 5 ml of agroinfiltration buffer (10 mM MgCl2 and 250 μM of 3’,5’-Dimethoxy-4’-hydroxyacetophenone (Sigma-Aldrich)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked for a minimum of 1 hour at room temperature in 5% (w/v) non-fat milk or 5% (w/v) BSA (A4503-50G; Sigma Aldrich) in TBS-T (1x TBS and 0.1% Tween-20 ...
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bioRxiv - Immunology 2020Quote: ... nitrocellulose membranes were probed with protein-specific primary antibodies diluted in PBS containing 5% Milk or 5% BSA as follows: anti-actin Cat #A5441 from Sigma Aldrich; anti-tubulin Cat #5286 from Santa Cruz ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol (Hyder et al., 1999)) in a volume of 5 µl using the unspecific competitor poly(dI-dC) (Sigma-Aldrich). 20 ng of poly(dI-dC ...
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bioRxiv - Cell Biology 2019Quote: ... For the cold-stable assay cells were briefly rinsed with ice-cold cytoskeleton buffer (CB buffer; 10 mM MES, 150 mM NaCl2, 5 mM MgCl2, 5 mM glucose; Sigma-Aldrich), fixed with an ice-cold glutaraldehyde-based solution in CB buffer (0.05 % glutaraldehyde ...
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bioRxiv - Cancer Biology 2019Quote: ... the PDCD4 shRNA oligonucleotides (5‘-CTGGACAGGTGTATGATGTGG-3’) were synthesised and cloned into the MISSION® TRC2 pLKO.5-puro Empty Vector (SHC201, Sigma) by TsingKe Ltd ...
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bioRxiv - Developmental Biology 2020Quote: Female 7-12-week-old FVB/NJ mice were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PSMG) (ProSpec HOR-272) followed by 5 IU human chorionic gonadotropin (hCG) (Sigma CG10) 48 hours later.
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bioRxiv - Genomics 2021Quote: ... The tissue was mechanically lysed with a scalpel in a total volume of 5 ml lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115] ...
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bioRxiv - Biochemistry 2020Quote: ... HLCs were exposed to either 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5′-monophosphate (AICAR; 1mM; Sigma-Aldrich, A1393-50MG), O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA ...
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bioRxiv - Neuroscience 2020Quote: ... retinas were washed during 5 minutes in PBS and permeabilized overnight at 4°C in 5% normal goat serum (NGS, Sigma Aldrich) and 1% Triton-X100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... flies were collected 2-5 days post eclosion and grown for another 3-5 days on 1mM all-trans retinal (R2500; Sigma-Aldrich) supplemented food in complete darkness before experimental testing was performed ...
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bioRxiv - Molecular Biology 2021Quote: ... 45 μL of this solution was removed and added to either 5 μL of low TE or 5 μL of 10% Triton-X (X100-500ML, Sigma Aldrich). These two specimens constitute the undiluted eluate from the dry swabs.
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bioRxiv - Neuroscience 2020Quote: ... Neurons were cultured at 37°C/5% CO2 and glial growth was inhibited at DIV4 by addition of 5-fluoro-2’-deoxyuridine (30 μM)(Sigma, F0503). A 50% fresh culture medium change was performed every 4 days.
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bioRxiv - Neuroscience 2020Quote: ... which were then plated in black 96-well plates in the presence of 5 mM complex I substrates glutamate and malate with or without 5 µM CCCP (carbonyl cyanide m-chlorophenyl hydrazone, Sigma-Aldrich) and 1 µM TMRM (tetramethylrhodamine ...
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bioRxiv - Microbiology 2021Quote: ... the membranes were transferred to sterile containers and washed with 5 ml of TSB supplemented with 5 µg/ml of rifampicin (Sigma-Aldrich). Next ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl cells were spread on a 1 mm glass slide pre-treated with 5 μl poly-L-lysine (Sigma Aldrich). A Nikon 9Oi eclipse microscope was used to capture differential interference contrast (DIC ...
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bioRxiv - Immunology 2020Quote: ... Red blood cells were then lysed by incubating for 5 minutes at room temperature in 5 ml of lysis buffer [0.864% ammonium chloride (Sigma-Aldrich: A0171) diluted in sterile de-ionized H2O)] ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Microbiology 2021Quote: ... gondii parasites were cultured in complete medium (DMEM, +5 % FCS) or in custom culture medium −Pan (DMEM −Pan, +5 % dialyzed FBS) supplemented with Val (Sigma, V6504), pantoate (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were re-suspended in 5 ml washing buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115] ...
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bioRxiv - Biophysics 2020Quote: ... 5 μl washed beads were added with 5 μl of 0.5 mg/ml lectin (lectin from Phaseolus vulgaris, Sigma L2646-25MG) and incubated at 4 °C for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... specific siRNAs (si-HER3-1: 5’-GCUGAGAACCAAUACCAGA, si-HER3-2: 5’-CCAAGGGCCCAAUCUACAA) and a validated control siRNA were obtained from Sigma-Aldrich.
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bioRxiv - Bioengineering 2022Quote: ... were digested overnight in papain digestion buffer (containing 100 mmol phosphate buffer, 5 mmol L-cystein, 5 mmol EDTA and 140 μg/ml papain (P4762, Sigma-Aldrich)) at 60 °C ...
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bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then incubated in a blocking buffer (5% normal goat serum, Vector; 5% normal donkey serum, Wako; 3% BSA, Sigma-Aldrich; and 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2020Quote: ... LdBob promastigotes were routinely maintained at 26 °C in 5% CO2 and cultured in Dulbecco’s Modified Eagle-Leishmania (DME-L) medium supplemented with 5% SerumPlus™ (SAFC BioSciences/Sigma Aldrich, St ...
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bioRxiv - Biophysics 2021Quote: ... was performed using a pre-annealed DNA-RNA template (DNA45: 5’-TTCTTT ATCTCTTATTTCCTTCTATTTTCCACGCGCCTTCTATTT-3’; RNA13: 5’-[6FAM]-GGCGCGUGGAAAA-3’, Sigma Aldrich). The reaction buffer comprised 25 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 24 h at 60 °C in papain digestion solution containing 125 μg/ml of papain with 5 mM L-cysteine-HCl and 5 mM EDTA in 100 mM Na2HPO4 (all from Sigma, USA). The DNA content was measured using the Picogreen assay ...
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bioRxiv - Neuroscience 2019Quote: ... KO2 along with Cas9 donor plasmid were used as template DNA and amplified with F 5’-GAAGAAGAATGGCCTGTTCG-3’ and R 5’-GCCTTATCCAGTTCGCTCAG-3’ with KOD Xtreme (Novagen, #71975). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... metabolic activity by the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)-carbonyl-2H-tetrazoliumhydroxidand (XTT; Sigma-Aldrich, USA) reduction assay ...