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Citations for Millipore Sigma :
3801 - 3850 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Biochemistry 2023Quote: ... Afterwards, membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... MyoII:3xmKate2 or CellMask Orange Plasma membrane Stain (ThermoFisher; C10045; diluted 1:10 000 in ex vivo culture medium (Schneider’s medium (Sigma-Aldrich) supplemented with 10% Fetal bovine serum (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM total lipid mixtures were extruded through a 0.05 μm pore size 19 mm polycarbonate membrane (Sigma, cat#WHA800308) with filter supports (Avanti ...
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bioRxiv - Biochemistry 2023Quote: ... Ninety-six randomly selected clones from this transformation were grown in 500 µL AIM supplemented with 50 µg mL−1 kanamycin for 48 hours at 20 °C in 96-deep well plates sealed with Breathe-Easier membranes (Sigma). The culture medium was then separated from the cells by centrifugation for 15 minutes at 4500 x G and 10 µL of culture supernatant was assayed for xylobiose lysis using the protocol detailed in Controls for the detection of CAZyme activities in coupled reactions.
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bioRxiv - Microbiology 2023Quote: ... After blocking with 5% BSA the membrane was incubated for 1 h with 50 µg of purified PLG (Sigma-Aldrich) diluted in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and further incubated with secondary antibody (goat anti-mouse AP, 1:15000; Sigma Aldrich, St. Louis, MO). The membrane was washed with TBS-Tween 20 (TBS-T ...
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bioRxiv - Immunology 2023Quote: ... the membranes underwent a 30 min-blocking at RT using 1x carbo-free blocking solution (Vector) containing 1% Tween-20 (Sigma). The membranes were then blotted with 1 μg/mL biotinylated Sambucus Nigra Lectin (SNA ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated 1 hour at 25°C with a second antibody (anti-mouse HRP conjugated antibody; Sigma) diluted 1:10,000 in PBS-Tween ...
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bioRxiv - Neuroscience 2023Quote: ... dissociated hippocampal cells were plated on 1-μm pore and 30-mm diameter polyethylene tetra-phthalate (PET) membrane filter inserts (Millipore). These inserts were matrix-coated with poly-L-lysine and laminin on both the upper and lower surfaces ...
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bioRxiv - Neuroscience 2020Quote: ... primary rat neurons were washed with TM and then incubated with phenol red free TM containing WST-1 reagent (Sigma, Cat# 5015944001, 20 μl/ml) for 4 h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Urine samples were aliquoted into 96-well plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panel 1 (EMD Millipore Corporation, Charles, MO), and analyzed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 12] Urine samples were aliquoted into 96-well plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panels 1 and 2 (EMD Millipore Corporation, Charles, MO), prepared and analyzed according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2019Quote: ... The silver stained protein spots were de-stained using the ProteoSilver Plus Stain Kit (Sigma-Aldrich, MO, USA), and in-gel enzymatic digestion was done overnight at 37°C using Trypsin Singles ...
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bioRxiv - Physiology 2019Quote: ... Gels containing protein spots were then stained using the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich, MO, USA) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RIP assay and RNA pull down assay were performed using RIP RNA-Binding Protein Immunoprecipitation kit (Sigma, USA) and Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The extraction of nuclear proteins has been performed using the CelLytic™ NuCLEAR™ Extraction kit (Sigma-Aldrich) complemented with PIC ...
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bioRxiv - Physiology 2020Quote: ... Subcellular fractions were extracted using differential lysis and solubilization with a commercial Millipore Compartmental Protein Extraction Kit (Millipore) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Lactate and pyruvate were measured in the protein-depleted flow-through using assay kits (MAK064-1KT, Sigma-Aldrich) and (ab65342 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant and standards containing bovine serum albumin (BSA) were analyzed using a BCA protein assay kit (Sigma).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Lipid profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: IGF1 levels were measured in serum and SSPC culture supernatant using ELISA (Sigma-Aldrich, RAB-0229) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... as well as BG505 Env-NP expression by ELISA (NP capture: Galanthus nivalis lectin (GNL, Sigma) / NP detection ...
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bioRxiv - Immunology 2020Quote: ... The pro-inflammatory cytokines in the supernatant were analyzed by multiplex ELISA (Millipore, HCYTMAG-60K-PX29) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Plasma cytokines and cytokines secreted in cell culture media were measured using ELISA (Luminex 200; Millipore) by following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Nunc 96-well ELISA plates were coated with 50 µl of 2 µg/ml SA (Sigma) in borate saline buffer (100 mM boric acid ...
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bioRxiv - Neuroscience 2022Quote: ... sandwich ELISA of tau-5 coating antibody (20 µg/ml; Millipore Cat# 577801-100UG RRID: AB_212534) with either biotin-conjugated HT-7 (for human tau ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed in ELISA wash buffer (0.01M PBS with 0.05% Tween 20; Sigma; P7949) for 4x 2 minutes on an orbital shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysate was added overnight to an ELISA plate precoated with anti-Itgb1 antibody (Sigma#MAB1997), the plates were then washed and the Itgb1 levels measured upon addition of HRP-streptavidin using a microplate reader at 405 nm with ABTS substrate ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
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bioRxiv - Neuroscience 2019Quote: ... and visualized by exposing the membrane to a charge-coupled device camera (LAS1000, Fujifilm, Tokyo, Japan) using a chemiluminescence kit (Merck Millipore, Billerica, MA, USA). Membranes were stripped and reprobed for β-actin (diluted 1:50 000 ...
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bioRxiv - Cancer Biology 2022Quote: ... donor cells were labelled with CellVue Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labelling (Sigma Aldrich, Cat#MINCLARET-1KT) following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was equally adjusted using Bradford protein assay (Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Plant Biology 2020Quote: Total protein extract from the different plants was separated on 10% SDS-PAGE gel and transferred to a PVDF membrane (Immobilon-P Transfer membrane, Millipore) by semi-dry blotting in 25 mM Tris–HCl ...
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bioRxiv - Cell Biology 2022Quote: ... Nonspecific binding sites on the membrane were blocked by incubating the membrane in 5% milk powder in PBS containing 1X Tween-20 (Sigma). The membrane was then incubated with the primary antibody of interest at 40C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... dissected hippocampi were cut into 400 μm pieces with a tissue chopper and placed on a porous membrane (2 slices per membrane, Millicell CM, Millipore) in six-well plates ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... and filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet ...
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bioRxiv - Developmental Biology 2022Quote: ... separated on 15% sodium dodecyl sulfate-polyacrylamide electrophoresis gels and transferred to a polyvinylidene difluoride membrane (Immobilon-P Transfer membrane, Millipore) by wet blotting in transfer buffer (25 mM Tris– HCl ...