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Citations for Millipore Sigma :
3751 - 3800 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... GFAP and Iba-1 protein analyses were generated from previously collected data and normalized to GAPDH (1:5000; cat# G9545, Sigma-Aldrich) [13] ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated with anti-rabbit IgG antibody conjugated to horseradish peroxidase (Cat # A9044, Sigma Aldrich; 1:10,000 dilution) for 1 hr at RT and proteins visualized using Clarity ECL western blotting substrate (BioRad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three more washing steps with TBS were performed before resolving the staining with 5 ml of substrate per membrane: a 30 mg tablet of 4-chloro-1-naphthol (Sigma) dissolved in 10 ml of methanol ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were further incubated with horseradish peroxidase-conjugated secondary antibody for 1 h and reacted with ECL (Millipore, USA). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred into polyvinylidene difluoride (PVDF) membrane in 1× Transfer buffer (Tris-base/Glycine/Methanol, Sigma-Aldrich/Merck, Poznan, Poland) using the Mini Trans-Blot® system (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... Membranes were subsequently incubated overnight at 4 °C with primary antibodies (1:2,000 monoclonal mouse anti-TRPA1 antibody, Sigma-Aldrich, and 1:3,000 MF20 mouse anti-myosin heavy chain antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were incubated with antibodies (total anti-HyYap serum was used at 1:1000 dilution, anti-actin (clone C4, Millipore) at 1:2000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... were fractionated by SDS-Page 10% and transferred to nitrocellulose membrane for immunoblot analysis using rabbit anti-GRP78 antibodies (GL-19, 1:5000 dilution from Sigma), anti-PERK antibodies (CS56833 ...
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bioRxiv - Neuroscience 2019Quote: ... and corresponding PVDF membranes were incubated overnight at 4°C with primary antibodies: mouse anti-ATXN3 (1H9) (1:2000, MAB5360, Millipore), rabbit anti-MJD [22] (1:20000) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 ml/well of this medium was added to wells in a 24 well plate and Isopore Membranes (EMD Millipore) were suspended at the surface of the medium to create an air-liquid interface ...
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bioRxiv - Neuroscience 2019Quote: Membranes were blotted with primary antibodies including rabbit polyclonal anti-GluN1 C- terminus (1:2000, Sigma-Aldrich Cat# G8913, RRID:AB_259978), rabbit polyclonal anti-GluN2A N-terminus (1:500 ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... the NC membranes were incubated with goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP; Sigma-Aldrich; dilution, 1:8000) and the color reaction was examined using ECL Kit (Amersham Pharmacia Biotech ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then stained and visualised for β-actin (rabbit anti-β-actin HRP, Sigma-Aldrich, A3854, 1:50,000) as described above.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Membranes were probed with primary antibodies targeted against tyrosine hydroxylase (TH, 1:1000 dilution, EMD Millipore #AB152, Burlington, MA, USA) or β-Actin (loading control ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... crosslinked to the membrane by chemical treatment at 60°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma) for 1 h 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was transferred to Hybond Nx nylon membranes (Amersham) and crosslinked using EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Sigma) (Pall & Hamilton 2008) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Linear pST76-A plasmid was ligated with the PCR fragment using T4 ligase enzyme overnight at 16 °C followed by heat inactivation at 65 °C for 10 minutes and dialysis for 1 h on a 50 nm pore-sized membrane (Millipore). The ligation product was transformed into E ...
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bioRxiv - Molecular Biology 2021Quote: ... The ionic liquid solution was dialyzed into lysis buffer (6 M urea, 2 M thiourea, 2% CHAPS, 1% DTT) using a 3k MWCO cellulose membrane (Amicon Ultra, Millipore).
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bioRxiv - Neuroscience 2020Quote: ... the membranes were washed 3x in TBST (total wash time 1 hr) and were developed with luminata crescendo ECL substrate (Millipore) on an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was blocked for 15 minutes at room temperature in TBST containing 1 % BSA before adding HRP-Streptavidin (Sigma) to 1:20000 dilution and incubating for further 30 minutes ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated in primary antibodies (1:1000) overnight at 4° in 5% bovine serum albumin (BSA) (Sigma, A9647-100G), washed with TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich). They were passaged every two to three days in an appropriate ratio ...
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bioRxiv - Neuroscience 2020Quote: ... gels and transferred (30 V; Thermo-Fisher mini-blot wet transfer system; 1 hr) to Immobilon FL polyvinylidene fluoride (PVDF) membranes (Millipore) for immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... 30 μl of extract was resolved on 10 or 12% SDS-PAGE gels and after transfer the membranes were probed with 1:1000 dilution of anti-HA antibody (Sigma). Imaging of blots was done by Biorad ChemiDoc ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-RELN G10 (MAB5364, Millipore 1:2000) or 142 (for human RELN ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was run on 12% acrylamide SDS-PAGE gel at a final concentration of 1 µM and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) using a semi-dry transfer apparatus ...
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bioRxiv - Cell Biology 2019Quote: ... the membrane was incubated with either α-D-TPX2 antibody at 1:2000 dilution or α-tubulin (DM1-A, Sigma) antibody at 1:10,000 dilution for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 h at room temperature in 5% non-fat dry milk (Marvel) or 5% BSA (Sigma) in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on a gel were transferred to a nitrocellulose membrane and analyzed by using the antibodies at 1:3000-10,000 dilutions: DM1a (Sigma, #T9026), α-GFP (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Microbiology 2020Quote: ... Water and biofilms from the outflow were collected in a sterile 1 L bottle and filtered through a 0.22 μm nitrocellulose membrane (Millipore, USA). The membrane was transported to the laboratory on ice and stored at 4 °C for bacterial enrichment into 10 mL of Nutrient Broth (Biolife ...
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bioRxiv - Microbiology 2020Quote: ... The PVDF membrane was blocked for 1 h in PBS supplemented with 0.1 % Tween-80 from Sigma Aldrich (‘PBS-Tween’) and 4 % of milk powder ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then blocked for 1 hour at RT using 5% milk in TBS with 0.025% Tween 20 (Sigma, P1379) (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed and incubated with primary antibody diluted in 5% BSA/TBST (1:10,000 anti-Flag (Sigma-Aldrich) or anti-Beta lactamase (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578 ...
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bioRxiv - Cell Biology 2022Quote: ... mitochondria enriched heavy membrane fractions (200 μg) from control fibroblasts were chemically cross-linked with 1 mM dithiobis-sulfosuccinimidyl propionate (DSP) (Sigma) in HIM buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were washed with TBS-T and membranes were incubated with secondary antibodies anti-mouse and anti-rabbit coupled to alkaline phosphatase (1:5000; Sigma) diluted in TBS-T/1% BSA for one hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Neuroscience 2022Quote: ... dissociated hippocampal cells were plated onto 1-μm pore and 30-mm diameter polyethylene tetra-phthalate (PET) membrane filter inserts (Millipore) that were matrix-coated with poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes previously incubated with anti-RplA were incubated with the secondary antibody anti-rabbit IgG-HRP (#A8275, 1:10000, Sigma) for 1 h at room temperature and then washed three times with T-PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... An equivalent of 1 μl of the original IVT was used for TCA precipitation and filtered onto a GF/C membrane (Millipore). The cap-analogue beads were washed 5 times with binding buffer and the final bead fraction suspended in SDS-PAGE sample buffer ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... mitochondria enriched heavy membrane fractions (200 μg) from control fibroblasts were chemically cross-linked with 1 mM dithiobis-sulfosuccinimidyl propionate (DSP) (Sigma) in HIM buffer ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Afterwards, membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...