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Citations for Millipore Sigma :
3701 - 3750 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Proteins were digested on-bead with 1 µg of trypsin (Sigma Aldrich, Cat# 6567) in 50 µL ABC buffer with rotation at 37℃ overnight ...
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bioRxiv - Systems Biology 2020Quote: Proteins from each sample were subjected to reduction using 5 mM DTT (Sigma-Aldrich) at 60°C for 20 min followed by alkylation using 10 mM iodoacetamide (Sigma-Aldrich ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... The equal amount of proteins were incubated with streptavidin-agarose slurry (Millipore, #16-126) at 4°C for 16 h in order to pull down all biotin-labeled proteins ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred to Immobilon-PSQ PVDF membrane (0.2 μm pore size, Millipore Sigma) by electrophoresis at 100 V for 30 minutes in Tris-Glycine/Methanol buffer ...
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bioRxiv - Microbiology 2021Quote: ... followed by membrane protein cross-linking with 3,3’-dithiobis(sulfosuccinimidyl propionate) (Sigma; 1 mM), at low temperature ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
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bioRxiv - Physiology 2021Quote: ... using the following primary antibodies: glial fibrillary acidic protein or anti-GFAP (Millipore, MAB360). Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A. ...
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bioRxiv - Microbiology 2021Quote: ... Digested proteins were analyzed by Western blotting with anti-M2 FLAG monoclonal antibody (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed and proteins were detected with Immobilon Western chemiluminescent HRP substrate (Millipore) using the ChemiDoc XRS+ Imaging System.
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bioRxiv - Cancer Biology 2020Quote: ... Protein turnover was analyzed by addition of Emetine (100 μM, Sigma Aldrich, Cat# E2375) and RNA decay by addition of Actinomycin D (5μM ...
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bioRxiv - Cancer Biology 2020Quote: RIP assays employed the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: Extraction of protein from isolated exosomes was performed using SDS2.5%/8M urea (Sigma-Aldrich) lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins were concentrated using a 50K MWCO concentrator (EMD Millipore) to 1 mL ...
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bioRxiv - Cancer Biology 2022Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was first purified by immobilized metal-affinity chromatography (IMAC) using Ni2+ resin (Sigma), in a buffer consisting of 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using an Amicon centrifugal concentrator (EMD Millipore, Burlington, MA, USA) and then used for intasome assembly or flash frozen in liquid nitrogen and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore; IPVH00010). After blocking with 5 % skim milk ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore) or Microsep™ Advance 3kDa (PALL ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore, Bedford, MA, USA). The relative intensity of the blot was measured using the ImageJ software and was normalized by the intensity of β-actin blot.
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were subjected to SDS-PAGE and transferred to Immobilon-P membranes (Millipore). Membranes were immunoblotted with the indicated antibodies and developed with ECL (Thermo Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were reduced by addition of 5 mM DTT (Merck Sigma, Cat. No. 43815) or 5 mM TCEP (Merck Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were loaded to a filter (Merck-Millipore, Microcon 10 kDa, Cat. No. MRCPRT010) after bead beating and reduced by addition of DTT and alkylated with iodoacetamide ...
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bioRxiv - Developmental Biology 2022Quote: ... The collected CM was concentrated by protein precipitation with 80%-saturated ammonium sulfate (Sigma) and dissolved in phosphate-buffered saline without calcium and magnesium [PBS (-)] supplemented with 0.1% Triton X-100 and a proteinase inhibitor mixture [0.2 mM 4-(2-aminoethyl ...
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bioRxiv - Microbiology 2022Quote: ... the respective proteins were overexpressed in BL21 DE3 cells carrying a pET28a vector (Novagen) with an (indirectly ...
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bioRxiv - Developmental Biology 2022Quote: ... The collected CM was concentrated by protein precipitation with 80%-saturated ammonium sulfate (Sigma) and dissolved in 50 mM Tris-HCl (pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... total protein lysates were instead incubated overnight with Anti-FLAG M2 Magnetic Beads (Sigma) or G-coupled magnetic beads ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then transferred onto a PVDF transfer membrane (Immobilon, Millipore, Bedford, MA). The presence of transferred proteins on the membrane was confirmed by staining with Ponceau S (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... The flow-through containing the target protein was concentrated (Amicon 50 kDa cutoff, Millipore) and loaded onto a size-exclusion column (SRT-3C SEC-300 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins collected from size-exclusion chromatography were concentrated (Concentrators, Amicon Ultra, Millipore, Darmstadt, Germany), aliquoted and directly used for crystallization or stored at −80◦C.
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bioRxiv - Cell Biology 2022Quote: ... and immunoreactive proteins were visualised using Immobilon® Forte Western HRP Substrate (Merck Millipore). Densitometric analysis was carried out using the ImageJ 1.53c software ...
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bioRxiv - Biophysics 2022Quote: ... Protein expressions were induced by 1 mM isopropyl β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) overnight at 25°C ...
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bioRxiv - Biophysics 2022Quote: ... and NpSxph I559Y) Protein samples were filtered through a 0.22 µm spin filter (Millipore) before loading into the sample cell and titrated with STX (300 µM STX for RcSxph D794 and 100 µM STX for RcSxph ...
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bioRxiv - Cancer Biology 2022Quote: ... PBS and 1 μg/mL Human Plasma Fibronectin Purified Protein (Sigma-Aldrich, Cat. #: FC010). 1N NaOH was added to achieve a neutral ∼pH and a thin base layer of 100 μL volume was added to the well of a 48-well tissue culture plate precoated with 1% agarose ...
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bioRxiv - Cell Biology 2022Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K MWCO concentrator (EMD Millipore) to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated proteins were transferred on to 0.45 µm PVDF membrane (Merk Millipore, #IPVH00010), followed by blocking with 5 % skim milk in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration for lysates was determined using BCA reagent (Sigma-Aldrich, St. Louis, MO), and equal amounts of protein (50 μg/lane ...
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bioRxiv - Cell Biology 2022Quote: RIP assay was done with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). Quantitative RT-PCR was used for determining total RNA levels.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was eluted with sonication buffer containing 50 mM reduced glutathione (Sigma-Aldrich) and dialyzed against storage buffer [50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were developed using Luminata Classico or Luminata Forte HRP substrate (Millipore Sigma) and detected using an Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Tiam1 protein was purified using the ANTI-FLAG® M2 affinity gel (Sigma-Aldrich) followed by Superdex 75 ...
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bioRxiv - Cell Biology 2022Quote: Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein extracts were incubated with 5 μl T7 antibody (EMD Millipore; Cat # 69522) for 4 h ...