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Citations for Millipore Sigma :
3551 - 3600 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... were either stored frozen at –80°C or resuspended in 1 ml of 8 M guanidine HCl (Sigma) in refolding buffer (250 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in 9.5 ml GBSS containing 0.3% bovine serum albumin (BSA) and 8 ml 28.7% Histodenz solution (Sigma-Aldrich) to reach an approximate density of 1.070 ...
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bioRxiv - Biochemistry 2024Quote: ... These cells were transduced with the lentiviral particles using Polybrene (8 μg/ml; Sigma-Aldrich TR-1003-G). Puromycin (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... the fermented PKM was mixed with 8 ml phosphate-buffered saline (PBS) followed by filtration through miracloth (Sigma) to remove PKM debris and collect bacterial cells for RNA extraction ...
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bioRxiv - Synthetic Biology 2024Quote: ... the fermented PKM was mixed with 8 ml phosphate-buffered saline (PBS) followed by filtration through miracloth (Sigma) to remove PKM debris and collect bacterial cells for RNA extraction ...
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bioRxiv - Developmental Biology 2024Quote: ... and non-mitochondrial respiration was measured following injection of 6.25mM sodium azide (Sigma-Aldrich, CAS 26628-22-8). Basal OCR was measured for 8 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... 8-week-old C57BL/6J mice received an intraperitoneal injection of 75 mg/kg methimazole (MMZ (EMD Millipore)) ...
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bioRxiv - Immunology 2024Quote: The enzymatic activity of the cultures after exposures +/-CAS was determined using the WST-8 assay (96992; Sigma). Culture medium in the OrganoPlate was replaced with 25 µL WST-8 solution diluted 1:11 in assay medium in each in- and outlet of the top and bottom perfusion channel ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells were transduced once with lentiviruses supplemented with 8 μg/mL polybrene (#TR-1003-G, Sigma-Aldrich) and selected with puromycin (2 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... target cells were incubated overnight with lentivirus-containing medium with 8 µg/ml polybrene (Sigma, TR-1003-G). After 48 hours ...
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bioRxiv - Neuroscience 2024Quote: Progesterone stock solution (800 µg/mL) was prepared by dissolving 8 mg of progesterone (Sigma, Catalog No. P0130) in 1 mL ethanol followed by 1:10 dilution in sesame oil ...
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bioRxiv - Bioengineering 2024Quote: ... Mw 24,000-38,000Da); and poly(vinyl alcohol) (PVA; Mowiol 8-88; Mw ∼67,000Da) were both purchased from Sigma-Aldrich. Normal rat serum and pooled CSF collected from Sprague-Dawley rats were obtained from Invitrogen and BioIVT (USA) ...
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bioRxiv - Microbiology 2024Quote: ... the animal’s drinking water was replaced from days 3-8 with water containing 5% L-arabinose (Sigma Aldrich). InducTn-seq samples were collected 8 days post inoculation by plating fresh feces on solid LB containing 50 µg/ml kanamycin ...
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bioRxiv - Cell Biology 2024Quote: ... mice aged 8-12 weeks old mice were given 4 doses of 160 mg/kg tamoxifen (Sigma, T5649) dissolved in corn oil (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... cells were pelleted and resuspended in 250 μL lentivirus and 8 μg/mL DEAE-Dextran (Cat# D9885, Sigma) in a total volume of 1.7 mL ...
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bioRxiv - Biophysics 2024Quote: ... 90,000 U2OS cells/well were seeded in 6-well with 8 ug/ml polybrene (Millipore #TR-1003-G) addition to the growth medium and infected with 4ul of lentiviral particle containing supernatant ...
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bioRxiv - Immunology 2020Quote: ... were cultured at a concentration of 1 million cells per ml in complete media on pre-incubated anti-CD3/anti-CD28 bound plates in IL-2 (20ng/ml) (Sigma-Aldrich) for 2 days and then just IL-2 (20ng/ml ...
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bioRxiv - Physiology 2019Quote: The interactions between TRPA1 and IL-R1 in nodose ganglia cultures were detected by PLA with Duolink in situ kit (Sigma- Aldrich). Primary nodose ganglion cells were harvested and cultured on glass coverslips as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 105 IL-7 dependent primary Irf4−/− preB-I cell cultures were resuspended in 400 µL RPMI medium (D5030, Sigma-Aldrich) with 600 µL viral supernatant and 1.5 µL polybrene and spun in culture plates at 2700 RPM for 90 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: Fluorescent immunostaining for nuclear NFκB and cytoplasmic NFκB was quantified in immortalized C8D30 astrocytes following 2-hr treatment with 1μg/ml LPS or a cocktail of 3 cytokines: Il-1α (3ng/ml, Sigma, Cat# I3901), TNFα (30ng/ml ...
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bioRxiv - Neuroscience 2021Quote: ... the reporter cells were exposed to drugs or DMSO vehicle for one hour before the addition of 3 ng/mL IL-1a (Sigma, I3901), 400 ng/mL C1q (MyBioSource ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... PBMCs cultures not supplemented with IL-2 overnight were stimulated with 25 ng/ml phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich) and 1 μg/ml ionomycin (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... LP cells were incubated for 5 h with 20 ng/ml PMA and 1 μg/ml ionomycin in the presence of 10 μg/ml Berfeldin A (Sigma, IL). For FACS analysis ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA was isolated from the si-NC and si-Dnmt3aos-transfected M(IL-4) macrophage cells and was then treated with bisulfite using the Imprint DNA Modification Kit (Sigma-Aldrich). Bisulfite sequencing and pyrosequencing were conducted by Shanghai Sangon Biotech Corporation ...
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bioRxiv - Microbiology 2021Quote: ... All SEC-FPLC described in this study was performed on a GE Healthcare AKTA PV-908 FPLC system (Chicago, IL) on Superdex S200 10/300 GL (Sigma-Aldrich) and Superose 6 10/300 GL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently transduced twice with viral supernatant within 48 hrs in the presence of 50 IU/mL IL-2 and 6 mg/mL polybrene (Sigma-Aldrich). TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells ...
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bioRxiv - Bioengineering 2021Quote: A1 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1α (3 ng/ml, Sigma, I3901), TNF-α (30 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) added to the wells ...
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bioRxiv - Immunology 2022Quote: ... All cocultures were maintained in SCGM supplemented with IL-2 (120 IU/ml) and in the presence of 10 µM raltegravir (Sigma-Aldrich) and 0.4 µM nevirapine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Neuroscience 2024Quote: The viral particle stock was diluted in sterile saline (0.9% NaCl) (Hospira, Lake Forest, IL) with 0.01% Fast Green (Sigma, St. Louis, MI). A 10-µl Hamilton syringe (Hamilton Company ...
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bioRxiv - Cell Biology 2020Quote: A2780 human ovarian cancer cells were maintained in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then suspended in veronal buffer and human serum (Sigma-Aldrich, H4522) (or heat-inactivated for 30min at 56°C) ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology ...
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bioRxiv - Molecular Biology 2021Quote: The gene of human GAPDH was cloned into pET-28a(+) expression vector (Novagen) with an N-terminal His-tag followed by the Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Cell Biology 2022Quote: ... dried and incubated with 10 μg/mL of IgG from human serum (Sigma) in PBS for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232).
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bioRxiv - Molecular Biology 2021Quote: ... bleomycin and human activating FAS (anti-FAS) antibody were purchased from Millipore Sigma. PGE2 ...
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bioRxiv - Immunology 2022Quote: ... media was replenished with RPMI containing 5% type AB human serum (Sigma-Aldrich). For single-round experiments with VSV-G-pseudotyped viruses ...
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bioRxiv - Systems Biology 2019Quote: ... The human plasma reference standard NIST SRM 1950 was obtained from Sigma Aldrich. Human cancer cells (HeLa S3 ...
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bioRxiv - Cell Biology 2020Quote: ... in acetic acid (0.02 N) or human plasma fibronectin (5 µg/mL; Millipore) in PBS overnight at 4°C and rinsed with imaging medium to remove the coating solution ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were blocked with human IgG (1.25 mg/mL, 15 min; Sigma Aldrich). Surface staining of unfixed/unpermeabilized cells employed the following antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... PINK1 knockdown was performed using MISSION® esiRNA human PINK1 (EHU057101, Sigma Aldrich). Mff knockdown (SI Fig ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Sections were incubated with rabbit anti-human-IgG (1:100, Sigma, SAB3701291, IgG) and with rabbit anti-human IgM (1:250 ...
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bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
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bioRxiv - Cell Biology 2019Quote: ... Staining for murine and human MSCs was performed using Oil Red O (Sigma) stain for adipogenesis ...
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bioRxiv - Cell Biology 2020Quote: The human ovarian cancer cell lines COV318 (purchased from ECACC, via Sigma-Aldrich) and SKOV3 (courtesy of Dr Florence Delie ...