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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Molecular Biology 2020Quote: Premature chromosome condensation was induced using the protein phosphatase 1 inhibitor calyculin (Sigma, C5552-10UG). To determine the cell cycle stage of prematurely compacted chromosomes ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The proteins were then transiently transfected (pBiex-1; Escort IV, Sigma-Aldrich, St. Louis, MO) into Sf9 cells ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated for 45 min with 1%v/v Benzonase Nuclease (SIGMA E1014). For the FASP digestion ...
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bioRxiv - Physiology 2021Quote: ... Japan) and anti-glial fibrillary acidic protein (GFAP) (1:2,000; mouse monoclonal, G3893, Sigma-Aldrich) were used to assess activated microglia and reactive astrocytes in ischemic regions ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was eluted in buffer B with 0.5 M KCl and 1 mM methotrexate (Sigma). Then the protein was precipitated with 40% TCA for 60 minutes on ice followed by centrifugation (10000 g ...
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bioRxiv - Biochemistry 2020Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 30°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... After that 50 µl of 1:5 dilution Protein A colloidal gold conjugate (Sigma, USA), which is used as an indicator was added to the test hole and was allowed to soak for 2 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to 1 mg/ml using a 50,000 MWCO Amicon Concentrator (Millipore), flash frozen and stored at -80°C.
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bioRxiv - Physiology 2022Quote: Protein samples were solubilized in 1% NP-40 lysis buffer with 4% protease inhibitors (Sigma) and centrifuged for 14,000 g for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
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bioRxiv - Molecular Biology 2019Quote: RNA-renatured proteins (~40 μg) were supplemented with 1 μg anti-NF-H (N4142, Sigma), 1 μg anti-Tau (T9450 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 30K (Merck Millipore) and stored at -80°C.
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 100K (Merck Millipore) and stored at -80°C ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... wild type and mutant proteins were treated similarly with calpain 1 (Sigma-Aldrich, Cat. #C6108) in vitro and their cleavage was assessed by immunoblotting.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using primary antibodies α-FLAG (monoclonal α-FLAG M2, Sigma, 1:1000) or α-Myc (Clone A-14 ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was briefly vortexed in the presence of 1% Triton X-114 (Sigma), incubated on ice for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein levels were determined by immunoblotting using anti-FLAG clone M2 (Sigma, F3165, 1:3000).
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression in E.coli was stimulated by IPTG (Isopropyl β-D-1-thiogalactopyranoside, Sigma-Aldrich) at 1 mM at 16°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich, I6758) for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Flag-hSpt16 proteins were incubated with 12.5 U of recombinant ULP-1 SUMO Protease (Sigma) for 16 h at 4°C to remove SUMO moieties.
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of protein was incubated with 20 mM methoxypolyethylene glycol maleimide (mPEG, Sigma-Aldrich) in the presence of 3% SDS for 10 minutes at 60ºC ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 1 h incubation with Protein G agarose beads (Millipore, catalog no. 16-266) at 4 °C with constant rotation ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated protein (7-15 mg/ml) was serially diluted in 1% Genapol X-100 (Sigma). 0.3-0.5 μl of diluted protein was added to the cis (ground side ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were concentrated to 1 mL with a 30K MWCO concentrator (EMD Millipore) and diluted with 1 mL buffer A ...
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bioRxiv - Microbiology 2024Quote: ... protein expression was induced by adding 50 μM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma) and 1 mg/mL riboflavin (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated with addition of Trichloroacetic acid (TCA) 100% (1/5v/v) (Sigma #T6399) and washed with 0.01M HCl/ 90% Acetone before by peptide identification by LC/MS.
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bioRxiv - Pathology 2021Quote: α-synuclein was fixed to membranes by boiling for 10 minutes in PBS before membranes were washed in 0.05 % Tween 20 (Sigma) containing Tris-buffered saline (TBST ...
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bioRxiv - Bioengineering 2021Quote: Membranes used for the microporous membrane scaffold method were 0.45 µM pore size and 47 mm diameter (Sigma-Aldrich). Prior to sampling ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were diluted to a final concentration of 1 × 106 CFU/mL in sterile 1X PBS and were stained in the dark for membrane potentials using 30 μM membrane potential indicator dye DIO2(3) (3,3=-diethyloxacarbocyanine iodide) (320684; Sigma) for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h at 50 V followed by 3.5 h at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
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bioRxiv - Genomics 2019Quote: ... for 1 hour at 50 V followed by 3.5 hours at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 72h on porous membranes (Whatman ® Nucleopore™ Track-Etched Membranes WHA150446, Sigma Aldrich, St. Quentin Fallavier, France) in 4-well plates [21] ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... were injected in the MGE of E14.5 brain slices placed on supporting membranes (Whatman nucleopore track-etched membranes, Sigma). Electroporation was conducted with platinum electrodes (ΒΤΧ Harvard Apparatus ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... followed by transfer onto methanol-activated polyvinylidine fluoride (PVDF) membrane (Immobilon-FL membrane, pore size 0.45 μm; Millipore, IPFL00010). Membrane was blocked ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Biophysics 2024Quote: ... spread on a conjugation membrane (mixed cellulose ester membrane from Millipore, 47 mm diameter, and 0.45 μm pore size) over an LB+agarose+DAP+Ara Petri dish and incubated 3 hours for conjugation and recombination to take place ...
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bioRxiv - Molecular Biology 2024Quote: SDS-PAGE (10%) was followed by a semidry blotting method using PVDF membrane (Millipore Immobilon-P membrane, 0.45 µm). For blocking 5 (m/v ...
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bioRxiv - Neuroscience 2019Quote: ... After protein transfer blots were blocked in 5% BSA/TBS for 1 h and stained for Sox2 (1:100, Millipore AB5603), Sox9 (1:250 ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Plant Biology 2021Quote: ... 17 µL of FLAG-tagged effector protein extract was added to the beads with 1 µL of 1 mM acetyl-CoA lithium salt (Sigma-Aldrich) and 2 µL of 1 µM IP6 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... KLH protein was dissolved in PBS to 5 mg/mL of and then mixed 1:1 with CFA (both Sigma-Aldrich) upon which 40 μL of the immunization mixture was injected in footpads and flanks of draining popliteal and inguinal LNS ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were diluted to a 1 mg/mL protein concentration using 50 mM ammonium bicarbonate and incubated overnight with trypsin (1:200 v/v, Sigma #T6567). Digested samples were acidified and urea removed using a Sep-Pak C18 96-well plate (Waters #186002320) ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...